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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/08/1995 |
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Data da última atualização: |
31/08/1995 |
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Autoria: |
BOHER, B.; KPEMOUA, K.; NICOLE, M.; LUISETTI, J.; GEIGER, J. P. |
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Afiliação: |
ORSTOM, Laboratoire de Phytopathologie, B.P. 5045, 34032 Montpellier, France; fourty author:INRA, Station de Pathologie Vegetale et Phytobacteriologie, B.P. 57, 49071 Beaucouze Cedex, France. |
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Título: |
Ultrastructure of Interactions between cassava and xanthomonas campestris pv. manihotis: Cytochemistry of cellulose and pectin degradation in a susceptible cultivar. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Phytopathology, v.85, n.7, p.777-788, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
A cytochemical investigation was carried out on the development of an aggressive strain of Xanthomonas campestris pv. manihotis, responsible for the cassava bacterial blight, to gain better insights into molecular and cellular mechanisms involved in leaf cell wall degradation by this pathogen. The use of anti-pectin monoclonal antibodies revealed that the plant middle lamellae were damaged during the infection process, from the epiphytic stage on the leaf surface to invasion of vascular bundles. In parallel, application of a Beta-1,4-exoglucanase-gold probe to healthy and infected tissues indicated that primary and secondary cell walls were also altered. Quantitation of gold labeling confirmed that pectin was more severely degraded than cellulose. Accumulation of pectinlike compounds was also detected in occluded infected vessels. Bacterial-surrounding sheaths, which were coutinely seen during pathogenesis early after leaf inoculation, had a dense of loosened fibrillar appearance and were differentiated from the pathogen cell wall. Close association occurred between extracellular fibrils and leaf cell both at early and advanced stages of wall degradation. Bacterial extracellular sheaths were often seen deep in host cell walls, sometimes enclosing residual plant cell wall fragments. Our cytochemical data demonstrated that cell wall degradation of cassava by Xanthomonas campestris pv. manihotis plays an important role in host tissue colonization. |
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Palavras-Chave: |
Manihot esculenta crantz; Plant material; Tissue processing. |
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Thesaurus Nal: |
cytochemistry. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02148naa a2200217 a 4500
001 1634002
005 1995-08-31
008 1995 bl uuuu u00u1 u #d
100 1 $aBOHER, B.
245 $aUltrastructure of Interactions between cassava and xanthomonas campestris pv. manihotis$bCytochemistry of cellulose and pectin degradation in a susceptible cultivar.
260 $c1995
520 $aA cytochemical investigation was carried out on the development of an aggressive strain of Xanthomonas campestris pv. manihotis, responsible for the cassava bacterial blight, to gain better insights into molecular and cellular mechanisms involved in leaf cell wall degradation by this pathogen. The use of anti-pectin monoclonal antibodies revealed that the plant middle lamellae were damaged during the infection process, from the epiphytic stage on the leaf surface to invasion of vascular bundles. In parallel, application of a Beta-1,4-exoglucanase-gold probe to healthy and infected tissues indicated that primary and secondary cell walls were also altered. Quantitation of gold labeling confirmed that pectin was more severely degraded than cellulose. Accumulation of pectinlike compounds was also detected in occluded infected vessels. Bacterial-surrounding sheaths, which were coutinely seen during pathogenesis early after leaf inoculation, had a dense of loosened fibrillar appearance and were differentiated from the pathogen cell wall. Close association occurred between extracellular fibrils and leaf cell both at early and advanced stages of wall degradation. Bacterial extracellular sheaths were often seen deep in host cell walls, sometimes enclosing residual plant cell wall fragments. Our cytochemical data demonstrated that cell wall degradation of cassava by Xanthomonas campestris pv. manihotis plays an important role in host tissue colonization.
650 $acytochemistry
653 $aManihot esculenta crantz
653 $aPlant material
653 $aTissue processing
700 1 $aKPEMOUA, K.
700 1 $aNICOLE, M.
700 1 $aLUISETTI, J.
700 1 $aGEIGER, J. P.
773 $tPhytopathology$gv.85, n.7, p.777-788, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
17/05/2011 |
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Data da última atualização: |
28/06/2018 |
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Tipo da produção científica: |
Folder/Folheto/Cartilha |
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Autoria: |
WANDELLI, E. V.; SOUSA, G. F. de; MACEDO, J. L. V. de; SILVEIRA, I. B. da; ROCHA, A. S. N. da C. |
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Afiliação: |
ELISA VIEIRA WANDELLI, CPAA; JEFERSON LUIS VASCONCELOS DE MACEDO, CPAA; IRACINO BONFIM DA SILVEIRA, CPAA; ANTONIO SABINO NETO DA COSTA ROCHA, CPAA. |
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Título: |
Sistemas agroflorestais: alternativa sustentável para a agricultura familiar. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Manaus: Embrapa Amazônia Ocidental, 2001. |
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Descrição Física: |
1 folder. |
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Idioma: |
Português |
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Conteúdo: |
O que é sistema agroflorestal. Resolvendo o problema do solo pobre e do abandono da área pelo produtor. Como explicar que sobre solos pobres existe a Floresta Amazônica tão exuberante. Princípios e práticas dos sistemas agroflorestais. Vantagens dos sistemas agroflorestais. |
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Palavras-Chave: |
Agrofloresta. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179159/1/Folder-sistemas-agroflorest.pdf
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Marc: |
LEADER 00800nam a2200181 a 4500
001 1888751
005 2018-06-28
008 2001 bl uuuu u0uu1 u #d
100 1 $aWANDELLI, E. V.
245 $aSistemas agroflorestais$balternativa sustentável para a agricultura familiar.
260 $aManaus: Embrapa Amazônia Ocidental$c2001
300 $c1 folder.
520 $aO que é sistema agroflorestal. Resolvendo o problema do solo pobre e do abandono da área pelo produtor. Como explicar que sobre solos pobres existe a Floresta Amazônica tão exuberante. Princípios e práticas dos sistemas agroflorestais. Vantagens dos sistemas agroflorestais.
653 $aAgrofloresta
700 1 $aSOUSA, G. F. de
700 1 $aMACEDO, J. L. V. de
700 1 $aSILVEIRA, I. B. da
700 1 $aROCHA, A. S. N. da C.
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Embrapa Amazônia Ocidental (CPAA) |
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