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| Registros recuperados : 63 | |
| 1. |  | HUNGRIA, M.; MEGÍAS, M. Uma década de ouro se aproxima para a microbiologia do solo: expectativas da pesquisa, da indústria, dos agricultores e da sociedade. In: IBEROAMERICAN CONFERENCE ON BENEFICIAL PLANT - MICROORGANISM - ENVIRONMENT INTERACTIONS, 2.; NATIONAL MEETING OF THE SPANISH SOCIETY OF NITROGEN FIXATION, 14.; LATIN AMERICAN MEETING ON RHIZOBIOLOGY, 26.; SPANISH-PROTUGUESE CONGRESS ON NITROGEN FIXATION, 3., 2013, Sevilla. Microorganisms for future agriculture. Sevilla: Universidad de Sevilla; ALAR; SEFIN, 2013. p. 510-517.| Biblioteca(s): Embrapa Soja. |
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| 4. | | MEGÍAS, M.; MEGIAS, M. E.; REIS, F. B.; HUNGRIA, M.; OLLERO, F. J. Uso de metabolitos microbianos como aditivos en inoculantes. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Anais... [Brasília, DF]: Embrapa, 2018. resumo. p. 15. Título do evento por extenso: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIAS DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA, 19., 2018, Foz do Iguaçu.
Editores Técnicos: Jerri Édson Zilli, Fábio...| Biblioteca(s): Embrapa Soja. |
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| 6. | | MEGÍA, E.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. Draft genome sequence of Pantoea ananatis strain AMG521, a rice plant growth-promoting bacterial endophyte isolated from the Guadalquivir Marshes in Southern Spain. Genome Announcements, Washington, DC, v. 4, n. 1, p. 1-2, Jan./Feb. 2016.| Biblioteca(s): Embrapa Soja. |
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| 11. | | MEGÍAS, M. E.; PIRES, R. C.; MOYANO, I.; REIS JUNIOR, F. B. dos; OLLERO, F. J.; SIMON, M.; ZILLI, J. E.; MEGÍAS, M. Identification and characterization of rhizobia isolated from nodules of Mimosa spp.which efficiently nodulate Phaseolus vulgaris. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 20., 2017, Granada, Spain. Proceedings... Granada:University of Granada, 2017. p. 91| Biblioteca(s): Embrapa Agrobiologia. |
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| 12. | | MEGÍAS, M. E.; SOARES NETO, C. B.; ZILLI, J. E.; BAURA, A.; ANDRADE, L. M. de; VALE, H. M. M. do; MEGÍAS, M.; REIS JUNIOR, F. B. dos. Phylogenetic characterization of diazotrophic symbiotic bacteria isolated from Mimosa spp. plants growing in ultramafic soils. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 20., 2017, Granada, Spain. Proceedings... Granada:University of Granada, 2017. p. 90| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | FUKAMI, J.; NOGUEIRA, M. A.; MEGÍAS, M.; OLLERO MÁRQUEZ, F. J.; HUNGRIA, M. Atividade de enzimas antioxidantes em milho (Zea mays) submetidas à inoculação com azospirillum brasilense. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 190. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.| Biblioteca(s): Embrapa Soja. |
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| 15. | | ABRANTES, J. L. F.; FUKAMI, J.; MÉGIAS, M.; OLLERO MÁRQUEZ, F. J.; HUNGRIA, M. Blockade of N-acyl homoserine lactones-based quorum sensing impairs soybean-bradyrhizobium and maize-azospirillum symbiosis. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 176. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.| Biblioteca(s): Embrapa Soja. |
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| 16. | | FERNÁNDEZ, M.; MEGÍAS, E.; REIS JUNIOR, F. B. dos; MEGÍAS, M. Bacterial biodiversity within the genus Enterobacter in paddies of Guadalquivir marshes. In.: IBEROAMERICAN CONFERENCE ON BENEFICIAL PLANT - MICROORGANISM - ENVIRONMENT INTERACTIONS, 2.; NATIONAL MEETING OF THE SPANISH SOCIETY OF NITROGEN FIXATION, 14.; LATIN AMERICAN MEETING ON RHIZOBIOLOGY, 26.; SPANISH-PORTUGUESE CONGRESS ON NITROGEN FIXATION, 3., 2013, Sevilla. Microorganisms for future agriculture. Sevilla: Universidad de Sevilla; ALAR; SEFIN, 2013. p. 65-66.| Biblioteca(s): Embrapa Cerrados. |
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| 18. | | MEGIAS, E.; REIS JUNIOR, F. B.; RIBEIRO, R. A.; OLLERO, F. J.; MEGIAS, M.; HUNGRIA, M. Genome sequence of Pantoea ananatis strain amg 501, a plant growth-promoting bacterium isolated from rice leaves grown in paddies of southern spain. Genome Announcements, v. 5, n. 34, e00848-17, 2017. 2 p.| Biblioteca(s): Embrapa Cerrados; Embrapa Soja. |
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| 19. | | MEGIAS, E.; REIS JUNIOR, F. B.; RIBEIRO, R. A.; MEGIAS, M.; OLLERO, F. J.; HUNGRIA, M. Genome sequence of Pantoea sp. strain 1.19, isolated from rice rhizosphere, with the capacity to promote growth of legumes and nonlegumes. Genome Announcements, v. 5, n. 30, e00707-17, 2017.| Biblioteca(s): Embrapa Cerrados; Embrapa Soja. |
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| Registros recuperados : 63 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/03/2016 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; ESPUNY, M. del R.; RODRÍGUEZ-CARVAJAL, M. A.; SORIA-DÍAZ, M. E.; NAKATANI, A. S.; HUNGRIA, M.; JAVIER OLLERO, F.; MEGÍAS, M. |
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Afiliação: |
PABLO DEL CERRO, Universidad de Sevilla; AMANDA ALVES PAIVA ROLLA-SANTOS; DOUGLAS FABIANO GOMES; BETTINA BERQUÓ MARKS; MARÍA DEL ROSARIO ESPUNY, Universidad de Sevilla; MIGUEL ÁNGEL RODRÍGUEZ-CARVAJAL, Universidad de Sevilla; MARÍA EUGENIA SORIA-DÍAZ, CITIUS; ANDRÉ SHIGUEYOSHI NAKATAN; MARIANGELA HUNGRIA DA CUNHA, CNPSO; FRANCISCO JAVIER OLLERO; MANUEL MEGÍAS. |
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Título: |
Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC Genomics, v. 16, n. 1, p. 864, Oct. 2015. |
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ISSN: |
1471-2164 |
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DOI: |
10.1186/s12864-015-2033-z |
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Idioma: |
Inglês |
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Conteúdo: |
Transcription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes?five?and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. The three nodD mutants were built by insertion of ? interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-geneinducing molecule apigenin and of saline stress. No differences between the mutants and the WT were observed in exopolysaccharide (EPS) and lipopolysaccharide (LPS) profiles, motility, indole acetic acid (IAA) synthesis or biofilm production, either in the presence, or in the absence of inducers. Nodulation studies demonstrated the most complex regulatory system described so far, requiring from one (Leucaena leucocephala, Lotus burtii) to four (Lotus japonicus) nodD genes. Up to 38 different structures of Nod factors were detected, being higher under salt stress, except for the nodD5 mutant; in addition, a high number of structures was synthesized by the nodD4 mutant in the absence of any inducer. Probable activator (nodD3 and nodD5) or repressor roles (nodD4), possibly via nodD1 and/or nodD2, were attributed to the three nodD genes. Expression of nodC, nodD1 and each nodD studied by RT-qPCR confirmed that nodD3 is an activator of nodD1, both in the presence of apigenin and salt stress. In contrast, nodD4 might be an inducer with apigenin and a repressor under saline stress, whereas nodD5 was an inducer under both conditions. MenosTranscription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes?five?and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. The three nodD mutants were built by insertion of ? interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-geneinducing molecule apigenin and of saline stress. No differences... Mostrar Tudo |
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Thesagro: |
Bacteriologia do solo; Fixação de nitrogênio; Nodulação; Rhizobium. |
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Thesaurus NAL: |
Bacteriology; Nitrogen fixation; Nodulation. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140562/1/12864-2015-Article-2033.pdf
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Marc: |
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024 7 $a10.1186/s12864-015-2033-z$2DOI
100 1 $aCERRO, P. del
245 $aOpening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899.$h[electronic resource]
260 $c2015
520 $aTranscription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes?five?and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. The three nodD mutants were built by insertion of ? interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-geneinducing molecule apigenin and of saline stress. No differences between the mutants and the WT were observed in exopolysaccharide (EPS) and lipopolysaccharide (LPS) profiles, motility, indole acetic acid (IAA) synthesis or biofilm production, either in the presence, or in the absence of inducers. Nodulation studies demonstrated the most complex regulatory system described so far, requiring from one (Leucaena leucocephala, Lotus burtii) to four (Lotus japonicus) nodD genes. Up to 38 different structures of Nod factors were detected, being higher under salt stress, except for the nodD5 mutant; in addition, a high number of structures was synthesized by the nodD4 mutant in the absence of any inducer. Probable activator (nodD3 and nodD5) or repressor roles (nodD4), possibly via nodD1 and/or nodD2, were attributed to the three nodD genes. Expression of nodC, nodD1 and each nodD studied by RT-qPCR confirmed that nodD3 is an activator of nodD1, both in the presence of apigenin and salt stress. In contrast, nodD4 might be an inducer with apigenin and a repressor under saline stress, whereas nodD5 was an inducer under both conditions.
650 $aBacteriology
650 $aNitrogen fixation
650 $aNodulation
650 $aBacteriologia do solo
650 $aFixação de nitrogênio
650 $aNodulação
650 $aRhizobium
700 1 $aROLLA-SANTOS, A. A. P.
700 1 $aGOMES, D. F.
700 1 $aMARKS, B. B.
700 1 $aESPUNY, M. del R.
700 1 $aRODRÍGUEZ-CARVAJAL, M. A.
700 1 $aSORIA-DÍAZ, M. E.
700 1 $aNAKATANI, A. S.
700 1 $aHUNGRIA, M.
700 1 $aJAVIER OLLERO, F.
700 1 $aMEGÍAS, M.
773 $tBMC Genomics$gv. 16, n. 1, p. 864, Oct. 2015.
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