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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
18/08/1998 |
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Data da última atualização: |
12/02/2019 |
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Autoria: |
BAZONI, R.; FERNANDES, A. T. F.; COSTA, C. A.; GUIMARAES, E. P.; CARVALHO, J. R. P. de; SANTOS, A. B. dos. |
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Afiliação: |
REINALDO BANOZI, UNIDERP; ANDRÉIA TOSTES FILGUEIRAS FERNANDES, UNIDERP; CESAR AUGUSTO COSTA, UNIDERP; ELCIO PERPETUO GUIMARAES, CNPAF; JOSE RUY PORTO DE CARVALHO, CNPTIA; ALBERTO BAETA DOS SANTOS, CNPAF. |
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Título: |
Estudo do arranjo espacial de plantas da cultura do arroz de terras altas em Mato Grosso do Sul. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 6., 1998, Goiânia. Perspectivas para a cultura dos arroz nos ecossistemas de varzeas e terras altas: resumos expandidos. Goiânia: EMBRAPA-CNPAF, 1998. |
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Volume: |
v. 1 |
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Páginas: |
p. 33-36. |
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Série: |
(EMBRAPA-CNPAF, Documentos, 85). |
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Idioma: |
Português |
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Conteúdo: |
Na década de 70, com advento do programa de financiamentos agropecuários "Polocentro", a cultura de arroz de terras altas tomou grande impulso, especialmente no cerrado do Brasil Central, quando essa região tornou-se responsável pela maior área de cultivo nesse sistema. |
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Palavras-Chave: |
Delineamento experimental; Densidade e espacamento de arroz; Estatistica experimental; Experimental statistics; Rice density and spacing. |
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Thesaurus Nal: |
experimental design. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01293naa a2200277 a 4500
001 1006592
005 2019-02-12
008 1998 bl uuuu u00u1 u #d
100 1 $aBAZONI, R.
245 $aEstudo do arranjo espacial de plantas da cultura do arroz de terras altas em Mato Grosso do Sul.
260 $c1998
300 $ap. 33-36. v. 1
490 $a(EMBRAPA-CNPAF, Documentos, 85).$vv. 1
520 $aNa década de 70, com advento do programa de financiamentos agropecuários "Polocentro", a cultura de arroz de terras altas tomou grande impulso, especialmente no cerrado do Brasil Central, quando essa região tornou-se responsável pela maior área de cultivo nesse sistema.
650 $aexperimental design
653 $aDelineamento experimental
653 $aDensidade e espacamento de arroz
653 $aEstatistica experimental
653 $aExperimental statistics
653 $aRice density and spacing
700 1 $aFERNANDES, A. T. F.
700 1 $aCOSTA, C. A.
700 1 $aGUIMARAES, E. P.
700 1 $aCARVALHO, J. R. P. de
700 1 $aSANTOS, A. B. dos
773 $tIn: REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 6., 1998, Goiânia. Perspectivas para a cultura dos arroz nos ecossistemas de varzeas e terras altas: resumos expandidos. Goiânia: EMBRAPA-CNPAF, 1998.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/07/2004 |
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Data da última atualização: |
26/07/2017 |
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Autoria: |
PINEDO, F. J. R.; MOSCARDI, F.; LUQUE, T.; OLSZEWSKI, J. A.; RIBEIRO, B. M. |
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Título: |
Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Biological Control, v. 27, n. 3, p. 336-344, July 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Some baculovirus have been genetically modified for the inactivation of their ecdysteroid
glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type
AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A.
gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. |
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Palavras-Chave: |
Ecdysteroid glucosyltransferase; Velvetbean caterpillar. |
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Thesagro: |
Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Engenharia Genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02224naa a2200241 a 4500
001 1467192
005 2017-07-26
008 2003 bl --- 0-- u #d
100 1 $aPINEDO, F. J. R.
245 $aInactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host.
260 $c2003
520 $aSome baculovirus have been genetically modified for the inactivation of their ecdysteroid glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A. gemmatalis larvae (in bioassays) compared to wild-type AgMNPV.
650 $aAnticarsia Gemmatalis
650 $aBaculovirus
650 $aControle Biológico
650 $aEngenharia Genética
653 $aEcdysteroid glucosyltransferase
653 $aVelvetbean caterpillar
700 1 $aMOSCARDI, F.
700 1 $aLUQUE, T.
700 1 $aOLSZEWSKI, J. A.
700 1 $aRIBEIRO, B. M.
773 $tBiological Control$gv. 27, n. 3, p. 336-344, July 2003.
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