Data/hora: 25/06/2024 18:48:06

Biblioteca(s): | Embrapa Soja.
Data corrente: | 11/12/2013
Data da última atualização: | 03/08/2017
Autoria: | D'ALESSANDRO, C. P.; JONES, L. R.; HUMBER, R. A.; LÓPEZ LASTRA, C. C.; SOSA-GOMEZ, D. R.
Afiliação: | CELESTE P. D'ALESSANDRO, CEPAVE/CONICET-UNLP; LEANDRO R. JONES, CONICET; RICHARD A. HUMBER, USDA-ARS; CLAUDIA C. LÓPEZ LASTRA, CEPAVE/CONICET-UNLP; DANIEL RICARDO SOSA-GOMEZ, CNPSO.
Título: | Characterization and phylogeny of Isaria spp. strains (Ascomycota: Hypocreales) using ITS1-5.8S-ITS2 and elongation factor 1-alpha sequences.
Ano de publicação: | 2013
Fonte/Imprenta: | Journal of Basic Microbiology, Weinheim, v. 53, n. 12, p. 1-11, Nov. 2013.
DOI: | 10.1002/jobm.201300499
Idioma: | Inglês
Conteúdo: | The elongation factor 1-alpha (EF1-&#945;) and the internal transcribed spacer (ITS) regions ITS1 and ITS2 (ITS1-5.8S-ITS2) sequences were used to characterize and to identify Isaria isolates from Argentina, Mexico, and Brazil, as well as to study the phylogenetic relationships among these isolates and other related fungi from the order Hypocreales. The molecular characterization, which was performed by PCR-RFLP of EF1-&#945; and ITS1-5.8-ITS2 genes, was useful for resolving representative isolates of Isaria fumosorosea, Isaria farinosa, and Isaria tenuipes and to confirm the taxonomic identity of fungi from Argentina, Mexico, and Brazil. The phylogenetic analyses showed three clades corresponding to three families of Hypocreales. The genus Isaria was confirmed as polyphyletic and in family Cordycipitaceae, Isaria species were related to anamorphic species of Beauveria, Lecanicillium, and Simplicillium and to teleomorphic Cordyceps and Torrubiella. Therefore, EF1-&#945; and ITS1-5.8S-ITS2 genes were found to be powerful tools for improving the characterization, identification, and phylogenetic relationship of the Isaria species and other entomopathogenic fungi.
Thesagro: | Filogenia.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (34925-1) - UPC - AP - DD -  -  -  - DIS