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| Registros recuperados : 32 | |
| 4. |  | YAMADA, M. M.; FALEIRO, F. G.; LOPES, U. V.; FLORES, A. B.; PIRES, J. L. Genetic divergence in cocoa progenies for backcrossing program to witches’ broom disease resistance. Revista Ceres, Viçosa, v. 57, n. 1, p. 73-78, ja./fev., 2010.| Biblioteca(s): Embrapa Cerrados. |
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| 9. | | GRAMACHO, K. P.; RISTERUCCI, A. M.; LANAUD, C.; BAHIA, C.; LOPES, U. V.; GESTEIRA, A. da S.; ARAÚJO, M. R. Development of microsatelllte markers for the genetic analysis of Crinipellis perniciosa. Agrotrópica, v. 17, p61 - 64,dez. 2005.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | SANTOS, R. M. F.; LOPES, U. V.; BAHIA, R. de C.; MACHADO, R. C. R.; AHNERT, D.; CORRÊA, R. X. Marcadores microssatélites relacionados com a resistência à vassoura-de-bruxa do cacaueiro Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 8, p. 1137-1142, ago. 2007 Título em inglês: Microsatellite markers related to resistance of cocoa tree against witches?-broom.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | YAMADA, M. M.; FALEIRO, F. G.; CLEMENT, D. P. L.; LOPES, U. V.; PIRES, J. L.; MELO, G. R. P. Relationship between molecular markers and incompatibility in Theobroma cacao L. Agrotropica, v. 22, n. 2, p. 71-74, 2010.| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | FALEIRO, F. G.; PEREIRA, M. G.; CLEMENT, D.; LOPES, U. V.; YAMADA, M. M.; ARAÚJO, I. S.; PIRES, J. L.; PEREIRA, J. L. Mapping genes of resistance in cocoa. In: COMMON FUND FOR COMMODITIES. Project: The use of molecular biology techniques in search for varieties resistant to wotches' broom disease of cocoa. Amsterdam: CFC, 2010. Cap. 2. p. 34-52. (CFC Technical Paper, 55).| Biblioteca(s): Embrapa Cerrados. |
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| 14. | | YAMADA, M. M.; FALEIRO, F. G.; FLORES, A. B.; LOPES, U. V.; PIRES, J. L.; CORRÊA, R. X.; SANTOS, R. F. dos. Microsatellite diversity and heterozygosity of parents of a cocoa breeding population. Crop Breeding and Applied Biotechnology, Londrina, v. 9, n. 1, p. 17-22, Mar. 2009.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 15. | | YAMADA, M. M.; FALEIRO, F. G.; FLORES, A. B.; LOPES, U. V.; PIRES, J. L.; CORRÊA, R. X.; SANTOS, R. F. dos. Microsatellite diversity and heterozygosity of parents of a cocoa breeding population. Crop Breeding and Applied Biotechnology, v. 9, p. 17-22, 2009.| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | YAMADA, M. M.; FALEIRO, F. G.; LOPES, U. V.; BAHIA, R. C.; PIRES, J. L.; GOMES, L. M. C.; MELO, G. R. P. Genetic variability in cultivated cacao populations in Bahia, Brazil, detected by isozymes and RAPD markers. Crop Breeding and Applied Biotechnology, Londrina, v. 1, n. 4, p. 377-383, Dec. 2001.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 17. | | YAMADA. M. M.; FALEIRO, F. G.; LOPES, U. V.; PIRES, J. L.; DANTAS NETO, A.; FLORES, A. B.; FALEIRO, A. S. G. Genetic variability and heterozygosity of cocoa accessions of Parinari (Pa) population, based on microsatellite markers. Crop Breeding and Applied Biotechnology, Londrina, v. 3, n. 4, p. 289-295, Dec. 2003.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 18. | | ARAUJO, I. S.; SOUZA FILHO, G. A.; PEREIRA, M. G.; LOPES, U. V.; FALEIRO, F. G.; MACHADO, R. C.; QUEIROZ, V. T.; GUIMARAES, C. T.; BROWN, J. S. Identificação de QTLs em cacau (Theobroma cacao L.): qualidade da manteiga. In: CONGRESSO BRASILEIRO DE GENÉTICA, 49., 2003, Águas de Lindóia. A dupla hélice do DNA: [resumos]. Ribeirão Preto: Sociedade Brasileira de Genética, 2003. p. 678.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 19. | | YAMADA, M. M.; FLORES, A. B.; FALEIRO, F. G.; MELO, G. R. P.; MACEDO, M. M.; LOPES, U. V.; XAVIER, R. C.; SANTOS, R. F. dos. Identificação e variabilidade genética de acessos de cacaueiros autocompatíveis selecionados para resistência à vassoura-de-bruxa em fazendas produtoras. Agrotrópica, Itabuna, v. 17, p. 17-22, 2005. Publicado em dezembro de 2006.| Biblioteca(s): Embrapa Cerrados. |
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| 20. | | MARQUES, J. R. B.; MONTEIRO, W. R.; LOPES, U. V.; SERÔDIO, M. H. de C. F.; BRANDÃO, I. C. S. F. L. Avaliação do sistema agroflorestal zonal entre seringueira e cacaueiro na Região Sudeste do Estado da Bahia. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 5., 2004, Curitiba. SAFs: desenvolvimento com proteção ambiental: anais. Colombo: Embrapa Florestas, 2004. p. 365-367. (Embrapa Florestas. Documentos, 98).| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 32 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
30/03/2016 |
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Data da última atualização: |
17/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, L. F. dos; FREGAPANI, R. M.; FALCAO, L. L.; TOGAWA, R. C.; COSTA, M. M. do C.; LOPES, U. V.; GRAMACHO, K. P.; ALVES, R. M.; MICHELI, F.; MARCELLINO, L. H. |
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Afiliação: |
LUCAS FERRAZ DOS SANTOS; ROBERTA MOREIRA FREGAPANI; LOENI LUDKE FALCAO, CENARGEN; ROBERTO COITI TOGAWA, CENARGEN; MARCOS MOTA DO CARMO COSTA, CENARGEN; UILSON VANDERLEI LOPES, CEPLAC/CEPEC; KARINA PERES GRAMACHO, CEPLAC/CEPEC; RAFAEL MOYSES ALVES, CPATU; FABIENNE MICHELI, UESC/CIRAD; LUCILIA HELENA MARCELLINO, CENARGEN. |
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Título: |
First microsatellite markers developed from cupuassu ESTs: application in diversity analysis and cross-species transferability to cacao. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
PLoS ONE, v. 11, n. 3, e0151074, 2016. |
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DOI: |
10.1371/journal.pone.0151074 |
|
Idioma: |
Inglês |
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Conteúdo: |
The cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively. MenosThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cup... Mostrar Tudo |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Cacau; Cupuaçu; Fruta Tropical. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141829/1/2016Santos.pdf
|
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Marc: |
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024 7 $a10.1371/journal.pone.0151074$2DOI
100 1 $aSANTOS, L. F. dos
245 $aFirst microsatellite markers developed from cupuassu ESTs$bapplication in diversity analysis and cross-species transferability to cacao.$h[electronic resource]
260 $c2016
520 $aThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively.
650 $aCacau
650 $aCupuaçu
650 $aFruta Tropical
653 $aMelhoramento genético
700 1 $aFREGAPANI, R. M.
700 1 $aFALCAO, L. L.
700 1 $aTOGAWA, R. C.
700 1 $aCOSTA, M. M. do C.
700 1 $aLOPES, U. V.
700 1 $aGRAMACHO, K. P.
700 1 $aALVES, R. M.
700 1 $aMICHELI, F.
700 1 $aMARCELLINO, L. H.
773 $tPLoS ONE$gv. 11, n. 3, e0151074, 2016.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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