| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
|
Data corrente: |
02/10/2015 |
|
Data da última atualização: |
13/11/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
|
Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
|
Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
|
Páginas: |
16 p. |
|
DOI: |
10.1371/journal.pone.0139051 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
|
Palavras-Chave: |
Drought stress; Soybean. |
|
Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
|
Thesaurus Nal: |
Drought tolerance; Soybeans; Transcriptome. |
|
Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1079599/1/nepomucenoplosone.pdf
|
|
Marc: |
LEADER 02592naa a2200361 a 4500
001 2079599
005 2017-11-13
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0139051$2DOI
100 1 $aMARCOLINO-GOMES, J.
245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource]
260 $c2015
300 $a16 p.
520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day.
650 $aDrought tolerance
650 $aSoybeans
650 $aTranscriptome
650 $aEstiagem
650 $aResistência à seca
650 $aSeca
650 $aSoja
653 $aDrought stress
653 $aSoybean
700 1 $aRODRIGUES, F. A.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aFARIAS, J. R. B.
700 1 $aHARMON, F. G.
700 1 $aMOLINARI, H. B. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aNEPOMUCENO, A. L.
773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
09/12/2019 |
|
Data da última atualização: |
14/04/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
LOPES, S. R.; CRUZ, I. |
|
Afiliação: |
SINVAL RESENDE LOPES, CNPMS; IVAN CRUZ, CNPMS. |
|
Título: |
Management of Euxesta spp. in sweet corn with McPhail traps. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Neotropical Entomology, v. 49, n. 1, p. 139-146, 2020. |
|
DOI: |
10.1007/s13744-019-00733-1 |
|
Idioma: |
Inglês |
|
Notas: |
Publicado online em 8 dez. 2019. |
|
Conteúdo: |
Pests attacking the ear of sweet corn, such as Helicoverpa and Euxesta species,causeeconomiclosses fortheproducer and theprocessingindustry. Feeding on the style-stigmata preventing fertilization and on the developing grain and the association with pathogens are the main causes of product depreciation. The traditional control such as spraying with chemicals is not effective, even with several applications directed to the corn ear. Bacillus thuringiensis (Bt) corn also does not reach the fly. McPhail traps that have been used to monitor the pest can be a control strategy. This work evaluated the efficiency of food attractants placed inside McPhail traps to remove adult insects, in order to reduce ear damage. Twelve McPhail-type traps were installed in a randomized complete block designcontainingBioAnastrepha®aloneorcombinedwithdifferentdoses of insecticide. Every 10 days, all the captured insects were counted and separated by species and sex. Only Euxesta eluta and Euxesta mazorca were found. The occurrence of insects was greater in the period between silk emergence and grain filling. The number of females was higher, probably due to the need to feed before oviposition.Then umber of E.mazorca females caught in the treatment containing only Bio Anastrepha® was higher compared with that of others. The mean ear damage was very low, and there was no interaction between the production parameters and the distance between the trap and the harvested plant. In short, the useofMcPhailtrapcontainingfoodattractantsmaybeaviablealternative to control corn silk flies. MenosPests attacking the ear of sweet corn, such as Helicoverpa and Euxesta species,causeeconomiclosses fortheproducer and theprocessingindustry. Feeding on the style-stigmata preventing fertilization and on the developing grain and the association with pathogens are the main causes of product depreciation. The traditional control such as spraying with chemicals is not effective, even with several applications directed to the corn ear. Bacillus thuringiensis (Bt) corn also does not reach the fly. McPhail traps that have been used to monitor the pest can be a control strategy. This work evaluated the efficiency of food attractants placed inside McPhail traps to remove adult insects, in order to reduce ear damage. Twelve McPhail-type traps were installed in a randomized complete block designcontainingBioAnastrepha®aloneorcombinedwithdifferentdoses of insecticide. Every 10 days, all the captured insects were counted and separated by species and sex. Only Euxesta eluta and Euxesta mazorca were found. The occurrence of insects was greater in the period between silk emergence and grain filling. The number of females was higher, probably due to the need to feed before oviposition.Then umber of E.mazorca females caught in the treatment containing only Bio Anastrepha® was higher compared with that of others. The mean ear damage was very low, and there was no interaction between the production parameters and the distance between the trap and the harvested plant. In short, the useofMcPhailt... Mostrar Tudo |
|
Palavras-Chave: |
Atrativo alimentar. |
|
Thesagro: |
Controle Biológico; Milho; Mosca. |
|
Categoria do assunto: |
O Insetos e Entomologia |
|
URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1116274/1/Managementeuxesta.pdf
|
|
Marc: |
LEADER 02177naa a2200205 a 4500
001 2116274
005 2020-04-14
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s13744-019-00733-1$2DOI
100 1 $aLOPES, S. R.
245 $aManagement of Euxesta spp. in sweet corn with McPhail traps.$h[electronic resource]
260 $c2020
500 $aPublicado online em 8 dez. 2019.
520 $aPests attacking the ear of sweet corn, such as Helicoverpa and Euxesta species,causeeconomiclosses fortheproducer and theprocessingindustry. Feeding on the style-stigmata preventing fertilization and on the developing grain and the association with pathogens are the main causes of product depreciation. The traditional control such as spraying with chemicals is not effective, even with several applications directed to the corn ear. Bacillus thuringiensis (Bt) corn also does not reach the fly. McPhail traps that have been used to monitor the pest can be a control strategy. This work evaluated the efficiency of food attractants placed inside McPhail traps to remove adult insects, in order to reduce ear damage. Twelve McPhail-type traps were installed in a randomized complete block designcontainingBioAnastrepha®aloneorcombinedwithdifferentdoses of insecticide. Every 10 days, all the captured insects were counted and separated by species and sex. Only Euxesta eluta and Euxesta mazorca were found. The occurrence of insects was greater in the period between silk emergence and grain filling. The number of females was higher, probably due to the need to feed before oviposition.Then umber of E.mazorca females caught in the treatment containing only Bio Anastrepha® was higher compared with that of others. The mean ear damage was very low, and there was no interaction between the production parameters and the distance between the trap and the harvested plant. In short, the useofMcPhailtrapcontainingfoodattractantsmaybeaviablealternative to control corn silk flies.
650 $aControle Biológico
650 $aMilho
650 $aMosca
653 $aAtrativo alimentar
700 1 $aCRUZ, I.
773 $tNeotropical Entomology$gv. 49, n. 1, p. 139-146, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
