Data/hora: 23/09/2024 14:11:02

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	06/06/2023	
Data da última atualização: 	22/08/2023	
Autoria: 	SIQUEIRA, J. T. da C.; REIS, A. C.; LOPES, J. M. L.; LADEIRA, L. O.; VICCINI, L. F.; BRANDAO, H. de M.; MUNK, M.; SOUSA, S. M. de.	
Afiliação: 	JULIANA TATIARA DA COSTA SIQUEIRA, Universidade Federal de Juiz de Fora; ARYANE CAMPOS REIS, Universidade Federal de Juiz de Fora; JULIANA MAINENTI LEAL LOPES, University of Trás-os-Montes and Alto Douro; LUIZ ORLANDO LADEIRA, Universidade Federal de Minas Gerais; LYDERSON FACIO VICCINI, Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, Universidade Federal de Juiz de Fora; SAULO MARÇAL DE SOUSA, Universidade Federal de Juiz de Fora.	
Título: 	Chromosomal aberrations and changes in the methylation patterns of Lactuca sativa L. (Asteraceae) exposed to carbon nanotubes.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Biologia, v. 78, p. 1991-2002, 2023.	
DOI: 	https://doi.org/10.1007/s11756-023-01325-6	
Idioma: 	Inglês	
Conteúdo: 	Carbon nanotubes (CNTs) are one of the most important nanomaterials, with applications in several areas. The exponential increase in the production and use of CNTs, however, increases the likelihood of their release into the environment and potential impacts on environmental systems. We evaluated the cytogenotoxic potential of carboxylated multi-walled carbon nanotubes (MWCNT-COOH) as well as its influence in the methylation patterns of Lactuca sativa meristematic cells. Root elongation was observed at concentrations of 1.0; 10.0; 50.0 and 100.0 &#956;g/mL, and decreased seed germination at the highest concentration evaluated (100 ug/mL). Cytogenetic analysis revealed that MWCNT-COOH induced increases in the chromosomal aberration index and micronucleus frequency. Accumulations of cells in the sub G1 phase were also observed at low concentrations (0.1 &#956;g / mL), which may be related to increased programmed cell death. Cytosine methylation profile analysis revealed cytosine hypermethylation at MWCNT-COOH concentrations of 1.0 and 10.0 &#956;g / mL and cytosine hypomethylation at MWCNT-COOH concentrations of 50.0 and 100.0 &#956;g / mL as compared to controls. We conclude that, in addition to cytogenotoxic effects, MWCNT-COOH alters cytosine methylation profiles and promotes root elongation.	
Palavras-Chave: 	Citogenotoxicidade; Citometria de fluxo; Morte celular; Nanotubo de carbono.	
Thesagro: 	Alface.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (26032-1) - UPC - AP - DD -  -  -  - DIS