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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
18/03/2016 |
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Data da última atualização: |
14/02/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GOMES, J. M.; NAKAYAMA, T. J.; REIS, R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; MOLINARI, H. B. C.; HARMON, F.; NEPOMUCENO, A. L. |
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Afiliação: |
JULIANA MARCOLINO GOMES, UEL; THIAGO J. NAKAYAMA, UFV; RAFAELA RIBEIRO REIS, UEL; FABIANA APARECIDA RODRIGUES; LILIANE MARCIA MERTZ HENNING, CNPSO; RENATA FUGANTI PAGLIARINI; HUGO BRUNO CORREA MOLINARI, CNPAE; FRANK HARMON, USDA; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Gene expression and functional analysis of soybean genes with diurnal oscillation during drought stress. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME XXIII, 2015, San Diego. |
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Páginas: |
PO 203 |
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Idioma: |
Inglês |
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Conteúdo: |
Drought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants survival in response to drought. Together our results suggest that we identified key genes in drought tolerance. Further studies of the transgenic plants will help to unveil the drought tolerance mechanisms, contributing to the development of drought tolerant soybeans. MenosDrought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants ... Mostrar Tudo |
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Thesagro: |
Gene; Resistência à seca; Soja. |
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Thesaurus Nal: |
Soybeans; Water stress. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141414/1/Gene-expression-and-functional-...-2015.html
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Marc: |
LEADER 02573nam a2200277 a 4500
001 2041429
005 2018-02-14
008 2015 bl uuuu u00u1 u #d
100 1 $aGOMES, J. M.
245 $aGene expression and functional analysis of soybean genes with diurnal oscillation during drought stress.$h[electronic resource]
260 $aIn: PLANT & ANIMAL GENOME XXIII, 2015, San Diego.$c2015
300 $aPO 203
520 $aDrought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants survival in response to drought. Together our results suggest that we identified key genes in drought tolerance. Further studies of the transgenic plants will help to unveil the drought tolerance mechanisms, contributing to the development of drought tolerant soybeans.
650 $aSoybeans
650 $aWater stress
650 $aGene
650 $aResistência à seca
650 $aSoja
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aRODRIGUES, F. A.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aMOLINARI, H. B. C.
700 1 $aHARMON, F.
700 1 $aNEPOMUCENO, A. L.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
18/09/1997 |
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Data da última atualização: |
02/09/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
NOGUEIRA, A. R. de A.; LOPES, G. S.; LIMA, E. C.; WANDERLEY, R. D. C.; SILVA, A. G. DA; KRUG, F. J. |
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Afiliação: |
ANA RITA DE ARAUJO NOGUEIRA, CPPSE; G. S. LOPES, UNIVERSITY OF ARIZONA; E. C. LIMA, UNIVERSITY OF ARIZONA; RUY DA CARVALHEIRA WANDERLEY, CPPSE; ALIOMAR GABRIEL DA SILVA, CPPSE; F. J .KRUG, USP CENA. |
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Título: |
Determinação do fluxo de múltiplos marcadores de digesta em carneiros utilizando técnicas alternativas de análise. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: SBZ, 1997. |
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Páginas: |
p.134-136 |
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Idioma: |
Português |
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Conteúdo: |
Um experimento foi desenvolvido para avaliar a utilização de técnicas analíticas alternativas na determinação da taxa de passagem ruminal (turnover) de diferentes marcadores de digesta em carneiros deslanados na raça Santa Inês. |
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Palavras-Chave: |
Absorção atômica; Análise por injeção em fluxo; Atomização eletrotérmica; Filamento de tungstênio; Itérbio; Taxa de passagem. |
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Thesagro: |
Cobalto; Cromo. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/118743/1/digitalizar0029.pdf
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Marc: |
LEADER 01155nam a2200277 a 4500
001 1043880
005 2021-09-02
008 1997 bl uuuu u00u1 u #d
100 1 $aNOGUEIRA, A. R. de A.
245 $aDeterminação do fluxo de múltiplos marcadores de digesta em carneiros utilizando técnicas alternativas de análise.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: SBZ$c1997
300 $ap.134-136
520 $aUm experimento foi desenvolvido para avaliar a utilização de técnicas analíticas alternativas na determinação da taxa de passagem ruminal (turnover) de diferentes marcadores de digesta em carneiros deslanados na raça Santa Inês.
650 $aCobalto
650 $aCromo
653 $aAbsorção atômica
653 $aAnálise por injeção em fluxo
653 $aAtomização eletrotérmica
653 $aFilamento de tungstênio
653 $aItérbio
653 $aTaxa de passagem
700 1 $aLOPES, G. S.
700 1 $aLIMA, E. C.
700 1 $aWANDERLEY, R. D. C.
700 1 $aSILVA, A. G. DA
700 1 $aKRUG, F. J.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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