Data/hora: 21/06/2024 07:47:33

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Meio Ambiente.
Data corrente: | 06/05/2020
Data da última atualização: | 06/05/2020
Autoria: | LOPES, A. R. de O.; LOCATELLI, G. O.; BARBOSA, R. de M.; LOBO JUNIOR, M.; MASCARIN, G. M.; FINKLER, C. L. L.
Afiliação: | ALLYSON RODRIGO DE OLIVEIRA LOPES, UNIVERSIDADE FEDERAL DE PERNAMBUCO; GABRIEL OLIVO LOCATELLI, UNIVERSIDADE FEDERAL DE PERNAMBUCO; RAQUEL DE MELO BARBOSA, UNINASSAU; MURILLO LOBO JUNIOR, CNPAF; GABRIEL MOURA MASCARIN, CNPMA; CHRISTINE LAMENHA LUNA FINKLER, UNIVERSIDADE FEDERAL DE PERNAMBUCO.
Título: | Preparation, characterisation and cell viability of encapsulated Trichoderma asperellumin alginate beads.
Ano de publicação: | 2020
Fonte/Imprenta: | Journal of Microencapsulation, v. 37, n. 3, p. 270-282, 2020.
ISSN: | 0265-2048
DOI: | https://doi.org/10.1080/02652048.2020.1729884
Idioma: | Inglês
Conteúdo: | Aim: The encapsulation of Trichoderma asperellum BRM-29104 using Ca-alginate matrix was evaluated. Methods: Intact and freeze-dried beads containing submerged conidia and microsclerotia (MS) of T. asperellum grown in liquid culture were prepared and characterised. Beads were stored at 8, 25, and 35 oC over 120 days. Results: The mean sizes of beads before and after freeze-drying were 2.5 ± 0.2mm and 1.5 x 1.1mm (± 0.1mm), respectively. Freeze-dried beads stored at 8 oC were more effective in maintaining conidia concentration, while MS concentrations yielded 102 MS/g for both beads at 8 and 25 oC. The concentration of viable cells in freeze-dried beads stored at 8 oC attained 3.0 x 108 CFU/g after 120 days. FIRT analysis showed an interaction between the alginate and the cell wall of the fungus. Conclusion: These findings support the use of alginate beads followed by freeze drying and cold storage for maintenance of viability of T. asperellum.
Palavras-Chave: | Biocontrol; Microsclerotia.
Thesagro: | Fungo; Trichoderma.
Thesaurus Nal: | Alginates; Encapsulation; Fungi; Trichoderma asperellum.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Arroz e Feijão (CNPAF)

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