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| Registros recuperados : 392 | |
| Registros recuperados : 392 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Meio Ambiente. |
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Data corrente: |
13/09/2022 |
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Data da última atualização: |
14/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LOCATELLI, G. O.; PIMENTEL, M. F.; BUENO, L. A.; LOBO JUNIOR, M.; MASCARIN, G. M.; FINKLER, C. L. L. |
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Afiliação: |
GABRIEL OLIVO LOCATELLI, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARIA FERNANDA PIMENTEL, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LUCIANO AVALLONE BUENO, UNIVERSIDADE FEDERAL DO ABC; MURILLO LOBO JUNIOR, CNPAF; GABRIEL MOURA MASCARIN, CNPMA; CHRISTINE LAMENHA LUNA FINKLER, UNIVERSIDADE FEDERAL DE PERNAMBUCO. |
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Título: |
Production of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biocatalysis and Agricultural Biotechnology, v. 44, article 102455, 2022. |
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ISSN: |
1878-8181 |
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DOI: |
https://doi.org/10.1016/j.bcab.2022.102455 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development. |
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Palavras-Chave: |
Autolyzed yeast; Biofungicides; C N ratio; Microsclerotia. |
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Thesagro: |
Carbono; Farinha; Fermentação; Fungo Para Controle Biológico; Nitrogênio; Sorgo; Trichoderma. |
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Thesaurus NAL: |
Biological control agents; Liquid state fermentation; Sorghum flour; Trichoderma asperellum. |
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Categoria do assunto: |
H Saúde e Patologia
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02407naa a2200385 a 4500
001 2146414
005 2022-09-14
008 2022 bl uuuu u00u1 u #d
022 $a1878-8181
024 7 $ahttps://doi.org/10.1016/j.bcab.2022.102455$2DOI
100 1 $aLOCATELLI, G. O.
245 $aProduction of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources.$h[electronic resource]
260 $c2022
520 $aAbstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development.
650 $aBiological control agents
650 $aLiquid state fermentation
650 $aSorghum flour
650 $aTrichoderma asperellum
650 $aCarbono
650 $aFarinha
650 $aFermentação
650 $aFungo Para Controle Biológico
650 $aNitrogênio
650 $aSorgo
650 $aTrichoderma
653 $aAutolyzed yeast
653 $aBiofungicides
653 $aC N ratio
653 $aMicrosclerotia
700 1 $aPIMENTEL, M. F.
700 1 $aBUENO, L. A.
700 1 $aLOBO JUNIOR, M.
700 1 $aMASCARIN, G. M.
700 1 $aFINKLER, C. L. L.
773 $tBiocatalysis and Agricultural Biotechnology$gv. 44, article 102455, 2022.
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Embrapa Arroz e Feijão (CNPAF) |
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