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Registros recuperados : 184 | |
Registros recuperados : 184 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
12/04/2018 |
Data da última atualização: |
08/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
MOREIRA, R. F.; MATOS, M. N. C.; ALVES FILHO, J. G.; VALLE, R. V. do; ELOY, A. M. X.; PINTO, T. M. F.; MACHADO, S. P.; COSTA, C. R. R.; LIMA FILHO, J. L. de; LIMA, J. P. M. S.; CUNHA, R. M. S. da. |
Afiliação: |
Raulzito Fernandes Moreira, Pós-graduação - Universidade Federal do Ceará (UFC) - Sobral, CE, Brasil.; Maria Nágila Carneiro Matos; João Garcia Alves Filho; Roberta Vianna do Valle; ANGELA MARIA XAVIER ELOY, CNPC; Tatiana Maria Farias Pinto; Saris Pinto Machado Junior; Cíntia Renata Rocha Costa; José Luiz de Lima Filho; João Paulo Matos Santos Lima; Rodrigo Maranguape Silva da Cunha. |
Título: |
Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 15, n. 1, p. 84-92, jan./mar. 2018. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations. |
Palavras-Chave: |
Análise proteômica; Isolation methods; Proteomic analysis; Proteomic profiles; Raça Moxotó. |
Thesagro: |
Caprino; Detergente. |
Thesaurus NAL: |
detergents; Goats; proteomics; Spermatozoa; Surfactants. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/175677/1/CNPC-Diversity-2018-.pdf
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Marc: |
LEADER 02033naa a2200385 a 4500 001 2090515 005 2019-04-08 008 2018 bl uuuu u00u1 u #d 100 1 $aMOREIRA, R. F. 245 $aDiversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods.$h[electronic resource] 260 $c2018 520 $aAbstract: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations. 650 $adetergents 650 $aGoats 650 $aproteomics 650 $aSpermatozoa 650 $aSurfactants 650 $aCaprino 650 $aDetergente 653 $aAnálise proteômica 653 $aIsolation methods 653 $aProteomic analysis 653 $aProteomic profiles 653 $aRaça Moxotó 700 1 $aMATOS, M. N. C. 700 1 $aALVES FILHO, J. G. 700 1 $aVALLE, R. V. do 700 1 $aELOY, A. M. X. 700 1 $aPINTO, T. M. F. 700 1 $aMACHADO, S. P. 700 1 $aCOSTA, C. R. R. 700 1 $aLIMA FILHO, J. L. de 700 1 $aLIMA, J. P. M. S. 700 1 $aCUNHA, R. M. S. da 773 $tAnimal Reproduction, Belo Horizonte$gv. 15, n. 1, p. 84-92, jan./mar. 2018.
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Embrapa Caprinos e Ovinos (CNPC) |
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