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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
27/11/2009 |
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Data da última atualização: |
19/08/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, L. C. da; CHAVARRIA, G.; MARODIN, G. A. B.; SANTOS, H. P. dos; ANTONIOLLI, L. R.; SOUZA, D. A.; ZART, M. |
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Afiliação: |
LEONARDO CURY DA SILVA, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; GERALDO CHAVARRIA, UNIVERSIDADE DE PASSO FUNDO; GILMAR ARDUÍNO BETTIO MARODIN, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; HENRIQUE PESSOA DOS SANTOS, CNPUV; LUCIMARA ROGERIA ANTONIOLLI, CNPUV; DANIEL ANTUNES SOUZA, CNPUV; MARCELO ZART, CNPUV (bolsista). |
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Título: |
Aplicação de cálcio e silício sobre maturação fisiológica e rresistência de bagas em uvas finas de mesa cv. BRS Morena sob cultivo protegido |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESO LATINOAMERICANO DE VITICULTURA Y ENOLOGIA, 12., 2009, [Montevideo]. [Anais...] [Montevideo]: Asociación de Enólogos del Uruguay, 2009. |
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Páginas: |
Não paginado. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
O cálcio e o silício são elementos diretamente relacionados à qualidade dos frutos por atuarem na estrutura e funcionamento da parede celular e membranas. Como consequencia, são responsáveis pela regulação da resistência ou suscetibilidade dos frutos à rachaduras de casca. O presente trabalho teve como objetivo a avaliação do controle de rachaduras no período de maturação pela aplicação de diferentes doses de cálcio e silício nos cachos de uvas finas de mesa. O ensaio foi conduzido no ciclo 2008/09 em um vinhedo de cinco anos da cultivar apirênica BRS Morena (Vitis vinifera L.), enxertadas sobre SO4, conduzidas em latada descontínua e sob cobertura plástica nas linhas de cultivo, na indicação de procedência Vale dos Vinhedos, Bento Gonçalves-RS, Brasil (29°20?21??S e 51°55?07??W) a 565,6 metros de altitude. |
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Thesagro: |
Cálcio; Cultivo protegido; Fisiologia vegetal; Maturação; Resistência; Silício; Uva; Viticultura. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/576439/1/ID11815.pdf
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Marc: |
LEADER 01821nam a2200289 a 4500
001 1576439
005 2022-08-19
008 2009 bl uuuu u00u1 u #d
100 1 $aSILVA, L. C. da
245 $aAplicação de cálcio e silício sobre maturação fisiológica e rresistência de bagas em uvas finas de mesa cv. BRS Morena sob cultivo protegido$h[electronic resource]
260 $aIn: CONGRESO LATINOAMERICANO DE VITICULTURA Y ENOLOGIA, 12., 2009, [Montevideo]. [Anais...] [Montevideo]: Asociación de Enólogos del Uruguay$c2009
300 $aNão paginado.$c1 CD-ROM.
520 $aO cálcio e o silício são elementos diretamente relacionados à qualidade dos frutos por atuarem na estrutura e funcionamento da parede celular e membranas. Como consequencia, são responsáveis pela regulação da resistência ou suscetibilidade dos frutos à rachaduras de casca. O presente trabalho teve como objetivo a avaliação do controle de rachaduras no período de maturação pela aplicação de diferentes doses de cálcio e silício nos cachos de uvas finas de mesa. O ensaio foi conduzido no ciclo 2008/09 em um vinhedo de cinco anos da cultivar apirênica BRS Morena (Vitis vinifera L.), enxertadas sobre SO4, conduzidas em latada descontínua e sob cobertura plástica nas linhas de cultivo, na indicação de procedência Vale dos Vinhedos, Bento Gonçalves-RS, Brasil (29°20?21??S e 51°55?07??W) a 565,6 metros de altitude.
650 $aCálcio
650 $aCultivo protegido
650 $aFisiologia vegetal
650 $aMaturação
650 $aResistência
650 $aSilício
650 $aUva
650 $aViticultura
700 1 $aCHAVARRIA, G.
700 1 $aMARODIN, G. A. B.
700 1 $aSANTOS, H. P. dos
700 1 $aANTONIOLLI, L. R.
700 1 $aSOUZA, D. A.
700 1 $aZART, M.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
30/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
NAIARA ZOCCAL SARAIVA, CPATU; CLARA SLADE OLIVEIRA, CNPGL; Cláudia Lima Verde Leal, USP; Marina Ragagnin de Lima, UNESP; Maite Del Collado, UNESP / USP; Roberta Vantini, UNESP; Fabio Morato Monteiro, Centro APTA Bovinos de Corte, Instituto de Zootecnia; SIMONE CRISTINA MEO NICIURA, CPPSE; Joaquim Mansano Garcia, UNESP. |
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Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, Dec. 2015. |
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DOI: |
http://dx.doi.org/10.1017/S0967199414000537 |
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Idioma: |
Inglês |
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Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
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Thesaurus NAL: |
chromatin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02641naa a2200289 a 4500
001 2027313
005 2022-05-30
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1017/S0967199414000537$2DOI
100 1 $aSARAIVA, N. Z.
245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource]
260 $c2015
520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos.
650 $achromatin
653 $aBovine
653 $aChemically induced enucleation
653 $aMicrotubule
653 $aNuclear transfer
700 1 $aOLIVEIRA, C. S.
700 1 $aLEAL, C. L. V.
700 1 $aLIMA, M. R. de
700 1 $aCALLADO, M. del
700 1 $aVANTINI, R.
700 1 $aMONTEIRO, F. M.
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 23, n. 6, p. 852-862, Dec. 2015.
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