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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
05/09/1995 |
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Data da última atualização: |
05/09/1995 |
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Autoria: |
CASTRO, V. L. S. S.; CAPALBO, D. M. F.; SOARES, C. M.; COSTA, F. P. |
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Afiliação: |
EMBRAPA-CNPMA. |
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Título: |
Identification of adverse effects of bacterial agents in mammals. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 7., 1995, Santos. Abstracts. Sao Paulo: Sociedade Brasileira de Microbiologia, 1995. |
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Páginas: |
n.p. |
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Idioma: |
Inglês |
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Conteúdo: |
A protocol of toxicological tests for registration of microbial pest-control agents (MPCA) has been recently proposed in Brazil. Its objective is to determine detrimental effects to humans and domestic animals, and should be performed in a sequence of three tiers. The first one provides an evaluation of the potential risks of the MPCA due to pathogenicity, infectivity and toxicity by an acute exposition. A standard methodology for such tests is needed, and it was the concern of this study. Rats were housed in metabolic cages at controlled conditions. The MPCA Bacillus thuringiensis var. kurstaki (Bt) was gavage or instilled in one dose containing 10.8 spores. It was conducted periodical clinical and microbial observations after dosing. When no Bt was noticed the feces anymore, gross necropsy of all animals was conducted. The rats have not presented neither clinical or pathological alterations nor body weight loss. The protocol hereby described showed to be useful for the mean purpose. It could be followed to bacterial agents as an easily make one instead of immunological assays or DNA probes to estimate clearence of the agent after the administration in the feces. |
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Palavras-Chave: |
Bioinsecticide; Crop protection; Protocol; Toxicological test. |
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Thesagro: |
Bacillus Thuringiensis. |
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Thesaurus Nal: |
environmental impact; risk analysis; risk assessment. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01986naa a2200265 a 4500
001 1012304
005 1995-09-05
008 1995 bl uuuu u00u1 u #d
100 1 $aCASTRO, V. L. S. S.
245 $aIdentification of adverse effects of bacterial agents in mammals.
260 $c1995
300 $an.p.
520 $aA protocol of toxicological tests for registration of microbial pest-control agents (MPCA) has been recently proposed in Brazil. Its objective is to determine detrimental effects to humans and domestic animals, and should be performed in a sequence of three tiers. The first one provides an evaluation of the potential risks of the MPCA due to pathogenicity, infectivity and toxicity by an acute exposition. A standard methodology for such tests is needed, and it was the concern of this study. Rats were housed in metabolic cages at controlled conditions. The MPCA Bacillus thuringiensis var. kurstaki (Bt) was gavage or instilled in one dose containing 10.8 spores. It was conducted periodical clinical and microbial observations after dosing. When no Bt was noticed the feces anymore, gross necropsy of all animals was conducted. The rats have not presented neither clinical or pathological alterations nor body weight loss. The protocol hereby described showed to be useful for the mean purpose. It could be followed to bacterial agents as an easily make one instead of immunological assays or DNA probes to estimate clearence of the agent after the administration in the feces.
650 $aenvironmental impact
650 $arisk analysis
650 $arisk assessment
650 $aBacillus Thuringiensis
653 $aBioinsecticide
653 $aCrop protection
653 $aProtocol
653 $aToxicological test
700 1 $aCAPALBO, D. M. F.
700 1 $aSOARES, C. M.
700 1 $aCOSTA, F. P.
773 $tIn: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 7., 1995, Santos. Abstracts. Sao Paulo: Sociedade Brasileira de Microbiologia, 1995.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido; Embrapa Uva e Vinho. |
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Data corrente: |
17/09/2013 |
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Data da última atualização: |
25/04/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NATIVIDADE, M. M. P.; CORREA, L. C.; SOUZA, S. V. C. de; PEREIRA, G. E.; LIMA, L. C. de O. |
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Afiliação: |
MARIANA MIRELLE PEREIRA NATIVIDADE, Universidade Federal de Lavras; LUIZ CLAUDIO CORREA, CPATSA; SCHEILLA VITORINO CARVALHO DE SOUZA, Universidade Federal de Minas Gerais; GIULIANO ELIAS PEREIRA, CNPUV / CPATSA; LUIZ CARLOS DE OLIVEIRA LIMA, Universidade Federal de Lavras. |
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Título: |
Simultaneous analysis of 25 phenolic compounds in grape juice for HPLC: Method validation and characterization of São Francisco Valley samples. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Microchemical Journal, New York, v. 110, p. 665-674, abr. 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
A HPLCmethod for simultaneous determination of 25 phenolic compounds in grape juicewas optimized, validated and applied in the characterization of juices produced in São Francisco Valley (SFV), Brazil. The performance characteristics of the method were established by assays with standard solutions of phenolic compounds, spiked and unspiked samples. Linearity,matrix effects, trueness, precision, detection and quantification limits were evaluated. Linearity was demonstrated in the concentration ranges tested for all phenolic compounds. Significant matrix effects were not identified for the studied compounds.Mean recoveries ranged from86.18 to 106.50%, demonstrating no lack of trueness. Precision of the method was confirmed for the 25 phenolic compounds, with acceptable repeatability relative standard deviations (from 0.71 to 9.24%) and within-reproducibility relative standard deviations (from1.34 to 9.26%) for unspiked and spiked samples. The theoretical limits of detection and quantification of themethod varied from 0.001 to 0.19 Mg mL-1 and 0.003 to 0.37 Mg mL-1, respectively. The results of the validation process showed that the proposed method is fitness for purpose. This method was able to identify simultaneously 25 phenolic compounds and had advantages such as low consumption of solvents and easy sample preparation. The phenolic profile of the grape juices from SFV varied according the grape cultivar. Phenolics of the anthocyanins and tannins class predominated in red grape juices, while in white grape juice phenolic acids and tannins were found at high concentrations. MenosA HPLCmethod for simultaneous determination of 25 phenolic compounds in grape juicewas optimized, validated and applied in the characterization of juices produced in São Francisco Valley (SFV), Brazil. The performance characteristics of the method were established by assays with standard solutions of phenolic compounds, spiked and unspiked samples. Linearity,matrix effects, trueness, precision, detection and quantification limits were evaluated. Linearity was demonstrated in the concentration ranges tested for all phenolic compounds. Significant matrix effects were not identified for the studied compounds.Mean recoveries ranged from86.18 to 106.50%, demonstrating no lack of trueness. Precision of the method was confirmed for the 25 phenolic compounds, with acceptable repeatability relative standard deviations (from 0.71 to 9.24%) and within-reproducibility relative standard deviations (from1.34 to 9.26%) for unspiked and spiked samples. The theoretical limits of detection and quantification of themethod varied from 0.001 to 0.19 Mg mL-1 and 0.003 to 0.37 Mg mL-1, respectively. The results of the validation process showed that the proposed method is fitness for purpose. This method was able to identify simultaneously 25 phenolic compounds and had advantages such as low consumption of solvents and easy sample preparation. The phenolic profile of the grape juices from SFV varied according the grape cultivar. Phenolics of the anthocyanins and tannins class predominated in red gr... Mostrar Tudo |
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Palavras-Chave: |
Composto fenológico; HPLC; Perfil fenólico; Suco de uva; Vale do São Francisco. |
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Thesagro: |
Suco; Uva; Viticultura. |
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Thesaurus NAL: |
Grapes. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/89541/1/Mariana-Pereira-et-al-MJ-092013.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/95257/1/Mariana-Pereira-et-al-MJ-092013.pdf
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Marc: |
LEADER 02441naa a2200277 a 4500
001 1966365
005 2017-04-25
008 2013 bl uuuu u00u1 u #d
100 1 $aNATIVIDADE, M. M. P.
245 $aSimultaneous analysis of 25 phenolic compounds in grape juice for HPLC$bMethod validation and characterization of São Francisco Valley samples.$h[electronic resource]
260 $c2013
520 $aA HPLCmethod for simultaneous determination of 25 phenolic compounds in grape juicewas optimized, validated and applied in the characterization of juices produced in São Francisco Valley (SFV), Brazil. The performance characteristics of the method were established by assays with standard solutions of phenolic compounds, spiked and unspiked samples. Linearity,matrix effects, trueness, precision, detection and quantification limits were evaluated. Linearity was demonstrated in the concentration ranges tested for all phenolic compounds. Significant matrix effects were not identified for the studied compounds.Mean recoveries ranged from86.18 to 106.50%, demonstrating no lack of trueness. Precision of the method was confirmed for the 25 phenolic compounds, with acceptable repeatability relative standard deviations (from 0.71 to 9.24%) and within-reproducibility relative standard deviations (from1.34 to 9.26%) for unspiked and spiked samples. The theoretical limits of detection and quantification of themethod varied from 0.001 to 0.19 Mg mL-1 and 0.003 to 0.37 Mg mL-1, respectively. The results of the validation process showed that the proposed method is fitness for purpose. This method was able to identify simultaneously 25 phenolic compounds and had advantages such as low consumption of solvents and easy sample preparation. The phenolic profile of the grape juices from SFV varied according the grape cultivar. Phenolics of the anthocyanins and tannins class predominated in red grape juices, while in white grape juice phenolic acids and tannins were found at high concentrations.
650 $aGrapes
650 $aSuco
650 $aUva
650 $aViticultura
653 $aComposto fenológico
653 $aHPLC
653 $aPerfil fenólico
653 $aSuco de uva
653 $aVale do São Francisco
700 1 $aCORREA, L. C.
700 1 $aSOUZA, S. V. C. de
700 1 $aPEREIRA, G. E.
700 1 $aLIMA, L. C. de O.
773 $tMicrochemical Journal, New York$gv. 110, p. 665-674, abr. 2013.
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Embrapa Semiárido (CPATSA) |
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