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| Registros recuperados : 7 | |
| 2. |  | ZAGATTO, E. A. G.; BERGAMIN FILHO, H.; BRIENZA, S. M. B.; ARRUDA, M. A. Z.; NOGUEIRA, A. R. de A.; LIMA, J. L. F. C. Multi-site detection in flow analysis: part 1. Relocation of spectrophotometric detector. Analytica Chimica Acta, Amsterdam, v.261, p.59-65, 1992.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | SILVA, F. V.; NOGUEIRA, A. R. de A.; SOUZA, G. B. de; REIS, B. F.; MONTENEGRO, M. C. M. S.; LIMA, J. L. F. C. Utilização do feijão de porco (Canavalia ensiformis DC) na determinação potenciométrica de uréia em leite por injeção seqüencial. In: SIMPÓSIO BRASILEIRO DE ELETROQUÍMICA E ELETROANALÍTICA, 9., 1999, Maragori, AL. Anais... Maragori: XI SIBEE, 1999. p.484-486.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | SILVA, F. V.; NOGUEIRA, A. R. de A.; SOUZA, G. B. de; REIS, B. F.; ARAÚJO, A. N.; MONTENEGRO, M. C. M. B. S.; LIMA, J. L. F. C. Potentiometric determination of urea by sequential injection using Jack bean meal crude extract as a source of urease. Talanta, v.53, p.331-336, 2000.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 7 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
28/08/2000 |
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Data da última atualização: |
04/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
|
Autoria: |
SILVA, F. V.; NOGUEIRA, A. R. de A.; SOUZA, G. B. de; REIS, B. F.; ARAÚJO, A. N.; MONTENEGRO, M. C. M. B. S.; LIMA, J. L. F. C. |
|
Afiliação: |
FERNANDO V. SILVA, INSTITUTO DE QUÍMICA DE SÃO CARLOS; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; GILBERTO BATISTA DE SOUZA, CPPSE; BOAVENTURA F. REIS, CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA; ALBERTO N. ARAÚJO, CEQUP; MARIA C. M. B. S. MONTENEGRO, CEQUP; JOSÉ L. F. C. LIMA, CEQUP. |
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Título: |
Potentiometric determination of urea by sequential injection using Jack bean meal crude extract as a source of urease. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Talanta, v.53, p.331-336, 2000. |
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Idioma: |
Português |
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Conteúdo: |
A sequential injection system was proposed to accomplish the potentiometric determination of urea. This procedure used an ammonium tubular selective electrode to assess ammonium concentration produced by enzymatic hydrolysis of urea from Jack bean meal (Canar;alia ensiformis DC) crude extract. A gaseous diffusion device was coupled to the flow set-up allowing on-line sampling and suitable selectivity for determinations. A detection limit of 6.0 x 10 mol urea 1 ', a relative standard deviation of' 1.9% (n = 10) and a sampling rate of 20 samples h-1 ' were observed when 172 Sumner units (SU) of urease and 900 ul of sample were used. Results agreeing with a comparative method were obtained by the proposed procedure and the use of the crude extract solution combined with the sequential injection approach improved the performance, producing reproducible results and low costs in comparison with procedures using commercial enzymes. |
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Palavras-Chave: |
Ammonium tubular; Crude extract; Selective electrode; SIA. |
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Thesaurus NAL: |
urea. |
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Categoria do assunto: |
W Química e Física |
|
Marc: |
LEADER 01679naa a2200253 a 4500
001 1045118
005 2022-01-04
008 2000 bl uuuu u00u1 u #d
100 1 $aSILVA, F. V.
245 $aPotentiometric determination of urea by sequential injection using Jack bean meal crude extract as a source of urease.$h[electronic resource]
260 $c2000
520 $aA sequential injection system was proposed to accomplish the potentiometric determination of urea. This procedure used an ammonium tubular selective electrode to assess ammonium concentration produced by enzymatic hydrolysis of urea from Jack bean meal (Canar;alia ensiformis DC) crude extract. A gaseous diffusion device was coupled to the flow set-up allowing on-line sampling and suitable selectivity for determinations. A detection limit of 6.0 x 10 mol urea 1 ', a relative standard deviation of' 1.9% (n = 10) and a sampling rate of 20 samples h-1 ' were observed when 172 Sumner units (SU) of urease and 900 ul of sample were used. Results agreeing with a comparative method were obtained by the proposed procedure and the use of the crude extract solution combined with the sequential injection approach improved the performance, producing reproducible results and low costs in comparison with procedures using commercial enzymes.
650 $aurea
653 $aAmmonium tubular
653 $aCrude extract
653 $aSelective electrode
653 $aSIA
700 1 $aNOGUEIRA, A. R. de A.
700 1 $aSOUZA, G. B. de
700 1 $aREIS, B. F.
700 1 $aARAÚJO, A. N.
700 1 $aMONTENEGRO, M. C. M. B. S.
700 1 $aLIMA, J. L. F. C.
773 $tTalanta$gv.53, p.331-336, 2000.
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