Data/hora: 01/06/2024 06:35:49

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 2
Data corrente: | 22/02/2010
Data da última atualização: | 20/09/2010
Autoria: | CAMARÇO, R. F. E. A.; NASCIMENTO, A. K. Q. do; ANDRADE, E. C. de; LIMA, J. A. A.
Afiliação: | Rosa Felicia E. Araújo Camarço, UFC; Aline K. Queiroz do Nascimento, UFC; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; José Albersio A. Lima, UFC.
Título: | Biological, serological and molecular comparison between isolates of Cowpea severe mosaic virus.
Ano de publicação: | 2009
Fonte/Imprenta: | Tropical Plant Pathology, Brasília, DF, v. 34, n. 4, p. 239-244, jul./ago. 2009.
ISSN: | 1982-5676
Idioma: | Inglês

Conteúdo:

Cowpea, Vigna unguiculata, can be affected by several diseases, and those caused by viruses are considered of great importance. Cowpea severe mosaic virus (CPSMV), which belongs to the family Comoviridae, genus Comovirus, stands out for its severity and degree of incidence. Its genetic variability can give rise to different strains around the world, including in Brazil. The objective of the present research was to establish a biological, serological and molecular comparison between five CPSMV isolates obtained from naturally infected plants in different States of the Northeast of Brazil. Isolates were obtained from cowpea: CPSMV-AL (State of Alagoas), CPSMV- CE (Ceará); CPSMV-MC (Piauí), CPSMV-PE (Pernambuco) and an isolate obtained from Crotalaria paulinea, in the State of Maranhão - CPSMV-CROT. A host range study evidenced biological differences among the virus isolates, especially in cowpea genotypes. The isolates were showed to be serologically and molecularly related by polymerase chain reaction – PCR, using degenerated primers which amplified two conserved regions in the coat protein and in the replicase genes. Cloning and sequence of CPSMV-PE made possible its comparison with other CPSMV isolates and other virus species from the genus Comovirus.
Keywords: Vigna unguiculata, Comovirus, CPSMV, molecular differentiation.

Palavras-Chave: | CPSMV; Molecular differentiation.
Thesagro: | Vigna Unguiculata.
Thesaurus Nal: | Comovirus.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (26600-1) - UPC - AP - PP -  -  -  - PCT