Data/hora: 10/06/2024 17:18:43

Biblioteca(s): | Embrapa Suínos e Aves.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 28/09/2018
Data da última atualização: | 28/09/2018
Autoria: | GRESSLER, V.; FEDDERN, V.; PEIXOTO, J. de O.; LEDUR, M. C.; DALLA COSTA, O. A.; LIMA, G. J. M. M. de.
Afiliação: | VANESSA GRESSLER, CNPSA; VIVIAN FEDDERN, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; OSMAR ANTONIO DALLA COSTA, CNPSA; GUSTAVO JULIO MELLO M DE LIMA, CNPSA.
Título: | Application of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork.
Ano de publicação: | 2018
Fonte/Imprenta: | Journal of Food Protection, v. 81, n.8, p. 1258-1263, 2018.
DOI: | 10.4315/0362-028X.JFP-17-526
Idioma: | Inglês
Conteúdo: | A new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels.
Palavras-Chave: | B-agonista; Carne suína; Method validation; Método de validação; Músculo suíno; Ractopamina; Swine muscle.
Thesagro: | Carcaça; Cromatografia; Digestão; Espectrometria; Hidrolise; Nutrição Animal; Suíno.
Thesaurus Nal: | Animal nutrition; Digestion; Hydrolysis; Liquid chromatography; Mass spectrometry; Methodology; Ractopamine; Swine.
Categoria do assunto: | --
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (21463-1) - UPC - AP - DD -  -  -  - DIS