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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
04/09/2015 |
Data da última atualização: |
16/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FONSECA, F. C. de A.; FIRMINO, A. A. P.; MACEDO. L. L. P. de; COELHO, R. R.; SOUSA JÚNIOR, J. D. A. de; SILVA JUNIOR, O. B.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; GÓIS, L. A. B. de; SILVA, M. C. M. da; SA, M. F. G. de. |
Afiliação: |
FERNANDO CAMPOS DE ASSIS FONSECA, UnB; ALEXANDRE AUGUSTO PEREIRA FIRMINO, UnB; LEONARDO LIMA PEPINO DE MACEDO, UnB; ROBERTA RAMOS COELHO; JOSÉ DIJAIR ANTONINO DE SOUSA JÚNIOR, UnB; ORZENIL BONFIM SILVA-JUNIOR, UnB; ROBERTO COITI TOGAWA, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, Unb; LUIZ AVELAR BRANDÃO DE GÓIS, USINA TRUNFO, ALAGOAS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
Sugarcane giant borer transcriptome analysis and identification of genes related to digestion. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, fev., 2015. |
DOI: |
10.1371/journal.pone.0118231 |
Idioma: |
Inglês |
Conteúdo: |
Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. MenosSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST d... Mostrar Tudo |
Thesagro: |
Broca Gigante; Controle Biológico; Praga. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129096/1/journal.pone.0118231.pdf
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Marc: |
LEADER 02579naa a2200289 a 4500 001 2023363 005 2023-03-16 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0118231$2DOI 100 1 $aFONSECA, F. C. de A. 245 $aSugarcane giant borer transcriptome analysis and identification of genes related to digestion.$h[electronic resource] 260 $c2015 520 $aSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. 650 $aBroca Gigante 650 $aControle Biológico 650 $aPraga 700 1 $aFIRMINO, A. A. P. 700 1 $aMACEDO. L. L. P. de 700 1 $aCOELHO, R. R. 700 1 $aSOUSA JÚNIOR, J. D. A. de 700 1 $aSILVA JUNIOR, O. B. 700 1 $aTOGAWA, R. C. 700 1 $aPAPPAS JUNIOR, G. J. 700 1 $aGÓIS, L. A. B. de 700 1 $aSILVA, M. C. M. da 700 1 $aSA, M. F. G. de 773 $tPlos One, fev., 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
20/12/2023 |
Data da última atualização: |
21/12/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ARAUJO FILHO, J. C. de; BARROS, A. H. C.; LIMA, E. de P.; XAVIER, J. P. de S.; OLIVEIRA, F. C. S. F. de; SILVA FILHO, A. D. da; BARROS, J. P. F. G. |
Afiliação: |
JOSE COELHO DE ARAUJO FILHO, CNPS; ALEXANDRE HUGO CEZAR BARROS, CNPS; EVALDO DE PAIVA LIMA, CNPS; JOAQUIM PEDRO DE SANTANA XAVIER, UNIVERSIDADE FEDERAL DE PERNAMBUCO; FLAVIO CRISTIANO SALDANHA FERREIRA DE OLIVEIRA, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; ANTONIO DIOMESCIO DA SILVA FILHO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; JULIANA PATRÍCIA FERNANDES GUEDES BARROS, UNIVERSIDADE FEDERAL DE PERNAMBUCO. |
Título: |
Avaliação, predição e mapeamento de água disponível em solos do Brasil. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: CONGRESSO LATINO-AMERICANO DE CIÊNCIA DO SOLO, 23.; CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 38., 2023, Florianópolis. Anais [...]. Florianópolis: Epagri, 2023. p. 383. Ref. ID 145. |
Idioma: |
Português |
Conteúdo: |
Os principais objetivos deste trabalho foram: (1) organizar uma base de dados espaciais com informações de AD (mm/cm) considerando 10 grupamentos texturais de perfis de solos representativos do Brasil; (2) disponibilizar um catálogo com valores de AD organizados em acordo com as classes taxonômicas vigentes no Sistema Brasileiro de Classificação de Solos (SiBCS); (3) elaborar e avaliar funções de pedotransferência (PTFs) para predição de AD; (4) desenvolver um protocolo com procedimentos para elaboração de mapas de AD a partir de mapas pedológicos em qualquer escala; e (5) elaborar um mapa de AD de solos do Brasil. |
Palavras-Chave: |
Mapeamento de Água Disponível; Modelagem (PTF). |
Thesagro: |
Água do Solo; Disponibilidade de Água. |
Thesaurus NAL: |
Soil water. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160149/1/Avaliacao-predicao-e-mapeamento-de-agua-disponivel-2023.pdf
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Marc: |
LEADER 01536nam a2200241 a 4500 001 2160149 005 2023-12-21 008 2023 bl uuuu u00u1 u #d 100 1 $aARAUJO FILHO, J. C. de 245 $aAvaliação, predição e mapeamento de água disponível em solos do Brasil.$h[electronic resource] 260 $aIn: CONGRESSO LATINO-AMERICANO DE CIÊNCIA DO SOLO, 23.; CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 38., 2023, Florianópolis. Anais [...]. Florianópolis: Epagri, 2023. p. 383. Ref. ID 145.$c2023 520 $aOs principais objetivos deste trabalho foram: (1) organizar uma base de dados espaciais com informações de AD (mm/cm) considerando 10 grupamentos texturais de perfis de solos representativos do Brasil; (2) disponibilizar um catálogo com valores de AD organizados em acordo com as classes taxonômicas vigentes no Sistema Brasileiro de Classificação de Solos (SiBCS); (3) elaborar e avaliar funções de pedotransferência (PTFs) para predição de AD; (4) desenvolver um protocolo com procedimentos para elaboração de mapas de AD a partir de mapas pedológicos em qualquer escala; e (5) elaborar um mapa de AD de solos do Brasil. 650 $aSoil water 650 $aÁgua do Solo 650 $aDisponibilidade de Água 653 $aMapeamento de Água Disponível 653 $aModelagem (PTF) 700 1 $aBARROS, A. H. C. 700 1 $aLIMA, E. de P. 700 1 $aXAVIER, J. P. de S. 700 1 $aOLIVEIRA, F. C. S. F. de 700 1 $aSILVA FILHO, A. D. da 700 1 $aBARROS, J. P. F. G.
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Embrapa Solos (CNPS) |
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