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| Registros recuperados : 70 | |
| 8. |  | LIMA, A. O. de S.; SCHWARTZ, G.; SILVA, A. R.; LUNZ, A. M.; MELLO, A. H. de; ALBINO, U. B. Efeito de microrganismos indutores de crescimento e adubação química em paricá em solo arenoso. Brazilian Journal of Development, v. 6, n. 11, p. 91670-91683, nov. 2020.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | ALBUQUERQUE, T. C. S. de; LIMA, H. E. de; LIMA, A. O.; SILVA, L. M.; SILVA, P. L. Absorção de nitrogênio em acessos de cupuaçuzeiro com diferentes níveis de tolerância a vassoura-de-bruxa em Roraima. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 25., 2017; REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DE HORTICULTURA TROPICAL, 62., 2017. Resumos... Porto Seguro, BA: SIHT, 2017.| Biblioteca(s): Embrapa Roraima. |
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| 10. | | LIMA, A. O. de S.; SCHWARTZ, G.; SILVA, A. R.; LUNZ, A. M.; MELLO, A. H. de; ALBINO, U. B. Adaptabilidade de clones de eucalipto à inoculação de fungos micorrízicos em solo arenoso em São Domingos do Araguaia - PA. Colloquium Agrariae, v. 15, n. 6, p. 1-10, nov./dez. 2019.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. Exploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | MENDES, R.; KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L. Metagenomic analysis of sheep rumen microbiome for carbohidrate-active genes discovery. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 16., 2016, Montreal. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2016. p. 836.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 13. | | OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; MELO, A. L. de; REGITANO, L. C. de A. Variantes da região promotora do gene KCNJ11 em bovinos da raça Nelore. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2018. p. 26. (Embrapa Instrumentação. Documentos, 68). Editores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | BORTOLON, L.; LIMA, A. O.; BORTOLON, E. S. O.; ROCHA, P. H. F.; CONCEIÇÃO, W. S. S.; SOUZA, J. P.; ALMEIDA, R. E. M.; CAMARGO, F. P. Spatial and temporal variation of near surface soil moisture and soil temperature in integrated crop-livestock system. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 15. | | LOPES, L. D.; SILVA, L. R. F.; ROMAGNOLI, E. M.; FERREIRA, C; TAKETANI, R. G.; LOUVANDINI, H; LIMA, A. O. S.; ABDALLA, A. L.; MENDES, R. Sheep rumen microbiome sequencing using Ion Torrent (PGM) platform. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY, 12., 2013, Ljubljana (Slovenia). Networking and plasticity of microbial communities: the secret to success. Ljubljana: University of Ljubljana. 2013. p. 68, ref. P31.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 16. | | LOPES, L. D.; LIMA, A. O. S.; SILVA, L. R. F.; ROMAGNOLI, E. M.; TAKETANI, R. G.; FERREIRA, C; ABDALLA, A. L.; MENDES, R. Identificação de enzimas lignocelulolíticas no microbioma do rúmen de ovinos usando metagenômica shotgun. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... Natal: Sociedade Brasileira de Microbiologia, 2013. Resumo 901-1| Biblioteca(s): Embrapa Meio Ambiente. |
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| 17. | | MOKRY, F. B.; LIMA, A. O. de; URBINATI, I.; TORRES JUNIOR, R. A. de A.; HIGA, R. H.; REGITANO, L. C. de A. Associação de SNPs com características de carcaça em uma população da raça Canchim. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 9., 2012, João Pessoa. Anais... João Pessoa: SBMA, 2012. Não paginado. SBMA 2012.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Gado de Corte; Embrapa Pecuária Sudeste. |
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| 18. | | SERRA, V.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LIMA, A. O. de; GASPARIN, G.; COUTINHO, L. L.; MOURÃO, G. B.; REGITANO, L. C. de A. Análise de qualidade de RNA para estudo do perfil transcriptômico de animais extremos para eficiência alimentar da raça Nelore. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 5., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação , 2013. p. 12 (Embrapa Pecuária Sudeste. Documentos, 110). Editado por Ana Rita de Araújo nogueira, simone Cristina Méo Nicura| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 19. | | OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; CARDOSO, T. F.; BRUSCADIN, J. J.; REGITANO, L. C. de A. Associação entre variantes cis regulatórias do gene KCNJ11e características econômicas em bovinos Nelore. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 11., 2019, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2019. p.23| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | MOKRY, F. B.; LIMA, A. O. de; MUDADU, M. A.; HIGA, R. H.; MEIRELLES, S. L. C.; REGITANO, L. C. de A. An insight into the linkage disequilibrium map of the Canchim beef cattle breed. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 8., 2012, Campinas. Abstract book... Ribeirão Preto: AB3C, 2012. Não paginado. X-MEETING 2012.| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 70 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
11/02/2016 |
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Data da última atualização: |
12/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KMIT, M. C. P.; LIMA, A. O. S.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. |
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Afiliação: |
M. C. P. KMIT, ESALQ/USP; A. O. S. LIMA, UNIVALI; E. M. ROMAGNOLI, ESALQ/USP; A. L. ABDALLA, CENA; RODRIGO MENDES, CNPMA. |
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Título: |
Sheep rumen shotgun sequencing for biomass-degrading genes discovery. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1676-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulases, 13 amylases and 3 other putative CAE from animals fed with control diet and 106 putative partial CAE representing a total of 52 lignocellulases, 46 amylases and 8 other putative CAE from animals fed with diet amended with sugarcane bagasse. These data sets shows the sheep rumen microbiome as an untapped source of potential new fibrolytic enzymes. Using a diet amended with sugarcane bagasse increases the abundance of CAE and provide a substantially expanded catalog of genes participating in the deconstruction of plant biomass MenosAbstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulas... Mostrar Tudo |
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Palavras-Chave: |
Lignocelluloytic enzymes; Microbial metagenome; Secind generation biofuel. |
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Thesagro: |
Biotecnologia; Enzima. |
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Thesaurus NAL: |
Metagenomics. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138802/1/2015RA-065.pdf
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|
Marc: |
LEADER 02806nam a2200229 a 4500
001 2036656
005 2016-02-12
008 2015 bl uuuu u00u1 u #d
100 1 $aKMIT, M. C. P.
245 $aSheep rumen shotgun sequencing for biomass-degrading genes discovery.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1676-1.$c1676
520 $aAbstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulases, 13 amylases and 3 other putative CAE from animals fed with control diet and 106 putative partial CAE representing a total of 52 lignocellulases, 46 amylases and 8 other putative CAE from animals fed with diet amended with sugarcane bagasse. These data sets shows the sheep rumen microbiome as an untapped source of potential new fibrolytic enzymes. Using a diet amended with sugarcane bagasse increases the abundance of CAE and provide a substantially expanded catalog of genes participating in the deconstruction of plant biomass
650 $aMetagenomics
650 $aBiotecnologia
650 $aEnzima
653 $aLignocelluloytic enzymes
653 $aMicrobial metagenome
653 $aSecind generation biofuel
700 1 $aLIMA, A. O. S.
700 1 $aROMAGNOLI, E. M.
700 1 $aABDALLA, A. L.
700 1 $aMENDES, R.
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