Data/hora: 20/06/2024 20:29:55

Biblioteca(s): | Embrapa Meio Ambiente.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 04/01/2016
Data da última atualização: | 10/03/2016
Autoria: | LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R.
Afiliação: | LUCAS DANTAS LOPES; ANDRE OLIVEIRA DE SOUZA LIMA, UNIVALI; RODRIGO GOUVEA TAKETANI; PHILLIP DARIAS, UNIVALI; LILIA RAQUEL FE DA SILVA, UFPI; EMILIANA MANESCO ROMAGNOLI; HELDER LOUVANDINI, CENA-USP; ADIBE LUIZ ABDALLA, CENA-USP; RODRIGO MENDES, CNPMA.
Título: | Exploring the sheep rumen microbiome for carbohydrate-active enzymes.
Ano de publicação: | 2015
Fonte/Imprenta: | Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015.
Idioma: | Inglês
Conteúdo: | Abstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes
Palavras-Chave: | Ion torrent (PGM).
Thesagro: | Amilase; Bactéria; Enzima; Ovelha; Rúmen.
Thesaurus Nal: | Amylases; Bacterial communities; Lignocellulose; Metagenomics; Phylogeny.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (14755-1) - UPC - AP - DD -  -  -  - DIS