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| Registros recuperados : 159 | |
| 81. |  | FROTA, P. C. E.; LIMA, A. A. C. L.; CRISOSTOMO, L. A.; AQUINO, A. R. L. de. Aspectos do solo, clima, fenologia e produção. In: ARAÚJO, J. P. P. de (Ed.). Caju: o produtor pergunta, a Embrapa responde. 2.ed. rev. e atualiz. Brasília, DF: Embrapa, 2015. Cap. 2 p. 33-40.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 83. | | CRISOSTOMO, L. A.; LIMA, A. A. C.; AQUINO, A. R. L. de; OLIVEIRA, F. N. S.; FROTA, P. C. E. Adubação e nutrição mineral. In: ARAÚJO, J. P. P. de (Ed.). Caju: o produtor pergunta, a Embrapa responde. 2.ed. rev. e atualiz. Brasília, DF: Embrapa, 2015. Cap. 3, p. 41-56.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 88. | | EMRICH, E. B.; SOUZA, R. J. de; LIMA, A. A. de; FIGUEIREDO, F. C.; SILVA, D. R. G. Cultivo do tomateiro em substrato orgânico sob aplicação foliar de silicato de potássio em ambiente protegido. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 56-61, jan./fev. 2011.| Biblioteca(s): Embrapa Hortaliças. |
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| 93. | | SÁGIO, S. A.; LIMA, A. A.; BARRETO, H. G.; CARVALHO, C. H. S. de; PAIVA, L. V.; CHALFUN-JUNIOR, A. Physiological and molecular analyses of early and late Coffea arabica cultivars at different stages of fruit ripening. Acta Physiologiae Plantarum, v. 35, n. 11, p. 3091-3098, 2014.| Biblioteca(s): Embrapa Café. |
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| 95. | | LIMA, E. A. de; COELHO, F. C.; LIMA, A. A. de; DORNELLES, M. S.; GARCIA, R. F. Semeadura direta de soja sobre diferentes plantas de cobertura e manejos de palhada, na região norte fluminense. Revista Ceres, Viçosa, MG, v. 56, n. 6, p. 790-795, nov./dez. 2009.| Biblioteca(s): Embrapa Florestas. |
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| 96. | | AZEREDO, G. A.; MATOS, V. P.; LIMA, A. A.; GONCALVES, E. P.; ADOLFO FILHO, J.; SILVA, A. Sementes de sapoti (Achras sapota L.): Germinacao e dormencia Curitiba, PR: IAPAR/SBF, 1996 p.386 In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 14. REUNIAO INTERAMERICANA DE HORTICULTURA TROPICAL, 42, 1996, Curitiba, PR.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 97. | | DANIELI, T.; LIMA, A. A. de; PELLEGRINO, A. C.; PINTO, A. de S.; PARRA, J. R. P.; CORRÊA-FERREIRA, B. S.; BONAGURIO, V. P. Alternância entre dietas natural e artificial entre as fases ninfal e adulta de Nezara viridula. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 1574-2.| Biblioteca(s): Embrapa Soja. |
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| 98. | | ALCANTARA, R. M. C. M. de; ARAÚJO, A. M. S. de; LIMA, A. A. de; HAIM, P. G.; SILVA, E. E. da. Avaliação da biomassa microbiana do solo em sistemas orgânicos. Revista Brasileira de Agroecologia, v. 2, n. 2, 2007. Edição dos Resumos do 5º Congresso Brasileiro de Agroecologia. Guarapari, ES, 2007.| Biblioteca(s): Embrapa Meio-Norte. |
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| 99. | | SCANDIUZZI, G. F.; LIMA, A. A. de; PELLEGRINO, A. C.; PINTO, A. de S.; PARRA, J. R. P.; CORRÊA-FERREIRA, B. S. Biologia de Nezara viridula criada por diferentes gerações em laboratório. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 2104-1.| Biblioteca(s): Embrapa Soja. |
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| 100. | | SANTOS, J. C. dos; GARCIA, M. V.; DUARTE, P. O.; OSHIRO, L. M.; MARTINS, F. I.; HIGA, L. de O. S.; LIMA, A. A. de; ANDREOTTI, R. Babesia bigemina (smith and Kilbourne, 1893) detection in Amblyomma sculptum (Berlese, 1888) ticks in the Mato Grosso do Sul state, Brazil. Parasitology International, v. 102, article 102912, 2024.| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 159 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
09/10/2013 |
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Data da última atualização: |
24/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LIMA, A. A.; ARAGÃO, C. W. S.; CASTRO, M. E. B. de; OLIVEIRA, J. V. de C.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M. |
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Afiliação: |
ANABELE AZEVEDO LIMA, UNB; CLARA WANDENKOLCK SILVA ARAGÃO, UNB; MARIA ELITA BATISTA DE CASTRO, CENARGEN; JULIANA VELASCO DE CASTRO OLIVEIRA, USP; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN MORAIS RIBEIRO, UNB. |
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Título: |
A recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
PLOSone, v. 8, n. 9, e74592, Sept. 2013. |
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DOI: |
10.1371/journal.pone.0074592 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest. MenosOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed hi... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/90688/1/journal.pone.0074592.pdf
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Marc: |
LEADER 02600naa a2200205 a 4500
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024 7 $a10.1371/journal.pone.0074592$2DOI
100 1 $aLIMA, A. A.
245 $aA recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity.$h[electronic resource]
260 $c2013
520 $aOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest.
650 $aSoja
700 1 $aARAGÃO, C. W. S.
700 1 $aCASTRO, M. E. B. de
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700 1 $aSOSA-GÓMEZ, D. R.
700 1 $aRIBEIRO, B. M.
773 $tPLOSone$gv. 8, n. 9, e74592, Sept. 2013.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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