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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/04/1995 |
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Data da última atualização: |
10/04/1995 |
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Autoria: |
OBA, K.; IWATSUKI, N.; URITANI, I.; ALVAREZ, A. M.; GARCIA, V. V. |
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Título: |
Polyphenol oxidase in banana fruit and buds. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Tokyo, Japan: Japan Scientific Societies Press, 1994. |
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Páginas: |
p.35-46 |
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ISBN: |
4-7622-7755-X |
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Idioma: |
Inglês |
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Notas: |
In: URITANI, I.; GARCIA, V. V.; MENDOZA, E. M. Postharvest Biochemistry of plant food-materials in the tropics. |
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Conteúdo: |
PPO is primarily responsible for the enzymic browning that occurs in banana fruits and buds. This enzyme was extracted from banana buds in the presence of Triton X-100, isoascorbate, and Polyclar AT. Two isozymes, I and II, were separated and partially purified by chromotagraphy on Butyl Toyopearl 650 and DEAE-cellulose. They exhibited different mobilities in PAGE and showed optimum pHs of 6.8 and 5.5, respectively. Both isozymes showed apparent Km values of 0.5 mM for the best substrate, dopamine, with substrate inhibitions at higher concentrations. Isozymes I and II were inhibited competitively by NaCl with Ki values of 140 mM and 40 mM, respectively. Both have a high heat stablitity similar to banana pulp PPO, with respectively 88 and 95% of their initial activity retained after l-hr incubation at 70 grauC. |
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Palavras-Chave: |
Enzimas; Polyphenol. |
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Thesagro: |
Oxidação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01514naa a2200241 a 4500
001 1644446
005 1995-04-10
008 1994 bl uuuu u00u1 u #d
020 $a4-7622-7755-X
100 1 $aOBA, K.
245 $aPolyphenol oxidase in banana fruit and buds.
260 $c1994
300 $ap.35-46
500 $aIn: URITANI, I.; GARCIA, V. V.; MENDOZA, E. M. Postharvest Biochemistry of plant food-materials in the tropics.
520 $aPPO is primarily responsible for the enzymic browning that occurs in banana fruits and buds. This enzyme was extracted from banana buds in the presence of Triton X-100, isoascorbate, and Polyclar AT. Two isozymes, I and II, were separated and partially purified by chromotagraphy on Butyl Toyopearl 650 and DEAE-cellulose. They exhibited different mobilities in PAGE and showed optimum pHs of 6.8 and 5.5, respectively. Both isozymes showed apparent Km values of 0.5 mM for the best substrate, dopamine, with substrate inhibitions at higher concentrations. Isozymes I and II were inhibited competitively by NaCl with Ki values of 140 mM and 40 mM, respectively. Both have a high heat stablitity similar to banana pulp PPO, with respectively 88 and 95% of their initial activity retained after l-hr incubation at 70 grauC.
650 $aOxidação
653 $aEnzimas
653 $aPolyphenol
700 1 $aIWATSUKI, N.
700 1 $aURITANI, I.
700 1 $aALVAREZ, A. M.
700 1 $aGARCIA, V. V.
773 $tTokyo, Japan: Japan Scientific Societies Press, 1994.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | LEON, C. de; NARRO, L. A.; ARIAS, M. P.; SALAZAR, F.; MORALES, F.; GERONIMO, L.; MACHADO, V.; PARENTONI, S.; RESENDE, I.; REYES, S.; GALVEZ, M.; CABRERA, S. Desarrollo de resistencia a plagas y enfermedades del maiz en America del Sur - Un proyecto colaborativo. In: REUNION LATINOAMERICANA DEL MAIZ, 18., 1999, Sete Lagoas. Memorias... Sete Lagoas: Embrapa Milho e Sorgo; Mexico: CIMMYT, 1999. p. 377-386.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 2. | | WELCKER, C.; THÉ, C.; ANDRÉAU, B.; LEON, C. de; PARENTONI, S. N.; BERNAL, J.; FÉLICITÉ, J.; ZONKENG, C.; SALAZAR, F.; NARRO, L.; CHARCOSSET, A.; HORST, W. J. Heterosis and combining ability for maize adaptation to tropical acid soils: implications for future breeding strategies. Crop Science, Madison, v. 42, n. 2, p. 2405-2413, 2005.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 2 | |
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