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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
24/06/2013 |
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Data da última atualização: |
07/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELABONA, P. da S.; PIROTA, R. D. P. B.; CODIMA, C. A.; TREMACOLDI, C. R.; RODRIGUES, A.; FARINAS, C. S. |
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Afiliação: |
PRISCILA DA SILVA DELABONA, CNPDIA/UFSCAR; ROSANGELA DONIZETE PERPETUA BUZON PIROTA, CNPDIA/UFSCAR; CARLA ALOIA CODIMA, CNPDIA/UFSCAR; CELIA REGINA TREMACOLDI, CPATU; ANDRÉ RODRIGUES, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Effect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues: Biomass-degrading enzymes production and characterization. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Industrial Crops and Products, v. 42, p. 236-242, 2013. |
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DOI: |
http://dx.doi.org/10.1016/j.indcrop.2012.05.035 |
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Idioma: |
Inglês |
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Conteúdo: |
Production of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in the acidic pH range, with maximal activities at the range of 50?65 °C, demonstrating the potential of the organism for the production of acidophilic and thermophilic biomass-degrading enzymes. MenosProduction of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in... Mostrar Tudo |
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Palavras-Chave: |
Resíduo agroindustrial. |
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Thesagro: |
Biomassa. |
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Thesaurus Nal: |
Amazonia. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02498naa a2200229 a 4500 001 1960431 005 2022-11-07 008 2013 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1016/j.indcrop.2012.05.035$2DOI 100 1 $aDELABONA, P. da S. 245 $aEffect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues$bBiomass-degrading enzymes production and characterization.$h[electronic resource] 260 $c2013 520 $aProduction of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in the acidic pH range, with maximal activities at the range of 50?65 °C, demonstrating the potential of the organism for the production of acidophilic and thermophilic biomass-degrading enzymes. 650 $aAmazonia 650 $aBiomassa 653 $aResíduo agroindustrial 700 1 $aPIROTA, R. D. P. B. 700 1 $aCODIMA, C. A. 700 1 $aTREMACOLDI, C. R. 700 1 $aRODRIGUES, A. 700 1 $aFARINAS, C. S. 773 $tIndustrial Crops and Products$gv. 42, p. 236-242, 2013.
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| 4. |  | CARPEJANI, G. C.; LEMPP, B.; MEDEIROS, S. R.; JANK, L.; GONÇALVES, M. C.; GOMES, R. A. Acúmulo de massa, composição química e digestibilidade in vitro em genótipos de Panicum maximum. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 2., 2006, Campo Grande, MS. Anais [da]... 2. ed. Campo Grande, MS: Embrapa Gado de Corte, 2006. 1 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. |  | LEMPP, B.; GOMES, R. A; VALLE, C. B. do; GALDEIA, E. C. Anatomia foliar e desaparecimento de tecidos de lâminas foliares de Brachiaria brizantha. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011. 1 CD-ROM. SBZ 2011. p. 1-3 1 CD-ROM Título em inglês: Leaf blead anatomy and tissue disappearance of Brachiaria brizantha. SBZ 2011| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. |  | LEMPP, B.; GOMES, R. A.; VALLE, C. B. do; SILVA, E. B. de A. Arranjo e proporção de tecidos em lâminas foliares de "Brachiaria" spp. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 41., 2004, Campo Grande, MS. A produção animal e a segurança alimentar: anais dos simpósios e dos resumos. Campo Grande, MS: Sociedade Brasileira de Zootecnia: Embrapa Gado de Corte, 2004. 4 p. 1 CD-ROM. FORR 073| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. |  | MAYER, L. R. R.; JANK, L.; LEMPP, B.; COSTA, R. de A. Seleção de genótipos de Panicum maximum (Megathyrsus maximus) para produção de bioenergia. In:SIMPÓSIO INTERNACIONAL SOBRE MELHORAMENTO DE FORRAGEIRAS, 2., 2009, Campo Grande, MS. [Anais]... Campo Grande, MS: Embrapa Gado de Corte, 2009. II SIMF. Comitê editorial: Liana Jank; Lucimara Chiari; Rosângela Maria Simeão Resende. 4 p. 1 CD-ROM. Resumo M 37.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. |  | GOMES, R. A.; LEMPP, B.; JANK, L.; CARPEJANI, G. C.; MORAIS, M. da G. Características anatômicas e morfofisiológicas de lâminas foliares de genótipos de Panicum maximum. Pesquisa Agropecuária Brasileira, Brasília, v.46, n.2, p.205-211, fev. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Unidades Centrais. |
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| Registros recuperados : 61 | |
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