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Registros recuperados : 47 | |
6. | | COSTA, M. R.; MORAES, F. E.; LEMOS, E. G. M.; MERCANTE, F. M. Caracterização molecular de isolados bacterianos de nódulos de soja cultivada sob diferentes manejos. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. Editado por Adilson de Oliveira Júnior, Clara Beatriz Hoffmann-Campo, Regina Maria Villas Bôas de Campos Leite, Ricardo Vilela Abdelnoor. 1 CD-ROM Trabalho publicado também nos Resumos do CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Biblioteca(s): Embrapa Agropecuária Oeste. |
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8. | | WICKERT, E.; LEMOS, E, G, M.; PEREIRA, F, M.; MAYER, A, N. Mercadores fAFLP na caracterização de três genótipos de umezeiro selecionados como porta-enxertos para pessegueiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 12, p. 1741-1746, dez. 2007. Biblioteca(s): Embrapa Clima Temperado. |
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9. | | HE, C. X.; CIAPINA, L. P.; DONADIO, L. C. LI, W. B.; LEMOS, E. G. M. Eric-pcr for early detection of bacterium (Xylella fastidiosa) of citrus variegated chlorosis Curitiba, PR: IAPAR/SBF, 1996 p.447 In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 14. REUNIAO INTERAMERICANA DE HORTICULTURA TROPICAL, 42, 1996, Curitiba, PR. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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13. | | GONELA, A.; LEMOS, E. G. M.; RODRIGUES, T. de J. D.; PATERNIANI, M. L. S. Reação enzimática ao estresse salino durante a germinação de estilosantes . Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 1, p. 93-95, jan. 2004 Notas Científicas.
Título em inglês: Enzymatic reaction to saline stress during stylosanthes germination. Biblioteca(s): Embrapa Unidades Centrais. |
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15. | | GRILLI, G. V. G.; BRAZ, L. R.; LEMOS, E. G. M. Uso dos marcadores moleculares FAFLP na identificação de QTL´S para tolerância ao calor na característica de fixação de frutos em tomateiro. Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 391, jul. 2004. Suplemento 1. Biblioteca(s): Embrapa Hortaliças. |
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16. | | ZACCARO, R. P.; CARARETO-ALVES, L. M.; TRAVENSOLO, R. F.; WICKERT, E.; LEMOS, E. G. M. Utilização de marcador molecular scar na identificação de Fusarium subglutinans, agente causal da malformação da mangueira. Revista Brasileira de Fruticultura, Jaboticabal, v. 29, n. 3, p. 563-570, dez. 2007. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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17. | | GRILLI, G. V. G.; BRAZ, L. T.; LEMOS, E. G. M.; SILVA, A. V. C. da; DALPIAN, T. Avaliação de metodologia de extração de DNA genômico em tomateiros para análise genética com marcadores RAPD. Horticultura Brasileira, Brasília, v. 20, n. 2, p. 377, jul. 2002. Resumo. Biblioteca(s): Embrapa Hortaliças. |
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18. | | NOZAKI, M. de H.; CAMARGO, M.; LEMOS, E. G. M; AUKAR, A. P. de A.; BARRETO, M. Caracterização molecular através da técnica fAFLP de isolados de Diaporthe citri. Summa Phytopathologica, Botucatu, SP, v. 32, n. 2, p. 147-150, Apr./June 2006. Biblioteca(s): Embrapa Agricultura Digital. |
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19. | | GOTTARDI, M. V. C.; TRAVENSOLO, R. de F.; CAMPANHARO, J. C.; SANTOS, J. M. dos; LEMOS, E. G. M. Inoculação cruzada da bactéria Xylella fastidiosa em plantas de laranjeira, videira, ameixeira e cafeeiro. Summa Phytopathologica, Botucatu, v. 30, n. 4, p. 488-493, Oct./Dec. 2004. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 47 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
18/04/2023 |
Data da última atualização: |
18/04/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LIMA, N. S. M.; GOMES-PEPE, E. S.; KOCK, F. V. C.; COLNAGO, L. A.; LEMOS, E. G. M. |
Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
Título: |
Dynamics of the role of LacMeta laccase in the complete degradation and detoxification of malachite green. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 39, 127, 2023. |
Páginas: |
1 - 13 |
DOI: |
https://doi.org/10.1007/s11274-023-03572-w |
Idioma: |
Inglês |
Conteúdo: |
Laccases highlight for xenobiotic bioremediation, as well as application in the fine chemical, textile, biofuel and food industries. In a previous work, we described the preliminary characterization of laccase LacMeta, a promising enzyme for the bioremediation of dyes, able to decolorization malachite green (MG), trypan blue, methylene blue. Here we demonstrate that LacMeta is indeed suitable for the complete degradation and detoxification of MG dye, not just for its discoloration, since some works show false positives due to the formation of colorless intermediates such as leucomalachite. The optimal pH and temperature parameters of LacMeta were 5.0 and 50 °C, respectively (MG as substrate). LacMeta was tolerant of up to 10 mmol L−1 EDTA (82%) and up to 5% (V/V) acetone (91%) and methanol (71%), while SDS promoted severe inhibition. For ions, a high tolerance to cobalt, zinc, manganese, and calcium (10 mmol L−1 ) was also observed (>90%). Even under high-salinity conditions (1 mol L−1 NaCl), the residual bleaching activity of the dye remained at 61%. Furthermore, the bleaching product of MG did not inhibit the germination of sorghum and tomato seeds and was inert to the vegetative structures of the germinated seedlings. Additionally, this treatment effectively reduced the cytotoxic effect of the dye on microorganisms (Escherichia coli and Azospirillum brasilense), which can be explained by H-NMR spectral analysis results since LacMeta completely degraded the peak signals corresponding to the aromatic rings in the dye, demonstrating extreme efficiency in the bioremediation of the xenobiotic at high concentrations (50 mg L−1 ). MenosLaccases highlight for xenobiotic bioremediation, as well as application in the fine chemical, textile, biofuel and food industries. In a previous work, we described the preliminary characterization of laccase LacMeta, a promising enzyme for the bioremediation of dyes, able to decolorization malachite green (MG), trypan blue, methylene blue. Here we demonstrate that LacMeta is indeed suitable for the complete degradation and detoxification of MG dye, not just for its discoloration, since some works show false positives due to the formation of colorless intermediates such as leucomalachite. The optimal pH and temperature parameters of LacMeta were 5.0 and 50 °C, respectively (MG as substrate). LacMeta was tolerant of up to 10 mmol L−1 EDTA (82%) and up to 5% (V/V) acetone (91%) and methanol (71%), while SDS promoted severe inhibition. For ions, a high tolerance to cobalt, zinc, manganese, and calcium (10 mmol L−1 ) was also observed (>90%). Even under high-salinity conditions (1 mol L−1 NaCl), the residual bleaching activity of the dye remained at 61%. Furthermore, the bleaching product of MG did not inhibit the germination of sorghum and tomato seeds and was inert to the vegetative structures of the germinated seedlings. Additionally, this treatment effectively reduced the cytotoxic effect of the dye on microorganisms (Escherichia coli and Azospirillum brasilense), which can be explained by H-NMR spectral analysis results since LacMeta completely degraded t... Mostrar Tudo |
Palavras-Chave: |
Detoxification; Halotolerance ·; Triphenylmethane dye. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02417naa a2200229 a 4500 001 2153222 005 2023-04-18 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s11274-023-03572-w$2DOI 100 1 $aLIMA, N. S. M. 245 $aDynamics of the role of LacMeta laccase in the complete degradation and detoxification of malachite green.$h[electronic resource] 260 $c2023 300 $a1 - 13 520 $aLaccases highlight for xenobiotic bioremediation, as well as application in the fine chemical, textile, biofuel and food industries. In a previous work, we described the preliminary characterization of laccase LacMeta, a promising enzyme for the bioremediation of dyes, able to decolorization malachite green (MG), trypan blue, methylene blue. Here we demonstrate that LacMeta is indeed suitable for the complete degradation and detoxification of MG dye, not just for its discoloration, since some works show false positives due to the formation of colorless intermediates such as leucomalachite. The optimal pH and temperature parameters of LacMeta were 5.0 and 50 °C, respectively (MG as substrate). LacMeta was tolerant of up to 10 mmol L−1 EDTA (82%) and up to 5% (V/V) acetone (91%) and methanol (71%), while SDS promoted severe inhibition. For ions, a high tolerance to cobalt, zinc, manganese, and calcium (10 mmol L−1 ) was also observed (>90%). Even under high-salinity conditions (1 mol L−1 NaCl), the residual bleaching activity of the dye remained at 61%. Furthermore, the bleaching product of MG did not inhibit the germination of sorghum and tomato seeds and was inert to the vegetative structures of the germinated seedlings. Additionally, this treatment effectively reduced the cytotoxic effect of the dye on microorganisms (Escherichia coli and Azospirillum brasilense), which can be explained by H-NMR spectral analysis results since LacMeta completely degraded the peak signals corresponding to the aromatic rings in the dye, demonstrating extreme efficiency in the bioremediation of the xenobiotic at high concentrations (50 mg L−1 ). 653 $aDetoxification 653 $aHalotolerance · 653 $aTriphenylmethane dye 700 1 $aGOMES-PEPE, E. S. 700 1 $aKOCK, F. V. C. 700 1 $aCOLNAGO, L. A. 700 1 $aLEMOS, E. G. M. 773 $tWorld Journal of Microbiology and Biotechnology$gv. 39, 127, 2023.
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