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| Registros recuperados : 47 | |
| 5. |  | COSTA, M. R.; MORAES, F. E.; LEMOS, E. G. M.; MERCANTE, F. M. Caracterização molecular de isolados bacterianos de nódulos de soja cultivada sob diferentes manejos. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. Editado por Adilson de Oliveira Júnior, Clara Beatriz Hoffmann-Campo, Regina Maria Villas Bôas de Campos Leite, Ricardo Vilela Abdelnoor. 1 CD-ROM Trabalho publicado também nos Resumos do CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 9. | | HE, C. X.; CIAPINA, L. P.; DONADIO, L. C. LI, W. B.; LEMOS, E. G. M. Eric-pcr for early detection of bacterium (Xylella fastidiosa) of citrus variegated chlorosis Curitiba, PR: IAPAR/SBF, 1996 p.447 In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 14. REUNIAO INTERAMERICANA DE HORTICULTURA TROPICAL, 42, 1996, Curitiba, PR.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | WICKERT, E.; LEMOS, E, G, M.; PEREIRA, F, M.; MAYER, A, N. Mercadores fAFLP na caracterização de três genótipos de umezeiro selecionados como porta-enxertos para pessegueiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 12, p. 1741-1746, dez. 2007.| Biblioteca(s): Embrapa Clima Temperado. |
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| 13. | | GONELA, A.; LEMOS, E. G. M.; RODRIGUES, T. de J. D.; PATERNIANI, M. L. S. Reação enzimática ao estresse salino durante a germinação de estilosantes . Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 1, p. 93-95, jan. 2004 Notas Científicas.
Título em inglês: Enzymatic reaction to saline stress during stylosanthes germination.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 15. | | GRILLI, G. V. G.; BRAZ, L. R.; LEMOS, E. G. M. Uso dos marcadores moleculares FAFLP na identificação de QTL´S para tolerância ao calor na característica de fixação de frutos em tomateiro. Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 391, jul. 2004. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | ZACCARO, R. P.; CARARETO-ALVES, L. M.; TRAVENSOLO, R. F.; WICKERT, E.; LEMOS, E. G. M. Utilização de marcador molecular scar na identificação de Fusarium subglutinans, agente causal da malformação da mangueira. Revista Brasileira de Fruticultura, Jaboticabal, v. 29, n. 3, p. 563-570, dez. 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 17. | | NOZAKI, M. de H.; CAMARGO, M.; LEMOS, E. G. M; AUKAR, A. P. de A.; BARRETO, M. Caracterização molecular através da técnica fAFLP de isolados de Diaporthe citri. Summa Phytopathologica, Botucatu, SP, v. 32, n. 2, p. 147-150, Apr./June 2006.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 18. | | GRILLI, G. V. G.; BRAZ, L. T.; LEMOS, E. G. M.; SILVA, A. V. C. da; DALPIAN, T. Avaliação de metodologia de extração de DNA genômico em tomateiros para análise genética com marcadores RAPD. Horticultura Brasileira, Brasília, v. 20, n. 2, p. 377, jul. 2002. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 20. | | GOTTARDI, M. V. C.; TRAVENSOLO, R. de F.; CAMPANHARO, J. C.; SANTOS, J. M. dos; LEMOS, E. G. M. Inoculação cruzada da bactéria Xylella fastidiosa em plantas de laranjeira, videira, ameixeira e cafeeiro. Summa Phytopathologica, Botucatu, v. 30, n. 4, p. 488-493, Oct./Dec. 2004.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 47 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2012 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FEDERICI, M. T.; PICCHI, S. C.; STUCHI, E. S.; FADEL, A. L.; LAIA, M. L.; LEMOS, M. V. F.; LEMOS, E. G. M. |
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Afiliação: |
MARÍA TERESA FEDERICI, INIA; SIMONE CRISTINA PICCHI, UNESP; EDUARDO SANCHES STUCHI, CNPMF; ANDRÉ LUIZ FADEL, ESALQ; MARCELO LUIZ LAIA, UFVJM; MANOEL VICTOR FRANCO LEMOS, UNESP; ELIANA GERTRUDES MACEDO LEMOS, UNESP. |
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Título: |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, 2012. |
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ISSN: |
0717-3458 |
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DOI: |
10.2225/vol15-issue3-fulltext-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increas... Mostrar Tudo |
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Palavras-Chave: |
Microarray. |
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Thesagro: |
Xylella Fastidiosa. |
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Thesaurus NAL: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
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260 $c2012
520 $aXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCitrus
650 $aXylella Fastidiosa
653 $aMicroarray
700 1 $aPICCHI, S. C.
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700 1 $aLAIA, M. L.
700 1 $aLEMOS, M. V. F.
700 1 $aLEMOS, E. G. M.
773 $tElectronic Journal of Biotechnology, 2012.
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