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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/11/2002 |
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Data da última atualização: |
06/11/2002 |
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Autoria: |
SEGERSON, E. C.; LI, H.; TALBOTT, C. W.; ALLEN, J. W.; GUNSETT, F. C. |
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Título: |
Partial characterization of ovine intrauterine suppressor cells. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 58, n. 2, p. 397-406, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Ovine uterine cells that represented Day 14 cyclic and pregnant endometrium were fractionated with Percoll and evaluated for suppression of cocultured phytohemagglutinin (PHA)-induced peripheral blood Iymphocyte (PBL) proliferation and for the presence of T-lymphocyte markers. Uterine cells were then evaluated for suppressor activity following the depletion of conventional lymphocyte classes (i.e., T-, B-, and NK-like) with complement + antibody treatment. In addition, supernatant (derived from cultured uterine cells) was tested for transforming growth factor Beta (TGFBeta) activity using neutralization antibodies to TGFBeta. Fractionated uterine cells (density range of 1.002-1.056 g/ml) from cyclic and pregnant ewes suppressed PHA-induced proliferation of PBL, and the majority (69.5%) of these cells were <= 5.2 um in diameter. Percentages of CD5+, CD4+, and CD8+ lymphocytes recovered from endometrial curettage were less for cells in this density range than for cells with greater densities. Uterine cells released suppressor factor(s) into the culture medium (supernatant); however, suppressor activity was unaffected by either anti. TGFBeta or complement + antibody treatment. In conclusion, low-density uterine cells from Day 14 cyclic and pregnant ewes suppressed the proliferation of cocultured PBL and released a suppressor factor(s) into the medium that did not exhibit TGFBeta activity. It is unlikely that the suppressor cells comprise conventional T-, B-, or NK-like lymphocyte lineages. MenosOvine uterine cells that represented Day 14 cyclic and pregnant endometrium were fractionated with Percoll and evaluated for suppression of cocultured phytohemagglutinin (PHA)-induced peripheral blood Iymphocyte (PBL) proliferation and for the presence of T-lymphocyte markers. Uterine cells were then evaluated for suppressor activity following the depletion of conventional lymphocyte classes (i.e., T-, B-, and NK-like) with complement + antibody treatment. In addition, supernatant (derived from cultured uterine cells) was tested for transforming growth factor Beta (TGFBeta) activity using neutralization antibodies to TGFBeta. Fractionated uterine cells (density range of 1.002-1.056 g/ml) from cyclic and pregnant ewes suppressed PHA-induced proliferation of PBL, and the majority (69.5%) of these cells were <= 5.2 um in diameter. Percentages of CD5+, CD4+, and CD8+ lymphocytes recovered from endometrial curettage were less for cells in this density range than for cells with greater densities. Uterine cells released suppressor factor(s) into the culture medium (supernatant); however, suppressor activity was unaffected by either anti. TGFBeta or complement + antibody treatment. In conclusion, low-density uterine cells from Day 14 cyclic and pregnant ewes suppressed the proliferation of cocultured PBL and released a suppressor factor(s) into the medium that did not exhibit TGFBeta activity. It is unlikely that the suppressor cells comprise conventional T-, B-, or NK-like lymphocy... Mostrar Tudo |
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Palavras-Chave: |
Imunosupressão. |
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Thesagro: |
Ovino; Reprodução Animal; Útero. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02085naa a2200217 a 4500
001 1528815
005 2002-11-06
008 1998 bl uuuu u00u1 u #d
100 1 $aSEGERSON, E. C.
245 $aPartial characterization of ovine intrauterine suppressor cells.
260 $c1998
520 $aOvine uterine cells that represented Day 14 cyclic and pregnant endometrium were fractionated with Percoll and evaluated for suppression of cocultured phytohemagglutinin (PHA)-induced peripheral blood Iymphocyte (PBL) proliferation and for the presence of T-lymphocyte markers. Uterine cells were then evaluated for suppressor activity following the depletion of conventional lymphocyte classes (i.e., T-, B-, and NK-like) with complement + antibody treatment. In addition, supernatant (derived from cultured uterine cells) was tested for transforming growth factor Beta (TGFBeta) activity using neutralization antibodies to TGFBeta. Fractionated uterine cells (density range of 1.002-1.056 g/ml) from cyclic and pregnant ewes suppressed PHA-induced proliferation of PBL, and the majority (69.5%) of these cells were <= 5.2 um in diameter. Percentages of CD5+, CD4+, and CD8+ lymphocytes recovered from endometrial curettage were less for cells in this density range than for cells with greater densities. Uterine cells released suppressor factor(s) into the culture medium (supernatant); however, suppressor activity was unaffected by either anti. TGFBeta or complement + antibody treatment. In conclusion, low-density uterine cells from Day 14 cyclic and pregnant ewes suppressed the proliferation of cocultured PBL and released a suppressor factor(s) into the medium that did not exhibit TGFBeta activity. It is unlikely that the suppressor cells comprise conventional T-, B-, or NK-like lymphocyte lineages.
650 $aOvino
650 $aReprodução Animal
650 $aÚtero
653 $aImunosupressão
700 1 $aLI, H.
700 1 $aTALBOTT, C. W.
700 1 $aALLEN, J. W.
700 1 $aGUNSETT, F. C.
773 $tBiology of Reproduction$gv. 58, n. 2, p. 397-406, 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
13/04/2015 |
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Data da última atualização: |
24/01/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MEDINA, B. F.; LEITE, J. A. |
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Afiliação: |
BENJAMIN FERNANDEZ MEDINA, IICA; JOSÉ AMÉRICO LEITE, CNPSD. |
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Título: |
Precipitation probabilities in Boa Vista - RR. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
In: SEMINÁRIO NACIONAL DA SERINGUEIRA, 4., 1984, Salvador. Resumo dos trabalhos. [S.l.]: SUDHEVEA/EMBRAPA/EMBRATER/Banco Econômico, [1984]. p. 215. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Precipitação; Roraima. |
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Thesagro: |
Chuva. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191276/1/R633.8952-S471r-pg-215.pdf
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Marc: |
LEADER 00493nam a2200145 a 4500
001 2013411
005 2019-01-24
008 1984 bl uuuu u00u1 u #d
100 1 $aMEDINA, B. F.
245 $aPrecipitation probabilities in Boa Vista - RR.
260 $aIn: SEMINÁRIO NACIONAL DA SERINGUEIRA, 4., 1984, Salvador. Resumo dos trabalhos. [S.l.]: SUDHEVEA/EMBRAPA/EMBRATER/Banco Econômico, [1984]. p. 215.$c1984
650 $aChuva
653 $aPrecipitação
653 $aRoraima
700 1 $aLEITE, J. A.
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Embrapa Amazônia Ocidental (CPAA) |
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