|
|
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
02/08/2016 |
Data da última atualização: |
28/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIOL, M. A.; GUERRERO, F. D.; OLIVEIRA, B. C. M. de; AQUINO, M. C. C. de; LOIOLA, S. H.; MELO, G. D. de; GOMES, A. H. de S.; KANAMURA, C. T.; GARCIA, M. V.; ANDREOTTI, R.; LIMA, V. M. F. de; BRESCIANI, K. D. S. |
Afiliação: |
Milena Araúz Viol, UNESP; Felix D. Guerrero, UNESP; Bruno César Miranda de Oliveira, UNESP; Monally Conceição Costa de Aquino, UNESP; Saulo Hudson Loiola, UNESP; Guilherme Dias de Melo, UNESP; Aparecida Helena de Souza Gomes, Instituto Adolfo Lutz; Cristina Takami Kanamura, INSTITUTO ADOLFO LUTZ; Marcos Valério Garcia, FUNDECT; RENATO ANDREOTTI E SILVA, CNPGC; Valéria Marçal Félix de Lima, UNESP; Katia Denise Saraiva Bresciani, UNESP. |
Título: |
Identification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Parasitology Research, v. 115, n, 9, p. 3479-3484, sept. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Sand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tickinfested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil. |
Palavras-Chave: |
Ixodids. |
Thesagro: |
Cão; Carrapato; Leishmaniose. |
Thesaurus Nal: |
Dogs; Immunohistochemistry; Leishmaniasis; Quantitative polymerase chain reaction. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158208/1/Identification-of-Leishmania-spp.-promastigotes.pdf
|
Marc: |
LEADER 02476naa a2200349 a 4500 001 2050014 005 2017-03-28 008 2016 bl uuuu u00u1 u #d 100 1 $aVIOL, M. A. 245 $aIdentification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs.$h[electronic resource] 260 $c2016 520 $aSand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tickinfested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil. 650 $aDogs 650 $aImmunohistochemistry 650 $aLeishmaniasis 650 $aQuantitative polymerase chain reaction 650 $aCão 650 $aCarrapato 650 $aLeishmaniose 653 $aIxodids 700 1 $aGUERRERO, F. D. 700 1 $aOLIVEIRA, B. C. M. de 700 1 $aAQUINO, M. C. C. de 700 1 $aLOIOLA, S. H. 700 1 $aMELO, G. D. de 700 1 $aGOMES, A. H. de S. 700 1 $aKANAMURA, C. T. 700 1 $aGARCIA, M. V. 700 1 $aANDREOTTI, R. 700 1 $aLIMA, V. M. F. de 700 1 $aBRESCIANI, K. D. S. 773 $tParasitology Research$gv. 115, n, 9, p. 3479-3484, sept. 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
24/04/2015 |
Data da última atualização: |
24/04/2015 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ANGÉLICA, R. S.; LEITE, A. A. da S.; BRAGA, F. C. S.; OLIVEIRA, M. A. de; OLIVEIRA, K. S.; MARTINS, E. de S. |
Afiliação: |
EDER DE SOUZA MARTINS, CPAC. |
Título: |
X-ray powder diffraction analysis as a tool in mineral exploration for agromineral evaluation. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: WORLD FERTILIZER CONGRESS OF CIEC: TECHNOLOGICAL INNOVATION FOR A SUSTAINABLE TROPICAL AGRICULTURE, 16., Rio de Janeiro. Proceedings... Rio de Janeiro: CIEC, 2014. |
Páginas: |
p. 214-216 |
Idioma: |
Português |
Palavras-Chave: |
Agrominerais. |
Thesagro: |
Raio X. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00658naa a2200157 a 4500 001 2014132 005 2015-04-24 008 2014 bl uuuu u00u1 u #d 100 1 $aANGÉLICA, R. S.; LEITE, A. A. da S.; BRAGA, F. C. S.; OLIVEIRA, M. A. de; OLIVEIRA, K. S. 245 $aX-ray powder diffraction analysis as a tool in mineral exploration for agromineral evaluation. 260 $c2014 300 $ap. 214-216 650 $aRaio X 653 $aAgrominerais 700 1 $aMARTINS, E. de S. 773 $tIn: WORLD FERTILIZER CONGRESS OF CIEC: TECHNOLOGICAL INNOVATION FOR A SUSTAINABLE TROPICAL AGRICULTURE, 16., Rio de Janeiro. Proceedings... Rio de Janeiro: CIEC, 2014.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Cerrados (CPAC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|