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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
27/11/2015 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; CABRAL, E. C.; COELHO, M. B.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. |
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Afiliação: |
UNESP; UNESP; UNICAMP; UNICAMP; UNICAMP; UNICAMP; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP. |
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Título: |
Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Theriogenology, v. 84, n. 1, p. 127-136, 2015. |
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DOI: |
10.1016/j.theriogenology.2015.02.023 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species. MenosThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on... Mostrar Tudo |
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Palavras-Chave: |
Animal embryos; Bovine embryo; Cryosurvival; In vitro production; Lipid profile. |
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Thesagro: |
Bovino; Embrião animal; Reprodução animal. |
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Thesaurus Nal: |
Cattle; conjugated linoleic acid; In vitro fertilization; mass spectrometry. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02882naa a2200373 a 4500
001 2029790
005 2016-03-17
008 2015 bl --- 0-- u #d
024 7 $a10.1016/j.theriogenology.2015.02.023$2DOI
100 1 $aLEÃO, B. C. S.
245 $aImproved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile.
260 $c2015
520 $aThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species.
650 $aCattle
650 $aconjugated linoleic acid
650 $aIn vitro fertilization
650 $amass spectrometry
650 $aBovino
650 $aEmbrião animal
650 $aReprodução animal
653 $aAnimal embryos
653 $aBovine embryo
653 $aCryosurvival
653 $aIn vitro production
653 $aLipid profile
700 1 $aROCHA-FRIGONI, N. A. S.
700 1 $aCABRAL, E. C.
700 1 $aCOELHO, M. B.
700 1 $aFERREIRA, C. R.
700 1 $aEBERLIN, M. N.
700 1 $aACCORSI, M. F.
700 1 $aNOGUEIRA, E.
700 1 $aMINGOTI, G. Z.
773 $tTheriogenology$gv. 84, n. 1, p. 127-136, 2015.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/02/2012 |
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Data da última atualização: |
16/02/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, T. de J.; NOGUEIRA, M. A.; SANTOS, A. S.; LEDO, C. A. da S. |
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Afiliação: |
TAINÃ DE JESUS SANTOS, Colégio Estadual Lauro Passos; MILENA ANDRADE NOGUEIRA, Colégio Estadual Lauro Passos; ARIANA SILVA SANTOS, UFRB; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Polinização artifical entre espécies silvestres de Manihot e variedades de mandioca. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM, 2011. |
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Descrição Física: |
1 CD ROM. |
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Idioma: |
Português |
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Notas: |
Propagação. Resumo n. 112. |
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Conteúdo: |
A mandioca constitui um importante cultivo nos trópicos e os agricultores sempre estiveram envolvidos no seu processo de seleção e na conservação de variedades, desde os seus ancestrais remotos (Fukuda, 2006). O melhoramento da cultura da mandioca tem se desenvolvido em diversos estágios, ela se reproduz por propagação vegetativa, embora a produção de sementes sexuais ocorra facilmente nessa espécie, possui inúmeras vantagens em relação a outras culturas, tais como: facilidade de propagação, tolerância à seca e resistência/tolerância a pragas e doenças. |
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Palavras-Chave: |
Hibridação de mandioca; Melhoramento genético; Polonização de mandioca. |
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Thesagro: |
Mandioca. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54256/1/Polonizacao-artificial-Carlos-Ledo-Resumo-n.-112-Taina-Jesus-poster.PDF
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Marc: |
LEADER 01349nam a2200217 a 4500
001 1915852
005 2012-02-16
008 2011 bl uuuu u00u1 u #d
100 1 $aSANTOS, T. de J.
245 $aPolinização artifical entre espécies silvestres de Manihot e variedades de mandioca.
260 $aIn: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM$c2011
300 $c1 CD ROM.
500 $aPropagação. Resumo n. 112.
520 $aA mandioca constitui um importante cultivo nos trópicos e os agricultores sempre estiveram envolvidos no seu processo de seleção e na conservação de variedades, desde os seus ancestrais remotos (Fukuda, 2006). O melhoramento da cultura da mandioca tem se desenvolvido em diversos estágios, ela se reproduz por propagação vegetativa, embora a produção de sementes sexuais ocorra facilmente nessa espécie, possui inúmeras vantagens em relação a outras culturas, tais como: facilidade de propagação, tolerância à seca e resistência/tolerância a pragas e doenças.
650 $aMandioca
653 $aHibridação de mandioca
653 $aMelhoramento genético
653 $aPolonização de mandioca
700 1 $aNOGUEIRA, M. A.
700 1 $aSANTOS, A. S.
700 1 $aLEDO, C. A. da S.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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