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| Registros recuperados : 41 | |
| 15. |  | SILVA, J. de S.; CHEAZ, J.; SANTAMARIA, J.; MATO, M. A.; LEON, A. La dimensión de "estrategía" en la construcción de la sostenibilidad institucional. San José: ISNAR, 2001. 185 p. (Serie innovacion para la sostenibilidad institucional). Proyecto Nuevo Paradigma. Nome dos autores na capa: José de Souza Silva, Juan cheaz Peláez, Julio Santamaria guerra, Maria Adriana Mato Bode, Alicia León.| Biblioteca(s): Embrapa Acre; Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Cerrados; Embrapa Gado de Leite; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia; Embrapa Semiárido; Embrapa Solos; Embrapa Suínos e Aves. MenosEmbrapa Acre; Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Cerrados; Embrapa Gado de Leite; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte; Embrapa Pantanal... Mostrar Todas |
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| 20. | | LEON, A. J.; MOUTOUS, J.; MOUTOUS, M. C.; MANEY, M. M. ANDRADE, F. H. Efectos de la falta de polen en lineas machoesteriles de girasol (Helianthus annuus, L.) utilizadas en produccion de semilla hibrida. In: REUNION TECNICA NACIONAL DE GIRASOL, 5., 1987, Bahia Blanca. Actas... Bahia Blanca: ASAGIR / Universidad Nacional del Sur, [1987?]. p.231-236.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 41 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
05/08/2011 |
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Data da última atualização: |
05/08/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ANDREOTTI, R.; PÉREZ DE LEÓN, A. A.; DOWD, S. E.; GUERRERO, F. D.; BENDELE, K. G.; SCOLES, G. A. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; Adalberto A Pérez de León, 2USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., Kerrville, TX, 78028, USA; Scot E Dowd, 3Research and Testing Laboratory, Pathogenius, and Spirostat Technologies, 4321 Marsha Sharp Fwy., Lubbock, TX, 79407, USA; Felix D Guerrero, 2USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., Kerrville, TX, 78028, USA.; Kylie G Bendele, 2USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., Kerrville, TX, 78028, USA.; Glen A Scoles, 4USDA-ARS Animal Disease Research Unit, Washington State University, 3003 ADBF, Pullman, WA, 99164, USA. |
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Título: |
Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
BMC Microbiology, v.11, p. 1-11, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. Raw data from bTEFAP were screened and trimmed based upon quality scores and binned into individual sample collections. Bacteria identified to the species level include Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, Staphylococcus sciuri, Serratia marcescens, Corynebacterium glutamicum, and Finegoldia magna. One hundred twenty-one bacterial genera were detected in all the life stages and tissues sampled. The total number of genera identified by tick sample comprised: 53 in adult males, 61 in adult females, 11 in gut tissue, 7 in ovarian tissue, and 54 in the eggs. Notable genera detected in the cattle tick include Wolbachia, Coxiella, and Borrelia. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota associated with R. microplus. This report represents the first survey of the bacteriome in the cattle tick using non-culture based molecular approaches. Comparisons of our results with previous bacterial surveys provide an indication of geographic variation in the assemblages of bacteria associated with R. microplus. Additional reports on the identification of new bacterial species maintained in nature by R. microplus that may be pathogenic to its vertebrate hosts are expected as our understanding of its microbiota expands. Increased awareness of the role R. microplus can play in the transmission of pathogenic bacteria will enhance our ability to mitigate its economic impact on animal agriculture globally. This recognition should be included as part of analyses to assess the risk for re-invasion of areas like the United States of America where R. microplus was eradicated. MenosTicks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. Raw data from bTEFAP were screened and trimmed based upon quality scores and binned into individual sample collections. Bacteria identified to the species level include Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, Staphylococcus sciuri, Serratia marcescens, Corynebacterium glutamicum, and Finegoldia magna. One hundred twenty-one bacterial genera were detected in all the life stages and tissues sampled. The total number of genera identified by tick sample comprised: 53 in adult males, 61 in adult females, 11 in gut tissue, 7 in ovarian tissue, and 54 in the eggs. Notable genera detected in the cattle tick include Wolbachia, Coxiella, and Borrelia. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota ... Mostrar Tudo |
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Thesagro: |
Boophilus Microplus; Carrapato; Sanidade Animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39345/1/bmc-microbiology.pdf
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Marc: |
LEADER 03046naa a2200217 a 4500
001 1897418
005 2011-08-05
008 2011 bl uuuu u00u1 u #d
100 1 $aANDREOTTI, R.
245 $aAssessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing.$h[electronic resource]
260 $c2011
520 $aTicks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. Raw data from bTEFAP were screened and trimmed based upon quality scores and binned into individual sample collections. Bacteria identified to the species level include Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, Staphylococcus sciuri, Serratia marcescens, Corynebacterium glutamicum, and Finegoldia magna. One hundred twenty-one bacterial genera were detected in all the life stages and tissues sampled. The total number of genera identified by tick sample comprised: 53 in adult males, 61 in adult females, 11 in gut tissue, 7 in ovarian tissue, and 54 in the eggs. Notable genera detected in the cattle tick include Wolbachia, Coxiella, and Borrelia. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota associated with R. microplus. This report represents the first survey of the bacteriome in the cattle tick using non-culture based molecular approaches. Comparisons of our results with previous bacterial surveys provide an indication of geographic variation in the assemblages of bacteria associated with R. microplus. Additional reports on the identification of new bacterial species maintained in nature by R. microplus that may be pathogenic to its vertebrate hosts are expected as our understanding of its microbiota expands. Increased awareness of the role R. microplus can play in the transmission of pathogenic bacteria will enhance our ability to mitigate its economic impact on animal agriculture globally. This recognition should be included as part of analyses to assess the risk for re-invasion of areas like the United States of America where R. microplus was eradicated.
650 $aBoophilus Microplus
650 $aCarrapato
650 $aSanidade Animal
700 1 $aPÉREZ DE LEÓN, A. A.
700 1 $aDOWD, S. E.
700 1 $aGUERRERO, F. D.
700 1 $aBENDELE, K. G.
700 1 $aSCOLES, G. A.
773 $tBMC Microbiology$gv.11, p. 1-11, 2011.
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Embrapa Gado de Corte (CNPGC) |
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