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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
09/08/1995 |
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Data da última atualização: |
05/04/2017 |
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Autoria: |
NANTULYA, V. M.; MUSOKE, A. J.; RURANGIRWA, F. R.; MOLOO, S. K. |
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Título: |
Resistance of cattle to tsetse-transmitted challenge with Trypanosoma brucei or Trypanosoma congolense after spontaneous recovery from syringe passaged infections. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
Infection and Immunity, v.43, n.2, p.735-738, 1984. |
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Idioma: |
Inglês |
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Conteúdo: |
Groups of cattle were inoculated intravenously with cloned populations of bloodstream forms of Trypanosoma brucei or Trypanosoma congolense. All five steers infected with T. brucei IL Tat 2.1 and six of the eight sters infected with T. congolense IL 13-E14 became aparsitemic within 16 and 32 weeks postinfection, respectively. Examination of sera from animals infected with T. brucei by indirect immunofluorescence and neutralization assays revealed the presence of antibodies against all the metacyclic variable antigen types (VATs) of the infecting clone. The neutralizing capacity of the sera increased with the course of infection from 1:10 at 2 monts to 1:100 at 3 to 4 months postinfection. The recovered animals were completely immune to challenge by Glossina morsitans subsp. centralis infected with clone IL Tat 2.1 which had initiated the infection, as well as wich another clone (IL Tat 2.2) belonging to the same serodeme but they were susceptible to a tsetse-transmited heterologous challenge with isolate STIB 367-H. Similar results were obtained with sera from T. congolense IL 13-E14-infected steers. The six steers inbfected with a different T. congolense IL Nat 3.1 clone did not recover spotaneously; however, 2 months postinfection, sera from five of them also contained neutralizing antibodies against IL Nat 3.1 metacyclic VATs. These results indicate that some of the bloodstream VATs that arise during the course of a chronic infection possess surface epitopes in their variable surface glycoproteins that are identical to those of the metacyclic VATs. It is suggested that in chronic infection, the infecting trypanosomes could exhaust their VAT repertoire, including those that cross-react with metacyclies, threby leading to both self-cure and subsequent immunity to homologous cyclically transmited challenge. MenosGroups of cattle were inoculated intravenously with cloned populations of bloodstream forms of Trypanosoma brucei or Trypanosoma congolense. All five steers infected with T. brucei IL Tat 2.1 and six of the eight sters infected with T. congolense IL 13-E14 became aparsitemic within 16 and 32 weeks postinfection, respectively. Examination of sera from animals infected with T. brucei by indirect immunofluorescence and neutralization assays revealed the presence of antibodies against all the metacyclic variable antigen types (VATs) of the infecting clone. The neutralizing capacity of the sera increased with the course of infection from 1:10 at 2 monts to 1:100 at 3 to 4 months postinfection. The recovered animals were completely immune to challenge by Glossina morsitans subsp. centralis infected with clone IL Tat 2.1 which had initiated the infection, as well as wich another clone (IL Tat 2.2) belonging to the same serodeme but they were susceptible to a tsetse-transmited heterologous challenge with isolate STIB 367-H. Similar results were obtained with sera from T. congolense IL 13-E14-infected steers. The six steers inbfected with a different T. congolense IL Nat 3.1 clone did not recover spotaneously; however, 2 months postinfection, sera from five of them also contained neutralizing antibodies against IL Nat 3.1 metacyclic VATs. These results indicate that some of the bloodstream VATs that arise during the course of a chronic infection possess surface epitopes in their vari... Mostrar Tudo |
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Thesaurus Nal: |
Trypanosoma brucei; Trypanosoma congolense. |
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Categoria do assunto: |
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Marc: |
LEADER 02445naa a2200181 a 4500
001 1785403
005 2017-04-05
008 1984 bl --- 0-- u #d
100 1 $aNANTULYA, V. M.
245 $aResistance of cattle to tsetse-transmitted challenge with Trypanosoma brucei or Trypanosoma congolense after spontaneous recovery from syringe passaged infections.
260 $c1984
520 $aGroups of cattle were inoculated intravenously with cloned populations of bloodstream forms of Trypanosoma brucei or Trypanosoma congolense. All five steers infected with T. brucei IL Tat 2.1 and six of the eight sters infected with T. congolense IL 13-E14 became aparsitemic within 16 and 32 weeks postinfection, respectively. Examination of sera from animals infected with T. brucei by indirect immunofluorescence and neutralization assays revealed the presence of antibodies against all the metacyclic variable antigen types (VATs) of the infecting clone. The neutralizing capacity of the sera increased with the course of infection from 1:10 at 2 monts to 1:100 at 3 to 4 months postinfection. The recovered animals were completely immune to challenge by Glossina morsitans subsp. centralis infected with clone IL Tat 2.1 which had initiated the infection, as well as wich another clone (IL Tat 2.2) belonging to the same serodeme but they were susceptible to a tsetse-transmited heterologous challenge with isolate STIB 367-H. Similar results were obtained with sera from T. congolense IL 13-E14-infected steers. The six steers inbfected with a different T. congolense IL Nat 3.1 clone did not recover spotaneously; however, 2 months postinfection, sera from five of them also contained neutralizing antibodies against IL Nat 3.1 metacyclic VATs. These results indicate that some of the bloodstream VATs that arise during the course of a chronic infection possess surface epitopes in their variable surface glycoproteins that are identical to those of the metacyclic VATs. It is suggested that in chronic infection, the infecting trypanosomes could exhaust their VAT repertoire, including those that cross-react with metacyclies, threby leading to both self-cure and subsequent immunity to homologous cyclically transmited challenge.
650 $aTrypanosoma brucei
650 $aTrypanosoma congolense
700 1 $aMUSOKE, A. J.
700 1 $aRURANGIRWA, F. R.
700 1 $aMOLOO, S. K.
773 $tInfection and Immunity$gv.43, n.2, p.735-738, 1984.
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Registro original: |
Embrapa Pantanal (CPAP) |
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