Data/hora: 29/05/2024 15:17:36

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 24/01/2006
Data da última atualização: | 29/05/2018
Autoria: | WALLACE, J. C.; LOPES, M. A.; PAIVA, E.; LARKINS, B. A.
Afiliação: | EMBRAPA-CNPMS.
Título: | New methods for extraction and quantitation of zeins reveal a high content of gamma -zein in modified opaque-2 maize.
Ano de publicação: | 1990
Fonte/Imprenta: | Plant Physiology, Bethesda, v. 92, n. 1, p. 191-196, 1990.
Idioma: | Inglês
Conteúdo: | We have developed methods for quantitative extraction and analysis of zeins from maize (Zea mays L.) flour. Extraction involved solubilization of total endosperm proteins in an alkaline buffer containing SOS and 2-mercaptoethanol with subsequent precipitation of nonzein proteins by the addition of ethanol to 70%. Analysis of these proteins by SOS-PAGE with Coomassie bluestaining and by Western bloHing and ELlSA assay with zein antibodies revealed that this extraction method is more quantitative than the traditional Landry-Moureaux procedure, especially for the (J-and')'-zeins. This method was used to extract and analyze the zein content of several 'Quality Protein Maize' (QPM) varieties developed by the International Maize and Wheat Improvement Center. QPM varieties contain 'modifiergenes' that confer a vitreous phenotype on opaque·2 genotypes, while maintaining the elevated levels of Iysine and tryptophan characteristic of this mutant. This analysis revealed that the QPM types contain 2 to 4 times the amount of the ')'-zein than unmodified opaque-2 or normal maize varieties. Possible relationships between the high expression of the ')'-zein and the modified opaque phenotype are discussed.
Thesagro: | Melhoramento genético vegetal; Milho.
Thesaurus Nal: | Corn; Plant breeding.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (18288-1) - UPC - AP - DD -  -  -  - DIS