Data/hora: 23/06/2024 10:21:49

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 21/11/2017
Data da última atualização: | 16/05/2024
Autoria: | MARTINI, C. L.; LANGE, C. C.; BRITO, M. A. V. P. e; RIBEIRO, J. B.; MENDONCA, L. C.; VAZ, E. K.
Afiliação: | CAROLINE LOPES MARTINI, UFRJ; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; ELIANA KNACKFUSS VAZ, UNIVERSIDADE DO ESTADO DE SANTA CATARINA.
Título: | Characterization of penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine milk samples in Minas Gerais, Brazil.
Ano de publicação: | 2017
Fonte/Imprenta: | Journal of Dairy Research, v. 84, p. 202-205, 2017.
Idioma: | Inglês
Conteúdo: | This Regional Research Communication describes the characterisation of ampicillin, penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine subclinical mastitis in Minas Gerais State, Brazil. Ninety S. aureus isolates from bovine mastitis exhibiting phenotypic resistance to ampicillin, penicillin and/or tetracycline were selected for this study. The minimum inhibitory concentration (MIC) of each antibiotic was determined using the E-Test® and the production of beta-lactamase was determined by cefinase disks. The resistance genes blaZ, tet(K), tet(L), tet(M), and tet(O) were investigated by PCR in all of the isolates. The MIC results classified 77, 83 and 71% of the isolates as resistant to ampicillin, penicillin and tetracycline, respectively. The MIC50 and MIC90 were, respectively, 1 and 2 µg/ml for ampicillin, 0·5 and 1 µg/ml for penicillin and 32 and 64 µg/ml for tetracycline. Eighty-six per cent of beta-lactamase producing isolates were detected. Of the 90 isolates investigated, 97% amplified blaZ, 84% amplified tet(K), 9% amplified tet(L), 2% amplified tet(M) and 1% amplified tet(O). Seventy-nine isolates (88%) showed blaZ together with at least one tet gene. S. aureus isolates showed high MIC50 and MIC90 values for the three antimicrobials. The blaZ and tet(K) genes were widespread in the herds studied, and most of the isolates harboured blaZ and tet(K) concomitantly.
Palavras-Chave: | BlaZ; E-Test®; MIC; Tet genes.
Thesaurus Nal: | beta-lactamase.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (23712-1) - UPC - AP - DD -  -  -  - DIS