Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
17/09/2007 |
Data da última atualização: |
08/03/2010 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
EGITO, A. A. do; FUCK, B. H.; MCMANUS, C.; PAIVA, S. R.; ALBUQUERQUE, M. do S. M.; SANTOS, S. A.; ABREU, U. G. P. de; SILVA, J. A. da; SERENO, F. T. P. de S.; MARIANTE, A. da S. |
Afiliação: |
Andréa Alves do Egito, Embrapa Recursos Genéticos e Biotecnologia; Beatriz Helena Fuck, UnB; Concepta McManus, UnB; Samuel Resende Paiva, UnB; Maria do Socorro Maués Albuquerque, UnB; Sandra Aparecida Santos, Embrapa Pantanal; Urbano Gomes Pinto de Abreu, Embrapa Pantanal; Joaquim Augusto da Silva, Associação Brasileira dos Criadores de Cavalo Pantaneiro; Fabiana Tavares Pires de Souza Sereno, Bolsista do CNPq; Artur da Silva Mariante, Embrapa Recursos Genéticos e Biotecnologia. |
Título: |
Genetic variability of Pantaneiro horse using RAPD-PCR markers. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Separata de: Revista Brasileira de Zootecnia, v.36, n. 4, p.799-806, 2007. |
Idioma: |
Inglês |
Conteúdo: |
Blood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic ands were generated. The analysis of molecular variance (A M O V A) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75,47%). analysis of the genetic variability among pairs of population presented higher estimates for the five Pantaneiro populations with the Arabian breed, while lowest estimates were presented by pairs formed among the Pantaneiro populations with the Mangalarga Marchador . Highest genic diversity was shown by the Pantaneiro (0.3396), which also showed highest distance with the Arabian and lowest with Mangalarga Marchador breed. UPGMA dendrogram showed distinet differences between naturalized (Pantaneiro and Mangalarga Marchador) and exotic (Arabian and Thoroughbred) breeds. In the dendrogram generated by UPMGA method, the similarity matrix generated by the Jaccard coefficient showed distinction between the naturalised breeds, Pantaneiro and Mangalarga Marchador, and the exotic breeds, Árab and English Thoroughbred. Results suggest that the Pantaneiro presents a higher genetic variability than the other studied breeds and has a close relationship with the Mangalarga Marchador. MenosBlood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic ands were generated. The analysis of molecular variance (A M O V A) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75,47%). analysis of the genetic variability among pairs of population presented higher estimates for the five Pantaneiro populations with the Arabian breed, while lowest estimates were presented by pairs formed among the Pantaneiro populations with the Mangalarga Marchador . Highest genic diversity was shown by the Pantaneiro (0.3396), which also showed highest distance with the Arabian and lowest with Mangalarga Marchador breed. UPGMA dendrogram showed distinet differences between naturalized (Pantaneiro and Mangalarga Marchador) and exotic (Arabian and Thoroughbred) breeds. In the dendrogram generated by UPMGA method, the similarity matrix generated by the Jaccard coefficient showed distinction between the naturalised breeds, Pantaneiro and Mangalarga Marchador, and the exotic breeds, Árab and English Thoroughbred. Results suggest that the Pantaneiro presents ... Mostrar Tudo |
Palavras-Chave: |
AMOVA; Arabian; English Thoroughbred; genetic diversity; genetic resource; Mangalarga Marchador. |
Categoria do assunto: |
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Marc: |
LEADER 02465naa a2200301 a 4500 001 1812437 005 2010-03-08 008 2007 bl --- 0-- u #d 100 1 $aEGITO, A. A. do 245 $aGenetic variability of Pantaneiro horse using RAPD-PCR markers. 260 $c2007 520 $aBlood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic ands were generated. The analysis of molecular variance (A M O V A) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75,47%). analysis of the genetic variability among pairs of population presented higher estimates for the five Pantaneiro populations with the Arabian breed, while lowest estimates were presented by pairs formed among the Pantaneiro populations with the Mangalarga Marchador . Highest genic diversity was shown by the Pantaneiro (0.3396), which also showed highest distance with the Arabian and lowest with Mangalarga Marchador breed. UPGMA dendrogram showed distinet differences between naturalized (Pantaneiro and Mangalarga Marchador) and exotic (Arabian and Thoroughbred) breeds. In the dendrogram generated by UPMGA method, the similarity matrix generated by the Jaccard coefficient showed distinction between the naturalised breeds, Pantaneiro and Mangalarga Marchador, and the exotic breeds, Árab and English Thoroughbred. Results suggest that the Pantaneiro presents a higher genetic variability than the other studied breeds and has a close relationship with the Mangalarga Marchador. 653 $aAMOVA 653 $aArabian 653 $aEnglish Thoroughbred 653 $agenetic diversity 653 $agenetic resource 653 $aMangalarga Marchador 700 1 $aFUCK, B. H. 700 1 $aMCMANUS, C. 700 1 $aPAIVA, S. R. 700 1 $aALBUQUERQUE, M. do S. M. 700 1 $aSANTOS, S. A. 700 1 $aABREU, U. G. P. de 700 1 $aSILVA, J. A. da 700 1 $aSERENO, F. T. P. de S. 700 1 $aMARIANTE, A. da S. 773 $tSeparata de: Revista Brasileira de Zootecnia$gv.36, n. 4, p.799-806, 2007.
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Registro original: |
Embrapa Pantanal (CPAP) |
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