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Registros recuperados : 71 | |
21. | | PELLEGRIN, A. O.; FIGUEIREDO, J. F.; LEITE, R. C.; MIRANDA, K. L.; RAVAGLIA, E.; LAGE, A. P. Diagnostico para Campilobacteriose genital bovina pelo ELISA IgA. In: CONGRESSO BRASILEIRO DE BUIATRIA, 4., 2001, Campo Grande. Resumos. Campo Grande: [s.n.], 2001. p.192. Biblioteca(s): Embrapa Pantanal. |
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22. | | FIGUEIREDO, J. F.; PELLEGRIN, A. O.; FOSCOLO, C. B.; MACHADO, R. P.; MIRANDA, K. L.; LAGE, A. P. Evaluation of direct fluorescent antibody test for the diagnosis of Bovine Genital Campylobacteriosis. Revista Latinoamericana de Microbiologia, v.44, n.3/4, p.118-123, 2002. Biblioteca(s): Embrapa Pantanal. |
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23. | | REINATO, A. P. R.; FOSCOLO, C. B.; PELLEGRIN, A. O.; CANELLA FILHO, C.; FIGUEIREDO, J. F.; LAGE, A. P. Frequencia de campilobacteriose genital bovina em rebanhos leiteiros do Estado de Minas Gerais. In: ENCONTRO DE PESQUISA DA ESCOLA DE VETERINARIA, 16., 1998, Belo Horizonte. Anais... Belo Horizonte: UFMG-Escola de Veterinaria, 1998. p.87 Biblioteca(s): Embrapa Pantanal. |
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24. | | FOSCOLO, C. B.; PELLEGRIN, A. O.; PINHEIRO, L. M. S.; LOBATO, Z. I. P.; LEITE, R. C.; LAGE, A. P. Frequencia da infeccao pelo virus da lingua azul em bovinos do Pantanal Matogrossense. In: CONGRESSO BRASILEIRO DE VETERIONARIA, 27.; CONGRESSO PAULISTA DE VETERINARIA, 5.; CONFERENCIA ANUAL DA SOCIEDADE PAULISTA DE MEDICINA VETERINARIA, 55.; EXPOVET, 6., 2000, Aguas de Lindoia. Resumos. Aguas de Lindoia: [s.n.], 2000. p.92. Biblioteca(s): Embrapa Pantanal. |
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25. | | PELLEGRIN, A. O.; BARBOSA, E. F.; REINATO, A. P. R.; LEITE, R. C.; RAVAGLIA, E.; LAGE, A. P. Padronizacao de um ensaio imunoenzimatico para deteccao de IgA anti-Campylobacter fetus em muco cervico-vaginal de femeas bovinas. In: ENCONTRO DE PESQUISA DA ESCOLA DE VETERINARIA, 16., 1998, Belo Horizonte. Anais... Belo Horizonte: UFMG-Escola de Veterinaria, 1998. p.85 Biblioteca(s): Embrapa Pantanal. |
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29. | | MUSSI, J. M. S.; GOUVEIA, A. M. G.; CORTEZ, A.; LAGE, A. P.; GUIMARAES, A. S.; HEINEMANN, M. B. Use of serological diagnostic techniques in the control and eradication of caprine arthritis encephalitis: an update. Brazilian Journal of Veterinary Research and Animal Science, v. 52, n. 4, p. 283-297, 2015. Biblioteca(s): Embrapa Gado de Leite. |
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30. | | GONÇALVES, M. S.; OLIVEIRA, M. M. de; ANDRADE, R. S.; OLIVEIRA, L. F. de; GUIMARÃES, A. S.; GODFROID, J.; LAGE, A. P.; DORNELES, E. M. S. Systematic review on the effectiveness of Brucella abortus S19 and RB51 vaccine strains: effectiveness of brucella abortus vaccines. In: ENCONTRO NACIONAL DA REDE EM PESQUISA E INOVAÇÃO EM SANIDADE E PECUÁRIA LEITEIRA, 1., 2023, Lavras. Anais do evento. Lavras: Universidade Federal de Lavras, 2023. Biblioteca(s): Embrapa Gado de Leite. |
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31. | | GUIMARAES, A. S.; CARMO, F. B.; HEINEMANN, M. B.; PORTELA, R. W. D.; MEYER, R.; LAGE, A. P.; SEYFFERT, N.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G. High sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of Minas Gerais, Brazil. BMC Veterinary Research, v. 7, article 68, 2011. Biblioteca(s): Embrapa Gado de Leite. |
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32. | | PELLEGRIN, A. O.; XAVIER, I. G.; LAGE, A. P.; ALVES, T. M.; STYNEN, A. P. R.; TOMICH, R. G. P.; RAVAGLIA, E. A campilobacteriose como um dos fatores de baixo indice de fertilidade em bovinos de corte no Pantanal mato-grossense: uma investigacao preliminar. In: CONGRESSO DE INICIACAO CIENTIFICA EM CIENCIAS AGRARIAS, BIOLOGICAS E AMBIENTAIS, 1., 2003, Sao Paulo. Resumos... São Paulo: Instituto Biológico, 2003. Arquivo do Instituto Biológico, São Paulo, v.70, supl. 2, 2003. 5AC. CD-ROM. Biblioteca(s): Embrapa Pantanal. |
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33. | | LAGE, A. P.; PELLEGRIN, A. O.; COSTA, G. M.; SILVA, N.; REINATO, A. P. R.; GOMES, L. I.; GUIMARAES, P. H. S.; LEITE, R. C. Campilobacteriose genital bovina: diagnostico na Escola de Veterinaria da UFMG de 1976 a 1996. Revista Brasileira de Reproducao Animal, v.21, n.2, p.164-166, 1997. Anais do 12 Congresso Brasileirto de Reproducao Animal. Biblioteca(s): Embrapa Pantanal. |
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34. | | PELLEGRIN, A. O.; FOSCOLO, C. B.; SERENO, J. R. B.; ABREU, U. G. P. de; RAVAGLIA, E.; LEITE, R. C.; LAGE, A. P. Campilobacteriose genital bovina no Nucleo de Conservacao de Bovino Pantaneiro "in situ" da Embrapa Pantanal. In: SIMPOSIO IBERO-AMERICANO SOBRE CONSERVACAO DE RECURSOS GENETICOS ANIMAIS, 1., 2000, Corumba, MS. Anais... Corumba: Embrapa Pantanal, 2000. p.12. Biblioteca(s): Embrapa Pantanal. |
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35. | | STYNEN, A. P. R.; PELLEGRIN, A. O.; FÓSCOLO, C. B.; FIGUEIREDO, J. F.; CANELLA FILHO, C.; LEITE, R. C.; LAGE, A. P. Campilobacteriose genital bovina em rebanhos leiteiros com problemas reprodutivos da microrregião de Varginha - Minas Gerais. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v.55, n.6, p.766-769, dez., 2003. Biblioteca(s): Embrapa Pantanal. |
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36. | | MORAIS-RIOS, P. A. G.; ALVES, T. M.; DORNELES, E. M. S.; STYNEN, A. P. R.; COTTORELLO, A. C. P.; LAURA-FILGUEIRAS, A. L.; LAGE, A. P. Adherence to and invasion of HeLa cells by Campylobacter spp. strains isolated from animals. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 7, p. 1293-1299, julho 2018 Título em português: Adesão e invasão de células HeLa por amostras de Campylobacter spp. isoladas de animais. Biblioteca(s): Embrapa Unidades Centrais. |
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37. | | PELLEGRIN, A. O.; LEITE, R. M. H.; LEITE, R. C.; SERENO, J. R. B.; RAVAGLIA, E.; ABREU, U. G. P. de; LAGE, A. P. Acompanhamento da brucelose no Nucleo de Conservacao "in situ" do bovino pantaneiro da Fazenda Nhumirim em 1999. In: SIMPOSIO IBERO-AMERICANO SOBRE CONSERVACAO DE RECURSOS GENETICOS ANIMAIS, 1., 2000, Corumba, MS. Anais... Corumba: Embrapa Pantanal, 2000. p.7. Biblioteca(s): Embrapa Pantanal. |
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38. | | PELLEGRIN, A. O.; SERENO, J. R. B.; LEITE, R. C.; RAVAGLIA, E.; ABREU, U. G. P. de; LAGE, A. P.; LOBATO, Z. I. P. Anticorpos para os virus da diarreia bovina a virus em animais do Nucleo de Conservacao de Bovino Pantaneiro. In: SIMPOSIO IBERO-AMERICANO SOBRE CONSERVACAO DE RECURSOS GENETICOS ANIMAIS, 1., 2000, Corumba, MS. Anais... Corumba: Embrapa Pantanal, 2000. p.10. Biblioteca(s): Embrapa Pantanal. |
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39. | | LEITE, R. C.; PELLEGRIN, A. O.; MARTINS, N. E.; SILVA, N.; GOMES, L. I.; COSTA, G. M.; REINATO, A. P. R.; GUIMARAES, P. H. S.; LAGE, A. P. Tricomonose bovina: diagnostico realizados na Escola de Veterinaria da UFMG no periodo de 1979 a 1995. Revista Brasileira de Reproducao Animal, v.21, n.2, p.166-168, 1997. Anais do 12 Congresso Brasileiro de Reproducao Animal. Biblioteca(s): Embrapa Pantanal. |
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40. | | PELLEGRIN, A. O.; MARTINS, N. E.; LAGE, A. P.; COSTA, G. M. da; GOMES, L. I.; REINATO, A. P. R.; LEITE, R. C. Tricomonose bovina no Estado de Minas Gerais: ocorrencia e tratamento. Revista Brasileira de Medicina Veterinaria, Rio de Janeiro, v.20, n.6, p.244-248, nov./dez., 1998. Biblioteca(s): Embrapa Pantanal. |
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Registros recuperados : 71 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
16/12/2011 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
Idioma: |
Inglês |
Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
Palavras-Chave: |
ERIC-PCR; RAPD. |
Thesagro: |
Corynebacterium Pseudotuberculosis. |
Thesaurus NAL: |
caseous lymphadenitis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02507naa a2200265 a 4500 001 1910089 005 2024-02-05 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI 100 1 $aGUIMARAES, A. S. 245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource] 260 $c2011 520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. 650 $acaseous lymphadenitis 650 $aCorynebacterium Pseudotuberculosis 653 $aERIC-PCR 653 $aRAPD 700 1 $aDORNELES, E. M. S. 700 1 $aANDRADE, G. I. 700 1 $aLAGE, A. P. 700 1 $aMIYOSHI, A. 700 1 $aAZEVEDO, V. 700 1 $aGOUVEIA, A. M. G. 700 1 $aHEINEMANN, M. B. 773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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