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| Registros recuperados : 71 | |
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| 42. | | DORNELES, E. M. S.; MORCATTI, F. C.; GUIMARAES, A. S.; LOBATO, Z. I. P.; LAGE, A. P.; GONÇALVES, V. S. P.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra o virus da lingua azul em ovinos do Distrito Federal. Semina, v. 33, n. 4, p. 1521-1524, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 43. | | PELLEGRIN, A. O.; COSTA, G. M.; SERENO, J. R. B.; RAVAGLIA, E.; GOMES, L. I.; REINATO, A. P. R.; LAGE, A. P.; LEITE, R. C. Prevalencia de Campylobacter fetus em touros do Pantanal Mato-Grossense. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINARIA, 25., 1997, Gramado. Resumos. Porto Alegre: [s.n.] 1997. p.176.| Biblioteca(s): Embrapa Pantanal. |
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| 44. | | PELLEGRIN, A. O.; GUIMARAES, P. H. S.; FIGUEIREDO, J.; ARAUJO, V. E. M.; MOREIRA, E. C.; RAVAGLIA, E.; PAULA, A. B.; LEITE, R. C.; LAGE, A. P. Prevalencia de Leptospira interrogans do sorogrupo hardjo em bovinos de corte no Pantanal Mato-Grossense. In: ENCONTRO DE PESQUISA DA ESCOLA DE VETERINARIA, 16., 1998, Belo Horizonte. Anais... Belo Horizonte: UFMG-Escola de Veterinaria, 1998. p.86| Biblioteca(s): Embrapa Pantanal. |
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| 46. | | GUIMARAES, A. S.; CARMO, F. B.; HEINEMANN, M. B.; PORTELA, R. W. D.; MEYER, R.; LAGE, A. P.; SEYFFERT, N.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G. High sero-prevalence of caseous lymphadenitis identified in slaughterhouse samples as a consequence of deficiencies in sheep farm management in the state of Minas Gerais, Brazil. BMC Veterinary Research, v. 7, article 68, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 47. | | PELLEGRIN, A. O.; SERENO, J. R. B.; LEITE, R. C.; RAVAGLIA, E.; ABREU, U. G. P. de; LAGE, A. P.; ROCHA, M. A.; LOBATO, Z. I. P. Frequencia de anticorpos para o virus da rinotraqueite infecciosa bovina em animais do Nucleo de Conservacao de Bovino Pantaneiro da Fazenda Nhumirim - Embrapa Pantanal. In: SIMPOSIO IBERO-AMERICANO SOBRE CONSERVACAO DE RECURSOS GENETICOS ANIMAIS, 1., 2000, Corumba, MS. Anais... Corumba: Embrapa Pantanal, 2000. p.33.| Biblioteca(s): Embrapa Pantanal. |
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| 49. | | GONÇALVES, M. S.; OLIVEIRA, M. M. de; ANDRADE, R. S.; OLIVEIRA, L. F. de; GUIMARÃES, A. S.; GODFROID, J.; LAGE, A. P.; DORNELES, E. M. S. Systematic review on the effectiveness of Brucella abortus S19 and RB51 vaccine strains: effectiveness of brucella abortus vaccines. In: ENCONTRO NACIONAL DA REDE EM PESQUISA E INOVAÇÃO EM SANIDADE E PECUÁRIA LEITEIRA, 1., 2023, Lavras. Anais do evento. Lavras: Universidade Federal de Lavras, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 50. | | LEITE, R. C.; PELLEGRIN, A. O.; MARTINS, N. E.; SILVA, N.; GOMES, L. I.; COSTA, G. M.; REINATO, A. P. R.; GUIMARAES, P. H. S.; LAGE, A. P. Tricomonose bovina: diagnostico realizados na Escola de Veterinaria da UFMG no periodo de 1979 a 1995. Revista Brasileira de Reproducao Animal, v.21, n.2, p.166-168, 1997. Anais do 12 Congresso Brasileiro de Reproducao Animal.| Biblioteca(s): Embrapa Pantanal. |
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| 51. | | PELLEGRIN, A. O.; MARTINS, N. E.; LAGE, A. P.; COSTA, G. M. da; GOMES, L. I.; REINATO, A. P. R.; LEITE, R. C. Tricomonose bovina no Estado de Minas Gerais: ocorrencia e tratamento. Revista Brasileira de Medicina Veterinaria, Rio de Janeiro, v.20, n.6, p.244-248, nov./dez., 1998.| Biblioteca(s): Embrapa Pantanal. |
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| 52. | | FOSCOLO, C. B.; PELLEGRIN, A. O.; PINHEIRO, L. M. S.; SERENO, J. R. B.; ABREU, U. G. P. de; RAVAGLIA, E.; LEITE, R. C.; LAGE, A. P.; LOBATO, Z. I. P. Anticorpos contra o virus da lingua azul no Nucleo de Conservacao do Bovino Pantaneiro. In: SIMPOSIO IBERO-AMERICANO SOBRE CONSERVACAO DE RECURSOS GENETICOS ANIMAIS, 1., 2000, Corumba, MS. Anais... Corumba: Embrapa Pantanal, 2000. p.9.| Biblioteca(s): Embrapa Pantanal. |
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| 53. | | PELLEGRIN, A. O.; LEITE, R. M. H.; SERENO, J. R. B.; RAVAGLIA, E.; HERMANN, G. P.; ABREU, U. G. P. de; MOREIRA, E. C.; LEITE, R. C.; LAGE, A. P. Leptospirose no Nucleo de Conservacao do Bovino Pantaneiro da Fazenda Nhumirim, sub-regiao da Nhecolandia, Pantanal Mato-Grossense em 1999. In: SIMPOSIO IBERO-AMERICANO SOBRE CONSERVACAO DE RECURSOS GENETICOS ANIMAIS, 1., 2000, Corumba, MS. Anais... Corumba: Embrapa Pantanal, 2000. p.36.| Biblioteca(s): Embrapa Pantanal. |
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| 54. | | DORNELES, E. M. S.; ANDRADE, G. I.; SANTOS, E. L. DE S.; SANTANA, J. A.; LAGE, A. P.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. DE S.; MIYOSHI, A.; AZEVEDO, V. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR. In: Encontro Nacional de Patologia Veterinária, 15., 2011, Goiânia. Resumos...| Biblioteca(s): Embrapa Gado de Leite. |
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| 55. | | DORNELES, E. M. S.; ANDRADE, G. I.; SANTOS, E. L. DE S.; SANTANA, J. A.; LAGE, A. P.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. DE S.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using eric-PCR. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 56. | | DORNELES, E. M. S.; SANTANA, J. A.; ANDRADE, G. I.; SANTOS, E. L. S.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. S.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; LAGE, A. P.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR. Genetics and Molecular Research, v. 153, n. 3/4, p. 299-306, 2011| Biblioteca(s): Embrapa Gado de Leite. |
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| 57. | | DORNELES, E. M. S.; MORCATTI, F. C.; GUIMARÃES, A. de S.; LOBATO, Z. I. P.; LAGE, A. P.; GONÇALVES, V. S. P.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalence of bluetongue virus antibodies in sheep from Distrito Federal, Brazil. Semina: Ciências Agrárias, Londrina, v. 33, n. 4, p. 1521-1524, jul./ago., 2012.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 58. | | CARMO, F. B. DO; GUIMARAES, A. S.; PAULETTI, R. B.; LAGE, A. P.; GONÇALVES, V. S. P.; MEYER, R.; PORTELA, R. W. B.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra a linfadenite caseosa em criações comerciais de ovinos no Distrito Federal, Brasil. Arquivos do Instituto Biológico, v. 79, n. 2, p. 293-298, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 59. | | CARMO, F. B. do; GUIMARÃES, A. S.; PAULETTI, R. B.; LAGE, A. P.; GONÇALVES, V. S. P.; MEYER, R.; PORTELA, R. W. D.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra a linfadenite caseosa em criações comerciais de ovinos no Distrito Federal, Brasil. Arquivos do Instituto Biológico, v. 79, n. 2, p. 293-298, abr./jun. 2012.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 60. | | SALES, M. L.; FONSECA JUNIOR, A. A.; SALES, E. B.; COTTORELLO, A. C. P.; ISSA, M. A.; HODON, M. A.; SOARES FILHO, P. M.; RAMALHO, A. K.; SILVA, M. R.; LAGE, A. P.; HEINEMANN, M. B. Evaluation of molecular markers for the diagnosis of Mycobacterium bovis Folia Microbiologica, v. 59, n. 5, p. 433-438, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 71 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/03/2021 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
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Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
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Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
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Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus NAL: |
Vaccines. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02811naa a2200349 a 4500
001 2130440
005 2024-02-06
008 2020 bl uuuu u00u1 u #d
100 1 $aCUNHA, A. F.
245 $aComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.$h[electronic resource]
260 $c2020
520 $aABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.
650 $aVaccines
650 $aStaphylococcus Aureus
653 $aImmunoproteomics
653 $aIntramammary infection
653 $aSerological proteome analysis
700 1 $aANDRADE, H. M.
700 1 $aSOUZA, F. N.
700 1 $aFIALHO JUNIOR, L. C.
700 1 $aROSA, D. L. S. O.
700 1 $aSANCHEZ, E. M. R.
700 1 $aGIDLUND, M.
700 1 $aGOTO, H.
700 1 $aBRITO, M. A. V. P.
700 1 $aGUIMARÃES, A. S.
700 1 $aLAGE, A. P.
700 1 $aREIS, L. C.
700 1 $aLIBERA, A. M. M. P. D.
700 1 $aHEINEMANN, M. B.
700 1 $aCERQUEIRA, M. M. O. P
773 $tJournal of Dairy Science$gv. 103, n. 5, p. 4588-4605, 2020.
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