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| Registros recuperados : 71 | |
| 9. |  | FIGUEIREDO, J. F.; PELLEGRIN, A. O.; LEITE, K. M.; LAGE, A. P. Comparacao entre diferentes preparacoes de antigenos de Campylobacter fetus subso. venerelais para utilizacao em um teste de ELISA IgA. In: CONGRESSO BRASILEIRO DE VETERIONARIA, 27.; CONGRESSO PAULISTA DE VETERINARIA, 5.; CONFERENCIA ANUAL DA SOCIEDADE PAULISTA DE MEDICINA VETERINARIA, 55.; EXPOVET, 6., 2000, Aguas de Lindoia. Resumos. Aguas de Lindoia: [s.n.], 2000. p.88.| Biblioteca(s): Embrapa Pantanal. |
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| 10. | | MIRANDA, K. L.; LAGE, A. P.; MACHADO, R. P.; FIGUEIREDO, J. F.; PELLEGRIN, A. O. Comparacao entre diferentes preparacoes de antigenos de Campylobacter fetus venerealis para utilizacao em um teste de ELISA IgA. In: SEMANA DO CONHECIMENTO DA UFMG, 1.; SEMANA DE INICIACAO CIENTIFICA, 9., 2000, Belo Horizonte. Resumos. Belo Horizonte: UFMG, 2000. p.30.| Biblioteca(s): Embrapa Pantanal. |
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| 15. | | PELEGRIN, A. O.; FIGUEIREDO, J. F.; BARBOSA, E. F.; LEITE, R. C.; LAGE, A. P. Immunoblotting para deteccao de imunoglobulina A anti-Campylobacter fetus em muco cervico-vaginal de femeas bovinas. In: CONGRESSO BRASILEIRO DE VETERIONARIA, 27.; CONGRESSO PAULISTA DE VETERINARIA, 5.; CONFERENCIA ANUAL DA SOCIEDADE PAULISTA DE MEDICINA VETERINARIA, 55.; EXPOVET, 6., 2000, Aguas de Lindoia. Resumos. Aguas de Lindoia: [s.n.], 2000. p.78.| Biblioteca(s): Embrapa Pantanal. |
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| 17. | | FÓSCOLO, C. B.; PELLEGRIN, A. O.; LEITE, R. C.; STYNEN, A. P. R.; LAGE, A. P. Vaccination of bulls against bovine genital campylobacteriosis: a therapeutic approach. Animal Reproduction, v. 2, n. 2, p.122-127, apr./jun., 2005.| Biblioteca(s): Embrapa Pantanal. |
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| 18. | | PELLEGRIN, A. O.; LEITE, R. C.; LAGE, A. P.; FIGUEIREDO, J.; STANCIOLI, E. F. B.; SERENO, J. R. B. Ensaio imunoenzimatico e "imunoblotting" para deteccao de imunoglobulinas a anti- Campylobacter fetus em muco cervico- vaginal de femeas bovinas. In: SEMANA DA POS-GRADUACAO DA UFMG, 3., 2002, Belo Horizonte. Anais... Belo Horizonte: UFMG, 2002. nao paginado.| Biblioteca(s): Embrapa Pantanal. |
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| 19. | | RODRIGUES, R. O.; HERRMANN, G. P.; HEINEMANN, M. B.; LAGE, A. P.; LOPES, L. B.; MOREIRA, E. C. Comparação entre a imunidade induzida em bovinos vacinados com bacterinas polivalentes comerciais e uma monovalente experimental. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 31, n. 1, p. 10-16, 2011.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/03/2021 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
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Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
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Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
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Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus NAL: |
Vaccines. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02811naa a2200349 a 4500
001 2130440
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100 1 $aCUNHA, A. F.
245 $aComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.$h[electronic resource]
260 $c2020
520 $aABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.
650 $aVaccines
650 $aStaphylococcus Aureus
653 $aImmunoproteomics
653 $aIntramammary infection
653 $aSerological proteome analysis
700 1 $aANDRADE, H. M.
700 1 $aSOUZA, F. N.
700 1 $aFIALHO JUNIOR, L. C.
700 1 $aROSA, D. L. S. O.
700 1 $aSANCHEZ, E. M. R.
700 1 $aGIDLUND, M.
700 1 $aGOTO, H.
700 1 $aBRITO, M. A. V. P.
700 1 $aGUIMARÃES, A. S.
700 1 $aLAGE, A. P.
700 1 $aREIS, L. C.
700 1 $aLIBERA, A. M. M. P. D.
700 1 $aHEINEMANN, M. B.
700 1 $aCERQUEIRA, M. M. O. P
773 $tJournal of Dairy Science$gv. 103, n. 5, p. 4588-4605, 2020.
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