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| 41. |  | BONFIM, K.; LACORTE, C.; ARAGÃO, F. J. L.; OLIVEIRA, S. C.; RECH, E. L. Introdução e expressão do gene Lack do antígeno da Leishmaniose em alface (Lactuca Sativa). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 51.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 42. | | LACORTE, C.; ARAGAO, F.; MANSUR, E.; OLIVEIRA, D. E.; CORDEIRO, A. R.; RECH, E. Evaluation of peanut (Arachis hypogaea L.) tissues for particle bombardment-mediated gene transfer. In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 1., 1993, Brasilia. Programas e resumos. Brasilia: EMBRAPA-CENARGEN, 1993. Poster 029. Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 45. | | LACORTE, C.; RIBEIRO, S. G.; LOHUIS, H.; GOLDBACH, R.; PRINS, M. PVX-mediated expression of the N gene in Nicotiana benthamiana blocks Tomato spotted wilt virus. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 41., 2008, Belo Horizonte. [Resumos...]. Tropical Plant Pathology, v. 33, 2008. Suplemento. Resumo VIR-029. p. S132.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 47. | | LACORTE, C. C.; FERREIRA, A. L.; MURAD, A. M.; CUNHA, N. B. da; PINHEIRO, P. V. Seed-based production of recombinant proteins. In: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6. Cap. 6, p. 185-208.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 49. | | RIBEIRO, S. G.; LACORTE, C.; BEENEN, H.; GOLDBACH, R.; PRINS, M. Transient expression of a single-chain antibody using PVX vector leads to enhanced resistance to Tomato spotted wilt virus. Fitopatologia Brasileira, v. 31, suppl., ago. 2006. Anais do: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 39.; ANNUAL MEETING OF THE BRAZILIAN PHYTOPATHOLOGICAL SOCIETY, 39., 2006, Salvador, BA. p. S291.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 50. | | TEIXEIRA, R. P.; BARROS, L. M. G.; LACORTE, C.; CARNEIRO, M.; ALMEIDA, J. D. de. Subcellular localization of the RoIA protein. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 51. | | LACORTE, C. C.; MURAD, A. M.; MADEIRA, L.; CUNHA, N. B.; RECH FILHO, E. L. Subcellular targeting of recombinant Cyanovirin-N, An-HIV microbiocide, in soybean and Nicotiana bethamiana plants. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios. Programa e resumos. [s.l.]: Sociedade Brasileira de Genética, 2009. p. 252.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 52. | | MANSUR, E.; LACORTE, C.; RABELLO, A. C. G.; CORDEIRO, A. R. In Vitro Regeneration of Arachis Villosulicarpa Hoehne from Cotyledon Segments, Leaves and Cell Suspension. Pesquisa Agropecuaria Brasileira, Brasilia, v.28, n.10, p.1143-1146,out.1993| Biblioteca(s): Embrapa Unidades Centrais. |
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| 55. | | NOGUEIRA, M. P.; FONTENELE, R. S.; LACORTE, C. C.; RIBEIRO, S. da G.; REIS, A. M. dos. Proteômica de folhas de tomate (Solanum lycopersicum) inoculadas com Tomato chlorotic mottle virus (ToCMoV). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 029. p. 65| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 57. | | VILLETH, G. R. C.; LACERDA, A. L.; LACORTE, C.; BRASILEIRO, A. C. M.; BOITEUX, L. S.; RIBEIRO, S. G. Involvement of TMV response related-protein in the defense of tomato to tomato chlorotic mottle virus. Journal of the Brazilian Society for Virology, Novo Hamburgo, v. 19, p. 218, Sept./Oct. 2014. Suppl. 2 Abstracts/Posters - Plant and Invertebrate Virology: PIV. Resumo apresentado no 25 Brazilian Congress of Virology & 9 Mercosur Meeting of Virology, 2014 - Ribeirão Preto.| Biblioteca(s): Embrapa Hortaliças. |
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| 58. | | MURAD, A. M.; CUNHA, N.; LACORTE, C. C.; COELHO, M. C. F.; VIANNA, G. R.; RECH FILHO, E. L. Expression, purification and analysis of the anti-HIV Cyanovirin-N produced in transgenic soybeans seeds. BMC Proceedings, v.8, suppl, P.105, 2014. (Open access) Edição do Congress of the Brazilian Biotechnology Society, Florianópolis, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 59. | | LIMA, B. P.; ALVES-FREITAS, D. M. T.; GODINHO, M. T.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G. High incidence of mixed DNA and RNA virus infections in common bean in Central Brazil. Virus Reviews & Research, Brasília, DF, v. 20, n. 2, p. 132-133, Aug./Dec. 2016. Edição dos Resumos do XXVII Brazilian Congress of Virology, XI Mercosur Meeting of Virology, Pirenópolis, GO, Sept. 2016. PIV151.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 60. | | LIMA, B. P.; ALVES-FREITAS, D. M. T.; GODINHO, M. T.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G. High incidence of mixed DNA and RNA virus infections in common bean in Central Brazil. Virus Reviews & Research, Brasília, DF, v. 20, n. 2, p. 132-133, Aug./Dec. 2016. Ref. PIV151. Edição dos Resumos do XXVII Brazilian Congress of Virology, XI Mercosur Meeting of Virology, Pirenópolis, GO, Sept. 2016.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 120 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
21/01/2019 |
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Data da última atualização: |
12/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ALVES-FREITAS, D. M. T.; PINHEIRO-LIMA, B.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G.; MELO, F. L. |
|
Afiliação: |
DIONE M. T. ALVES-FREITAS; BRUNA PINHEIRO-LIMA; JOSIAS CORREA DE FARIA, CNPAF; CRISTIANO CASTRO LACORTE, Cenargen; SIMONE DA GRACA RIBEIRO, Cenargen; FERNANDO L. MELO, UNB. |
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Título: |
Double-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Viruses, v. 11, n. 1, Jan. 2019. |
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ISSN: |
1999-4915 |
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DOI: |
10.3390/v11010090 |
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Idioma: |
Inglês |
|
Conteúdo: |
Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean. |
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Palavras-Chave: |
Rhabdovirus. |
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Thesagro: |
Feijão; Mosaico Dourado; Organismo Transgênico; Phaseolus Vulgaris; Vírus. |
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Thesaurus NAL: |
Bean golden mosaic virus; Beans; Cytorhabdovirus; DsRNA viruses; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191029/1/CNPAF-2019-viruses.pdf
|
|
Marc: |
LEADER 02429naa a2200337 a 4500
001 2104582
005 2020-02-12
008 2019 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v11010090$2DOI
100 1 $aALVES-FREITAS, D. M. T.
245 $aDouble-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line.$h[electronic resource]
260 $c2019
520 $aUsing double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean.
650 $aBean golden mosaic virus
650 $aBeans
650 $aCytorhabdovirus
650 $aDsRNA viruses
650 $aTransgenic plants
650 $aFeijão
650 $aMosaico Dourado
650 $aOrganismo Transgênico
650 $aPhaseolus Vulgaris
650 $aVírus
653 $aRhabdovirus
700 1 $aPINHEIRO-LIMA, B.
700 1 $aFARIA, J. C.
700 1 $aLACORTE, C.
700 1 $aRIBEIRO, S. G.
700 1 $aMELO, F. L.
773 $tViruses$gv. 11, n. 1, Jan. 2019.
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Embrapa Arroz e Feijão (CNPAF) |
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