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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
22/03/2022 |
Data da última atualização: |
22/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FALCÃO, M. V. D.; LAROUCAU, K.; VORIMORE, F.; DESHAYES, T.; SANTANA, V. L. A.; SILVA, K. P. C.; NASCIMENTO, S. A. DO; CASTRO, R. S. DE; ARAUJO, F. R.; MOTA, R. A. |
Afiliação: |
MARCUS V. D. FALCÃO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil.; KARINE LAROUCAU, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França.; FABIEN VORIMORE, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França.; THOMAS DESHAYES, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França.; VANIA L. A. SANTANA, Ministério da Agricultura, Pecuária e Abastecimento, Recife, Pernambuco, Brasil.; KARLA P. C. SILVA, Universidade Federal de Alagoas, Departamento de Medicina Veterinária, Maceió, Alagoas, Brasil.; SERGIO A. DO NASCIMENTO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil.; ROBERTO S. DE CASTRO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil.; FLABIO RIBEIRO DE ARAUJO, CNPGC; RINALDO A. MOTA, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil. |
Título: |
Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017). |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Infection, Genetics and Evolution, v. 99, 2022. |
Idioma: |
Inglês |
Conteúdo: |
Glanders is an infectious zoonosis caused by Burkholderia (B.) mallei that mainly affects equids. The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction?high-resolution melting (PCRHRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe and the Middle East. Whole genome sequencing of two of these strains (UFAL2 and BM_campo 2.1), belonging to different branches, confirmed the HRM typing results and refined the links between the strains, including the description of the L3B3Sb3Gp1SbGp1 genotype, never reported so far for contemporary strains. These results suggest different glanders introduction events in Brazil, including a potential link with strains of European origin, related to colonization or trade. |
Palavras-Chave: |
PCR-HRM; WGS. |
Thesaurus Nal: |
Burkholderia mallei; Genotyping; Horses. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232845/1/2022-Genoma-Burkholkderia-mallei.pdf
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Marc: |
LEADER 02014naa a2200289 a 4500 001 2141175 005 2022-03-22 008 2022 bl uuuu u00u1 u #d 100 1 $aFALCÃO, M. V. D. 245 $aMolecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017).$h[electronic resource] 260 $c2022 520 $aGlanders is an infectious zoonosis caused by Burkholderia (B.) mallei that mainly affects equids. The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction?high-resolution melting (PCRHRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe and the Middle East. Whole genome sequencing of two of these strains (UFAL2 and BM_campo 2.1), belonging to different branches, confirmed the HRM typing results and refined the links between the strains, including the description of the L3B3Sb3Gp1SbGp1 genotype, never reported so far for contemporary strains. These results suggest different glanders introduction events in Brazil, including a potential link with strains of European origin, related to colonization or trade. 650 $aBurkholderia mallei 650 $aGenotyping 650 $aHorses 653 $aPCR-HRM 653 $aWGS 700 1 $aLAROUCAU, K. 700 1 $aVORIMORE, F. 700 1 $aDESHAYES, T. 700 1 $aSANTANA, V. L. A. 700 1 $aSILVA, K. P. C. 700 1 $aNASCIMENTO, S. A. DO 700 1 $aCASTRO, R. S. DE 700 1 $aARAUJO, F. R. 700 1 $aMOTA, R. A. 773 $tInfection, Genetics and Evolution$gv. 99, 2022.
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Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
08/06/2020 |
Data da última atualização: |
12/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
GARCEZ, B. S.; ALVES, A. A.; OLIVEIRA, M. E. de; MOREIRA FILHO, M. A.; AZEVEDO, D. M. M. R.; LACERDA, M. da S. B. |
Afiliação: |
Bruno Spindola Garcez, IFCE; Arnaud Azevêdo Alves, UFPI; Maria Elizabete de Oliveira, UFPI; Miguel Arcanjo Moreira Filho, UFPI; DANIELLE MARIA MACHADO R AZEVEDO, CPAMN; Marlúcia da Silva Bezerra Lacerda, IFCE. |
Título: |
Nutrient metabolism and ingestive behavior of goats fed diets containing palm tree fruit. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Revista Ciência Agronômica, v. 51, n. 2, e20196608, 2020. |
ISSN: |
1806-6690 |
DOI: |
10.5935/1806-6690.20200029 |
Idioma: |
Inglês |
Conteúdo: |
The objective was to evaluate the nutrient metabolism and ingestive behavior of goats fed diets containing fruits of Carnauba and Tucum palm trees, abundant in the Northeast region with fruiting in the dry season and use as alternative food for ruminants. For this end, 21 goats fed three diets, one control and two with carnauba or tucum fruits, in a completely randomized design. We determined nutrient intake through total collection of leftovers, faces and urine, as well as energy and nitrogen balance. Ingestive behavior was assessed by visual observations every 5 min for 24 h. There was a reduction in dry matter intake of 0.183 and 0.223 kg/day for diets containing tucum and carnauba fruits, respectively. The intake of digestible protein (78.04 gDP/day) and metabolizable energy (2.51 McalME/day) of the diet containing tucum fruits met the nutritional requirements of the animals, besides resulting in nitrogen balance above 60% and increase of 0.57 Mcal/kgDM of digestible energy of the diets. Diets containing fruits of carnauba required a longer rumination (453.65 min/day), associated with the higher fiber content in their composition. The inclusion of carnauba and tucum fruits in diets composed of corn, soybean and Tifton 85 hay for growing goats promotes a reduction in dry matter intake due to the low quality of the fiber of these fruits. However, the diets containing tucum fruits met the nutritional requirements of goats regarding digestible protein and metabolizable energy, suggesting the use of this alternative food for this category. MenosThe objective was to evaluate the nutrient metabolism and ingestive behavior of goats fed diets containing fruits of Carnauba and Tucum palm trees, abundant in the Northeast region with fruiting in the dry season and use as alternative food for ruminants. For this end, 21 goats fed three diets, one control and two with carnauba or tucum fruits, in a completely randomized design. We determined nutrient intake through total collection of leftovers, faces and urine, as well as energy and nitrogen balance. Ingestive behavior was assessed by visual observations every 5 min for 24 h. There was a reduction in dry matter intake of 0.183 and 0.223 kg/day for diets containing tucum and carnauba fruits, respectively. The intake of digestible protein (78.04 gDP/day) and metabolizable energy (2.51 McalME/day) of the diet containing tucum fruits met the nutritional requirements of the animals, besides resulting in nitrogen balance above 60% and increase of 0.57 Mcal/kgDM of digestible energy of the diets. Diets containing fruits of carnauba required a longer rumination (453.65 min/day), associated with the higher fiber content in their composition. The inclusion of carnauba and tucum fruits in diets composed of corn, soybean and Tifton 85 hay for growing goats promotes a reduction in dry matter intake due to the low quality of the fiber of these fruits. However, the diets containing tucum fruits met the nutritional requirements of goats regarding digestible protein and metabolizable energ... Mostrar Tudo |
Palavras-Chave: |
Bactris setosa; Copernicia prunifera. |
Thesagro: |
Alimento Alternativo; Balanço de Energia; Matéria Seca. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213842/1/NutrientMetabolismIngestiveGoatsRCA2020.pdf
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Marc: |
LEADER 02379naa a2200265 a 4500 001 2123146 005 2020-08-12 008 2020 bl uuuu u00u1 u #d 022 $a1806-6690 024 7 $a10.5935/1806-6690.20200029$2DOI 100 1 $aGARCEZ, B. S. 245 $aNutrient metabolism and ingestive behavior of goats fed diets containing palm tree fruit.$h[electronic resource] 260 $c2020 520 $aThe objective was to evaluate the nutrient metabolism and ingestive behavior of goats fed diets containing fruits of Carnauba and Tucum palm trees, abundant in the Northeast region with fruiting in the dry season and use as alternative food for ruminants. For this end, 21 goats fed three diets, one control and two with carnauba or tucum fruits, in a completely randomized design. We determined nutrient intake through total collection of leftovers, faces and urine, as well as energy and nitrogen balance. Ingestive behavior was assessed by visual observations every 5 min for 24 h. There was a reduction in dry matter intake of 0.183 and 0.223 kg/day for diets containing tucum and carnauba fruits, respectively. The intake of digestible protein (78.04 gDP/day) and metabolizable energy (2.51 McalME/day) of the diet containing tucum fruits met the nutritional requirements of the animals, besides resulting in nitrogen balance above 60% and increase of 0.57 Mcal/kgDM of digestible energy of the diets. Diets containing fruits of carnauba required a longer rumination (453.65 min/day), associated with the higher fiber content in their composition. The inclusion of carnauba and tucum fruits in diets composed of corn, soybean and Tifton 85 hay for growing goats promotes a reduction in dry matter intake due to the low quality of the fiber of these fruits. However, the diets containing tucum fruits met the nutritional requirements of goats regarding digestible protein and metabolizable energy, suggesting the use of this alternative food for this category. 650 $aAlimento Alternativo 650 $aBalanço de Energia 650 $aMatéria Seca 653 $aBactris setosa 653 $aCopernicia prunifera 700 1 $aALVES, A. A. 700 1 $aOLIVEIRA, M. E. de 700 1 $aMOREIRA FILHO, M. A. 700 1 $aAZEVEDO, D. M. M. R. 700 1 $aLACERDA, M. da S. B. 773 $tRevista Ciência Agronômica$gv. 51, n. 2, e20196608, 2020.
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