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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/01/2011 |
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Data da última atualização: |
19/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALICENTE, F. H.; TUELHER, E. de S.; LEITE, M. I. S.; FREIRE, F. L.; VIEIRA, C. M. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 9, n. 1, p. 1-11, 2010. |
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DOI: |
http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11 |
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Idioma: |
Inglês |
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Conteúdo: |
Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations. MenosBacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 1... Mostrar Tudo |
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Palavras-Chave: |
Biocontrole; Inseticida microbiano. |
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Thesagro: |
Inseticida; Praga de planta; Spodoptera Frugiperda. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26116/1/Valicente.pdf
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Marc: |
LEADER 02501naa a2200241 a 4500
001 1874936
005 2017-05-19
008 2010 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11$2DOI
100 1 $aVALICENTE, F. H.
245 $aProduction of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.$h[electronic resource]
260 $c2010
520 $aBacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations.
650 $aInseticida
650 $aPraga de planta
650 $aSpodoptera Frugiperda
653 $aBiocontrole
653 $aInseticida microbiano
700 1 $aTUELHER, E. de S.
700 1 $aLEITE, M. I. S.
700 1 $aFREIRE, F. L.
700 1 $aVIEIRA, C. M.
773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 9, n. 1, p. 1-11, 2010.
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