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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
18/12/2015 |
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Data da última atualização: |
03/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MERTZ-HENNING, L. M.; NAGASHIMA, A. I.; KRZYZANOWSKI, F. C.; BINNECK, E.; HENNING, F. A. |
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Afiliação: |
LILIANE MARCIA MERTZ HENNING, CNPSO; FRANCISCO CARLOS KRZYZANOWSKI, CNPSO; ELISEU BINNECK, CNPSO; FERNANDO AUGUSTO HENNING, CNPSO. |
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Título: |
Relative quantification of gene expression levels associated with lignin biosynthesis in soybean seed coat. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Seed Science and Technology, Zurich, v. 43, n. 3, p. 445-455, Dec. 2015. |
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ISSN: |
0251-0952 |
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DOI: |
10.15258/sst.2015.43.3.13 |
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Idioma: |
Inglês |
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Conteúdo: |
There is genetic variability among soybean genotypes for seed quality, which may be related to seed coat characteristics. An example of this is the variable lignin content in the seed coat, a trait associated with tolerance to mechanical damage and to deterioration caused by high relative humidity. The aim of this study was to evaluate soybean seed quality and determine the relative expression of genes associated with lignin biosynthesis in soybean seed coats. Two soybean cultivars differing with respect to lignin content in the seed coat, Doko (high) and Savana (low) were studied. Evaluations were carried out on seeds of four different sizes, representing the development stages of the seeds. Seed quality was evaluated by germination, accelerated ageing and electrical conductivity tests. Through real-time PCR, relative quantification (Doko/Savana) of the genes that encode PAL, C4H, 4CL, HCT, CCoAOMT, CCR, COMT and CAD enzymes was carried out, using the ?-Actin gene as an endogenous control. According to the results of the electrical conductivity test, Doko cultivar with high lignin content has the best seed coat structure. PAL, CCoA, 4Cl and CCR genes were upregulated in Doko cultivar relative to the Savana cultivar. |
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Thesagro: |
Genótipo; Qualidade; Semente; Soja. |
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Thesaurus NAL: |
Genotype; Seed quality; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02046naa a2200277 a 4500
001 2031997
005 2017-11-03
008 2015 bl uuuu u00u1 u #d
022 $a0251-0952
024 7 $a10.15258/sst.2015.43.3.13$2DOI
100 1 $aMERTZ-HENNING, L. M.
245 $aRelative quantification of gene expression levels associated with lignin biosynthesis in soybean seed coat.$h[electronic resource]
260 $c2015
520 $aThere is genetic variability among soybean genotypes for seed quality, which may be related to seed coat characteristics. An example of this is the variable lignin content in the seed coat, a trait associated with tolerance to mechanical damage and to deterioration caused by high relative humidity. The aim of this study was to evaluate soybean seed quality and determine the relative expression of genes associated with lignin biosynthesis in soybean seed coats. Two soybean cultivars differing with respect to lignin content in the seed coat, Doko (high) and Savana (low) were studied. Evaluations were carried out on seeds of four different sizes, representing the development stages of the seeds. Seed quality was evaluated by germination, accelerated ageing and electrical conductivity tests. Through real-time PCR, relative quantification (Doko/Savana) of the genes that encode PAL, C4H, 4CL, HCT, CCoAOMT, CCR, COMT and CAD enzymes was carried out, using the ?-Actin gene as an endogenous control. According to the results of the electrical conductivity test, Doko cultivar with high lignin content has the best seed coat structure. PAL, CCoA, 4Cl and CCR genes were upregulated in Doko cultivar relative to the Savana cultivar.
650 $aGenotype
650 $aSeed quality
650 $aSoybeans
650 $aGenótipo
650 $aQualidade
650 $aSemente
650 $aSoja
700 1 $aNAGASHIMA, A. I.
700 1 $aKRZYZANOWSKI, F. C.
700 1 $aBINNECK, E.
700 1 $aHENNING, F. A.
773 $tSeed Science and Technology, Zurich$gv. 43, n. 3, p. 445-455, Dec. 2015.
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