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Registros recuperados : 64 | |
41. | | QUIRINO, B. F.; BERGMAN, J. C.; COSTA, O. Y. A.; PALUAN, S. G.; RAMOS, N. S. P. L.; SOUTO, B. de M.; BARRETO, C. C.; KRUGER, R. H. Identification of glycosyl hydrolases in the microbial biodiversity of Brazil using a metagenomic approach. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 34., 2012, New Orleans. [Anais...]. Fairfax, VA: Society for Industrial Microbiology & Biotechnology, 2012. Não paginado. Poster Session 1: 7-44. Biblioteca(s): Embrapa Agroenergia. |
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42. | | ASSIS, O. Y. de; SOUTO, B. de M.; DOMENECH, D.; CARVALHO B. J.; KRUGER, R. H.; KYAM, C. M.; CHAVES B. C.; QUIRINO, B. F. Culture-independent assessment of the microbial diversity in the sugarcane-ethanol production process. Hechos Microbiológicos, v. 5, n. 2, suplemento 2, p. 87, 2014. p. 134. Trabalho apresentado no XXII Congresso Latinoamericano de Microbiología - ALAM 2014 e 4 Congresso Colombiano de Microbiología - 4 CCM 2014. Biblioteca(s): Embrapa Agroenergia. |
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43. | | QUIRINO, B. F.; BERGMANN, J. C.; RAMOS, N. S. P. L.; PALUAN, S. de F.; SOUTO, B. de M.; RODRIGUES, L. P.; BARRETO, C. C.; KRUGER, R. H. Colocando a biodiversidade microbiana brasileira a serviço da indústria de biocombustíveis. In: SIMPÓSIO MICRORGANISMOS EM AGROENERGIAS, 2012, Brasília, DF. Da prospecção aos bioprocessos: anais. Brasília, DF: Embrapa Agroenergia, 2013. (Embrapa Agroenergia. Documentos, 15). p. 23-30. Biblioteca(s): Embrapa Agroenergia. |
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44. | | QUIRINO, B. F.; CUNHA, I. S.; SOUTO, B. de M.; CARVALHO, L. S.; RAMOS, N. S. P. L.; COSTA, O. Y. A.; BARRETO, C. C.; KRUGER, R. H. Characterization of the bacterial diversity in the goat rumen and identification of enzymes with potential use in the biofuels industry. In: ANNUAL MEETING AND EXHIBITION, 60., 2010, San Francisco, CA. Industrial microbiology and biotechnology. [S.l]: Society for Industrial Microbiology, 2010. p. 107 Poster abstract P9. Biblioteca(s): Embrapa Agroenergia. |
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45. | | PALUAN, S. F.; SILVA, R. B.; BITENCOURT, A. C. A.; SANTOS, D. F. K.; SOUTO, B. de M.; KRUGER, R. H.; QUIRINO, B. F. Characterization of hydrolytic enzymes isolated from an Amazon soil environmental DNA library. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 68. Biblioteca(s): Embrapa Agroenergia. |
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46. | | CUNHA, I. S.; MÁXIMO, R. M.; CAMPOS, P. F.; NORONHA, E. F.; BOMFIM, M. A. D.; KRÜGER, R. H.; QUIRINO, B. F. Construção e screening de uma biblioteca metagenômica de rúmen de caprinos. In: WORKSHOP EM CIÊNCIAS GENÔMICAS E BIOTECNOLOGIA, 1., 2009, Brasília, DF. [Anais...]. Brasília, DF: [UCB], 2009. Não paginado Biblioteca(s): Embrapa Agroenergia. |
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47. | | BERGMANN, J. C.; COSTA, O. Y. A.; RAMOS, N. S. P. L.; FURTADO, R. S.; KRUGER, R. H.; QUIRINO, B. F. Construction of metagenomic libraries with large inserts of DNA from the Amazonian soil microbiota and screening for enzymes with biotechnological potential. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 64-65. Biblioteca(s): Embrapa Agroenergia. |
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48. | | CÂNDIDO, E. S.; SILVA, R. B.; AQUINO, E. N.; NORONHA, E. F.; FRANCO, O. L.; KRÜGER, R. H.; FONTES, W.; QUIRINO, B. F. Analysis of the Arabidopsis thaliana - Xanthomonas campestris pv. campestris pathosystem using proteomics tools. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28., 2008; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12., 2008; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., 2008; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR: UEL, 2008. Biblioteca(s): Embrapa Agroenergia. |
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49. | | CUNHA, I. S.; BARRETO, C. C.; COSTA, O. Y. A.; BOMFIM, M. A. D.; CASTRO, A. P. de; KRUGER, R. H.; QUIRINO, B. F. Bacteria and Archaea community structure in the rumen microbiome of goats (Capra hircus) from the semiarid region of Brazil. Anaerobe, London, v. 17, n. 3, p. 118-124, Jul. 2011. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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50. | | COSTA, O. Y. A.; SOUTO, B. de M.; TUPINAMBA, D. D.; BERGMANN, J. C.; KRUGER, R. H.; KYAW, C. M.; BARRETO, C. C.; QUIRINO, B. F. Avaliação da diversidade microbiana do processo de produção de etanol de cana-de-açúcar por meio de abordagem independente de cultivo. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. 111 - 112 Biblioteca(s): Embrapa Agroenergia. |
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51. | | CÃNDIDO, E. S.; CAMPOS, P. F.; PEREIRA, J. L.; SANTANA, B. G.; SILVA, R. B.; QUEZADO-DURVAL, A. M.; NORONHA, E. F.; KRÜGER, R. H.; QUIRINO, B. F. Caracterização e análise da atividade exoenzimática de Xanthomonas gardneri na presença de tecido vegetal. Fitopatologia Brasileira, Brasília, DF, v. 32, ago. 2007. s 263. Suplemento. Trabalho apresentado no 49. Congresso Brasileiro de Fitopatologia, 2007, Maringá, PR. Resumo n. 769. Biblioteca(s): Embrapa Hortaliças. |
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52. | | CUNHA, I. S.; FREIRE, M. S.; LEMOS, T. O.; BARRETO, C. C.; QUIRINO, B. F.; FRANCO, O. L.; KRUGER, R. H. Prokaryotic diversity present in the semi-arid goat rumen and its biotechnological potential. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010. Não paginado. Disponível em: < http://www.sbbq.org.br/2010/cdrom/busca.htm?query=quirino%2C+b.+f. >. Acesso em: 16 ago. 2010. Biblioteca(s): Embrapa Agroenergia. |
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53. | | GUIMARÃES, H. I. P.; SANTANA, R. H.; SILVEIRA, R.; PINTO, O. H. B.; QUIRINO, B. F.; BARRETO, C. C.; BUSTAMANTE, M. M. da C.; KRÜGER, R. H. Seasonal Variations in Soil Microbiota Profile of Termite (Syntermes wheeleri) Mounds in the Brazilian Tropical Savanna. Microorganisms, v. 8, 1482, 2020. 8 Special Issue: Soil Microbiome: Biotic and Abiotic Interactions. Biblioteca(s): Embrapa Agroenergia. |
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54. | | GOMES, P. H. M.; CÂNDIDO, E. S.; AQUINO, E. N.; NORONHA, E. F.; FRANCO, O. L.; FONTES, W.; KRÜGER, R. H.; QUIRINO, B. F. Xanthomonas campestris pv. campestris secretome toward susceptible and resistant Arabidopsis thaliana ecotypes. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28., 2008; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12., 2008; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., 2008; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR: UEL, 2008. Biblioteca(s): Embrapa Agroenergia. |
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55. | | QUIRINO, B. F.; BERGMANN, J. C.; SOUTO, B. de M.; TUPINAMBA, D.; RAMOS, T. G.; SCHROEDER, L. F.; PERIM, L.; NORONHA, E. F.; BARRETO, C. C.; KRUGER, R. H. Metagenomic glycosyl hydrolases from the Amazon soil and goat rumen. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014. Biblioteca(s): Embrapa Agroenergia. |
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56. | | PALUAN, S. P.; SILVA, R. B.; CARVALHO, L. S.; SANTOS, D. F. K.; RAMOS, N. S. P. L.; BERGMAN, J. C.; NORONHA, E. F.; KRUGER, R. H.; QUIRINO, B. F. Enzymes of biotechnological interest prospected from a small insert metagenomic library constructed with Amazon soil microbial DNA. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010. Não paginado. Disponível em: < http://www.sbbq.org.br/2010/cdrom/busca.htm?query=quirino%2C+b.+f. >. Acesso em: 16 ago. 2010. Biblioteca(s): Embrapa Agroenergia. |
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57. | | ARAÚJO, J. F.; CASTRO, A. R. de; COSTA, M. M. do C.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; QUIRINO, B. F.; BUSTAMANTE, M. M. C.; WILLIAMSON, L.; HANDELSMAN, J.; KRUGER, R. H. Characterization of soil bacterial assemblies in Brazilian Savanna-Like vegetation reveals acidobacteria dominance. Microbial Ecology, v. 64, p. 760-770, 2012. Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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58. | | SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F. Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase. PLoS ONE, v. 16, n. 1, e024511, 2021. Biblioteca(s): Embrapa Agroenergia. |
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59. | | LIMA, R. A. T.; OLIVEIRA, G. d.; SOUZA, A. A.; LOPES, F. A. C.; SANTANA, R. H.; ISTVAN, P.; QUIRINO, B. F.; BARBOSA, J.; FREITAS, S. d.; GARAY, A. V.; KRUGER, R. H. Functional and structural characterization of a novel GH3 Beta-glucosidase from the gut metagenome of the Brazilian Cerrado termite Syntermes wheeleri. International Journal of Biological Macromolecules, v. 165, Part A, p. 822-834, Dec. 2020. 165 Biblioteca(s): Embrapa Agroenergia. |
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60. | | CASTRO, A. P. de; ARAÚJO JÚNIOR, S. D.; REIS, A. M. M.; MOURA, R. L.; FRANCINI FILHO, R. B.; PAPPAS JUNIOR, G. J.; RODRIGUES, T. B.; THOMPSON, F. L.; KRÜGER, R. H. Bacterial community associated with healthy and diseased reef coral Mussismilia hispida from Eastern Brazil. Microbial Ecology, v. 59, p. 658-667, 2010. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 64 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
15/09/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. |
Afiliação: |
ISIS VIANA MENDES, Universidade de Brasília; MARIANA BOTELHO GARCIA, Universidade Católica de Brasília; ANA CAROLINA ARAUJO BITENCOURT, Universidade de Brasília; RENATA HENRIQUE SANTANA, Instituto Federal de Brasília; PHILIPPE DE CASTRO LINS, Universidade de Brasília; RAFAELLA SILVEIRA, Universidade de Brasília; BLAKE A. SIMMONS, Joint BioEnergy Institute; JOHN M. GLADDEN, Joint BioEnergy Institute; RICARDO HENRIQUE KRUGER, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
Título: |
Bacterial diversity dynamics in microbial consortia selected for lignin utilization. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
PLoS ONE, v. 16, n. 9, e0255083, 2021. |
Descrição Física: |
PDF: il. color. |
DOI: |
https://doi.org/10.1371/journal.pone.0255083 |
Idioma: |
Inglês |
Conteúdo: |
Abstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications. MenosAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lign... Mostrar Tudo |
Thesagro: |
Biotecnologia; Carbono; Composto Fenólico; Lignina. |
Thesaurus NAL: |
Application technology; Biotechnology; Carbon; Degradation; Energy conversion; Lignin; Phenolic compounds. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226045/1/Bacterial-diversity-dynamics-in-microbial-2021.pdf
|
Marc: |
LEADER 03127naa a2200385 a 4500 001 2134421 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1371/journal.pone.0255083$2DOI 100 1 $aMENDES, I. V. 245 $aBacterial diversity dynamics in microbial consortia selected for lignin utilization.$h[electronic resource] 260 $c2021 300 $cPDF: il. color. 520 $aAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications. 650 $aApplication technology 650 $aBiotechnology 650 $aCarbon 650 $aDegradation 650 $aEnergy conversion 650 $aLignin 650 $aPhenolic compounds 650 $aBiotecnologia 650 $aCarbono 650 $aComposto Fenólico 650 $aLignina 700 1 $aGARCIA, M. B. 700 1 $aBITENCOURT, A. C. A. 700 1 $aSANTANA, R. H. 700 1 $aLINS, P. de C. 700 1 $aSILVEIRA, R. 700 1 $aSIMMONS, B. A. 700 1 $aGLADDEN, J. M. 700 1 $aKRUGER, R. H. 700 1 $aQUIRINO, B. F. 773 $tPLoS ONE$gv. 16, n. 9, e0255083, 2021.
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