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| Registros recuperados : 64 | |
| 41. |  | QUIRINO, B. F.; BERGMAN, J. C.; COSTA, O. Y. A.; PALUAN, S. G.; RAMOS, N. S. P. L.; SOUTO, B. de M.; BARRETO, C. C.; KRUGER, R. H. Identification of glycosyl hydrolases in the microbial biodiversity of Brazil using a metagenomic approach. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 34., 2012, New Orleans. [Anais...]. Fairfax, VA: Society for Industrial Microbiology & Biotechnology, 2012. Não paginado. Poster Session 1: 7-44.| Biblioteca(s): Embrapa Agroenergia. |
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| 42. | | ASSIS, O. Y. de; SOUTO, B. de M.; DOMENECH, D.; CARVALHO B. J.; KRUGER, R. H.; KYAM, C. M.; CHAVES B. C.; QUIRINO, B. F. Culture-independent assessment of the microbial diversity in the sugarcane-ethanol production process. Hechos Microbiológicos, v. 5, n. 2, suplemento 2, p. 87, 2014. p. 134. Trabalho apresentado no XXII Congresso Latinoamericano de Microbiología - ALAM 2014 e 4 Congresso Colombiano de Microbiología - 4 CCM 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 43. | | QUIRINO, B. F.; BERGMANN, J. C.; RAMOS, N. S. P. L.; PALUAN, S. de F.; SOUTO, B. de M.; RODRIGUES, L. P.; BARRETO, C. C.; KRUGER, R. H. Colocando a biodiversidade microbiana brasileira a serviço da indústria de biocombustíveis. In: SIMPÓSIO MICRORGANISMOS EM AGROENERGIAS, 2012, Brasília, DF. Da prospecção aos bioprocessos: anais. Brasília, DF: Embrapa Agroenergia, 2013. (Embrapa Agroenergia. Documentos, 15). p. 23-30.| Biblioteca(s): Embrapa Agroenergia. |
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| 44. | | QUIRINO, B. F.; CUNHA, I. S.; SOUTO, B. de M.; CARVALHO, L. S.; RAMOS, N. S. P. L.; COSTA, O. Y. A.; BARRETO, C. C.; KRUGER, R. H. Characterization of the bacterial diversity in the goat rumen and identification of enzymes with potential use in the biofuels industry. In: ANNUAL MEETING AND EXHIBITION, 60., 2010, San Francisco, CA. Industrial microbiology and biotechnology. [S.l]: Society for Industrial Microbiology, 2010. p. 107 Poster abstract P9.| Biblioteca(s): Embrapa Agroenergia. |
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| 45. | | PALUAN, S. F.; SILVA, R. B.; BITENCOURT, A. C. A.; SANTOS, D. F. K.; SOUTO, B. de M.; KRUGER, R. H.; QUIRINO, B. F. Characterization of hydrolytic enzymes isolated from an Amazon soil environmental DNA library. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 68.| Biblioteca(s): Embrapa Agroenergia. |
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| 46. | | CUNHA, I. S.; MÁXIMO, R. M.; CAMPOS, P. F.; NORONHA, E. F.; BOMFIM, M. A. D.; KRÜGER, R. H.; QUIRINO, B. F. Construção e screening de uma biblioteca metagenômica de rúmen de caprinos. In: WORKSHOP EM CIÊNCIAS GENÔMICAS E BIOTECNOLOGIA, 1., 2009, Brasília, DF. [Anais...]. Brasília, DF: [UCB], 2009. Não paginado| Biblioteca(s): Embrapa Agroenergia. |
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| 47. | | BERGMANN, J. C.; COSTA, O. Y. A.; RAMOS, N. S. P. L.; FURTADO, R. S.; KRUGER, R. H.; QUIRINO, B. F. Construction of metagenomic libraries with large inserts of DNA from the Amazonian soil microbiota and screening for enzymes with biotechnological potential. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 64-65.| Biblioteca(s): Embrapa Agroenergia. |
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| 48. | | CÂNDIDO, E. S.; SILVA, R. B.; AQUINO, E. N.; NORONHA, E. F.; FRANCO, O. L.; KRÜGER, R. H.; FONTES, W.; QUIRINO, B. F. Analysis of the Arabidopsis thaliana - Xanthomonas campestris pv. campestris pathosystem using proteomics tools. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28., 2008; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12., 2008; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., 2008; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR: UEL, 2008.| Biblioteca(s): Embrapa Agroenergia. |
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| 49. | | CUNHA, I. S.; BARRETO, C. C.; COSTA, O. Y. A.; BOMFIM, M. A. D.; CASTRO, A. P. de; KRUGER, R. H.; QUIRINO, B. F. Bacteria and Archaea community structure in the rumen microbiome of goats (Capra hircus) from the semiarid region of Brazil. Anaerobe, London, v. 17, n. 3, p. 118-124, Jul. 2011.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 50. | | COSTA, O. Y. A.; SOUTO, B. de M.; TUPINAMBA, D. D.; BERGMANN, J. C.; KRUGER, R. H.; KYAW, C. M.; BARRETO, C. C.; QUIRINO, B. F. Avaliação da diversidade microbiana do processo de produção de etanol de cana-de-açúcar por meio de abordagem independente de cultivo. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. 111 - 112| Biblioteca(s): Embrapa Agroenergia. |
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| 51. | | CÃNDIDO, E. S.; CAMPOS, P. F.; PEREIRA, J. L.; SANTANA, B. G.; SILVA, R. B.; QUEZADO-DURVAL, A. M.; NORONHA, E. F.; KRÜGER, R. H.; QUIRINO, B. F. Caracterização e análise da atividade exoenzimática de Xanthomonas gardneri na presença de tecido vegetal. Fitopatologia Brasileira, Brasília, DF, v. 32, ago. 2007. s 263. Suplemento. Trabalho apresentado no 49. Congresso Brasileiro de Fitopatologia, 2007, Maringá, PR. Resumo n. 769.| Biblioteca(s): Embrapa Hortaliças. |
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| 52. | | CUNHA, I. S.; FREIRE, M. S.; LEMOS, T. O.; BARRETO, C. C.; QUIRINO, B. F.; FRANCO, O. L.; KRUGER, R. H. Prokaryotic diversity present in the semi-arid goat rumen and its biotechnological potential. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010. Não paginado. Disponível em: < http://www.sbbq.org.br/2010/cdrom/busca.htm?query=quirino%2C+b.+f. >. Acesso em: 16 ago. 2010.| Biblioteca(s): Embrapa Agroenergia. |
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| 53. | | GUIMARÃES, H. I. P.; SANTANA, R. H.; SILVEIRA, R.; PINTO, O. H. B.; QUIRINO, B. F.; BARRETO, C. C.; BUSTAMANTE, M. M. da C.; KRÜGER, R. H. Seasonal Variations in Soil Microbiota Profile of Termite (Syntermes wheeleri) Mounds in the Brazilian Tropical Savanna. Microorganisms, v. 8, 1482, 2020. 8 Special Issue: Soil Microbiome: Biotic and Abiotic Interactions.| Biblioteca(s): Embrapa Agroenergia. |
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| 54. | | GOMES, P. H. M.; CÂNDIDO, E. S.; AQUINO, E. N.; NORONHA, E. F.; FRANCO, O. L.; FONTES, W.; KRÜGER, R. H.; QUIRINO, B. F. Xanthomonas campestris pv. campestris secretome toward susceptible and resistant Arabidopsis thaliana ecotypes. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28., 2008; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12., 2008; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., 2008; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR: UEL, 2008.| Biblioteca(s): Embrapa Agroenergia. |
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| 55. | | QUIRINO, B. F.; BERGMANN, J. C.; SOUTO, B. de M.; TUPINAMBA, D.; RAMOS, T. G.; SCHROEDER, L. F.; PERIM, L.; NORONHA, E. F.; BARRETO, C. C.; KRUGER, R. H. Metagenomic glycosyl hydrolases from the Amazon soil and goat rumen. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 56. | | PALUAN, S. P.; SILVA, R. B.; CARVALHO, L. S.; SANTOS, D. F. K.; RAMOS, N. S. P. L.; BERGMAN, J. C.; NORONHA, E. F.; KRUGER, R. H.; QUIRINO, B. F. Enzymes of biotechnological interest prospected from a small insert metagenomic library constructed with Amazon soil microbial DNA. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010. Não paginado. Disponível em: < http://www.sbbq.org.br/2010/cdrom/busca.htm?query=quirino%2C+b.+f. >. Acesso em: 16 ago. 2010.| Biblioteca(s): Embrapa Agroenergia. |
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| 57. | | ARAÚJO, J. F.; CASTRO, A. R. de; COSTA, M. M. do C.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; QUIRINO, B. F.; BUSTAMANTE, M. M. C.; WILLIAMSON, L.; HANDELSMAN, J.; KRUGER, R. H. Characterization of soil bacterial assemblies in Brazilian Savanna-Like vegetation reveals acidobacteria dominance. Microbial Ecology, v. 64, p. 760-770, 2012.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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| 58. | | SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F. Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase. PLoS ONE, v. 16, n. 1, e024511, 2021.| Biblioteca(s): Embrapa Agroenergia. |
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| 59. | | LIMA, R. A. T.; OLIVEIRA, G. d.; SOUZA, A. A.; LOPES, F. A. C.; SANTANA, R. H.; ISTVAN, P.; QUIRINO, B. F.; BARBOSA, J.; FREITAS, S. d.; GARAY, A. V.; KRUGER, R. H. Functional and structural characterization of a novel GH3 Beta-glucosidase from the gut metagenome of the Brazilian Cerrado termite Syntermes wheeleri. International Journal of Biological Macromolecules, v. 165, Part A, p. 822-834, Dec. 2020. 165| Biblioteca(s): Embrapa Agroenergia. |
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| 60. | | CASTRO, A. P. de; ARAÚJO JÚNIOR, S. D.; REIS, A. M. M.; MOURA, R. L.; FRANCINI FILHO, R. B.; PAPPAS JUNIOR, G. J.; RODRIGUES, T. B.; THOMPSON, F. L.; KRÜGER, R. H. Bacterial community associated with healthy and diseased reef coral Mussismilia hispida from Eastern Brazil. Microbial Ecology, v. 59, p. 658-667, 2010.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 64 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
27/01/2021 |
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Data da última atualização: |
27/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F. |
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Afiliação: |
BETULIA DE MORAIS SOUTO, CNPAE; Ana Carolina Bitencourt de Araújo; Pedro Ricardo Vieira Hamann, Universidade de Brasília; Andrêssa de Rezende Bastos; Isabel de Souza Cunha, Universidade Católica de Brasília; Julianna Peixoto, Universidade de Brasília; Ricardo Henrique Kruger, Universidade de Brasília; Eliane Ferreira Noronha, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PLoS ONE, v. 16, n. 1, e024511, 2021. |
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DOI: |
https://doi.org/10.1371/journal.pone.0245118 |
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Idioma: |
Inglês |
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Conteúdo: |
Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol. MenosFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetical... Mostrar Tudo |
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Thesagro: |
Clonagem; Enzima; Peptídeo; Programa de Computador. |
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Thesaurus NAL: |
Cloning (animals); Computer software; Enzymes; Metagenomics; Prevotella; Screening; Signal peptide. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220708/1/Functional-screening-of-a-Caatinga-goat-2021.pdf
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Marc: |
LEADER 02852naa a2200361 a 4500
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024 7 $ahttps://doi.org/10.1371/journal.pone.0245118$2DOI
100 1 $aSOUTO, B. de M.
245 $aFunctional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase.$h[electronic resource]
260 $c2021
520 $aFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol.
650 $aCloning (animals)
650 $aComputer software
650 $aEnzymes
650 $aMetagenomics
650 $aPrevotella
650 $aScreening
650 $aSignal peptide
650 $aClonagem
650 $aEnzima
650 $aPeptídeo
650 $aPrograma de Computador
700 1 $aARAÚJO, A. C. B. de
700 1 $aHAMANN, P. R. V.
700 1 $aBASTOS, A. de R.
700 1 $aCUNHA, I. de S.
700 1 $aPEIXOTO, J.
700 1 $aKRUGER, R. H.
700 1 $aNORONHA, E. F.
700 1 $aQUIRINO, B. F.
773 $tPLoS ONE$gv. 16, n. 1, e024511, 2021.
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Embrapa Agroenergia (CNPAE) |
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