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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
12/03/1999 |
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Data da última atualização: |
11/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, I. R. P.; MacADAM, J. W. |
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Afiliação: |
ISABEL REGINA PRAZERES DE SOUZA, CNPMS. |
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Título: |
A transient increase in apoplastic peroxidase activity precedes decrease in elongation rate of B73 maize (Zea mays) leaf blades. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Physiologia Plantarum, Copenhagen, v. 104, n. 4, p. 556-562, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Peroxidase (EC 1.11.1.7) activity from homogenized tissue or in apoplastic fluid was analyzed along the developmental gradient of expanding B73 maize (Zea maus L.) leaf blades. Soluble plus ionically bound peroxidase activity from homogenized tissue was present in high levels at the leaf base, which includes the region of cell division, and decreased as tissue was displaced away from the base by growth. A different pattern of change in peroxidase activity was seen in apoplastic fluid extracted from segments of intact tissue, where an increase in peroxidase activity preceded a rapid decrease in leaf elongation rate. Similar patterns in peroxidase activity from homogenized and intact tissue have been found in leaf blades of tall fescuse (Festuca arundinacea Schreb), suggesting a common phenomenon. At the location within the elongation zone where the increase in apoplastic peroxidase activity occurred, the activities of neutral and acidic (pl 4.6) peroxidase isoforms were also elevated in both the homogenate and in apoplastic fluid. The coincidence of these isoforms with the decline in leaf elongation rate suggests they may contribute to cessation of growth. At the distal end of the elongation zone. The activities of other acidic peroxidases (pl 5.6 and 5.7) increased in the homogenate and in apoplastic fluid, and remained elevated as tissue was displaced into the maturation region. The location of their appearance and their relatively high activity in the maturation region suggest the involvement of these isoforms in lignification. MenosPeroxidase (EC 1.11.1.7) activity from homogenized tissue or in apoplastic fluid was analyzed along the developmental gradient of expanding B73 maize (Zea maus L.) leaf blades. Soluble plus ionically bound peroxidase activity from homogenized tissue was present in high levels at the leaf base, which includes the region of cell division, and decreased as tissue was displaced away from the base by growth. A different pattern of change in peroxidase activity was seen in apoplastic fluid extracted from segments of intact tissue, where an increase in peroxidase activity preceded a rapid decrease in leaf elongation rate. Similar patterns in peroxidase activity from homogenized and intact tissue have been found in leaf blades of tall fescuse (Festuca arundinacea Schreb), suggesting a common phenomenon. At the location within the elongation zone where the increase in apoplastic peroxidase activity occurred, the activities of neutral and acidic (pl 4.6) peroxidase isoforms were also elevated in both the homogenate and in apoplastic fluid. The coincidence of these isoforms with the decline in leaf elongation rate suggests they may contribute to cessation of growth. At the distal end of the elongation zone. The activities of other acidic peroxidases (pl 5.6 and 5.7) increased in the homogenate and in apoplastic fluid, and remained elevated as tissue was displaced into the maturation region. The location of their appearance and their relatively high activity in the maturation region sug... Mostrar Tudo |
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Palavras-Chave: |
Growth; Leaf; Maize; Zimograma; Zymogram. |
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Thesagro: |
Crescimento; Folha; Milho; Peroxidase; Zea Mays. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02270naa a2200253 a 4500
001 1482076
005 2018-06-11
008 1998 bl uuuu u00u1 u #d
100 1 $aSOUZA, I. R. P.
245 $aA transient increase in apoplastic peroxidase activity precedes decrease in elongation rate of B73 maize (Zea mays) leaf blades.$h[electronic resource]
260 $c1998
520 $aPeroxidase (EC 1.11.1.7) activity from homogenized tissue or in apoplastic fluid was analyzed along the developmental gradient of expanding B73 maize (Zea maus L.) leaf blades. Soluble plus ionically bound peroxidase activity from homogenized tissue was present in high levels at the leaf base, which includes the region of cell division, and decreased as tissue was displaced away from the base by growth. A different pattern of change in peroxidase activity was seen in apoplastic fluid extracted from segments of intact tissue, where an increase in peroxidase activity preceded a rapid decrease in leaf elongation rate. Similar patterns in peroxidase activity from homogenized and intact tissue have been found in leaf blades of tall fescuse (Festuca arundinacea Schreb), suggesting a common phenomenon. At the location within the elongation zone where the increase in apoplastic peroxidase activity occurred, the activities of neutral and acidic (pl 4.6) peroxidase isoforms were also elevated in both the homogenate and in apoplastic fluid. The coincidence of these isoforms with the decline in leaf elongation rate suggests they may contribute to cessation of growth. At the distal end of the elongation zone. The activities of other acidic peroxidases (pl 5.6 and 5.7) increased in the homogenate and in apoplastic fluid, and remained elevated as tissue was displaced into the maturation region. The location of their appearance and their relatively high activity in the maturation region suggest the involvement of these isoforms in lignification.
650 $aCrescimento
650 $aFolha
650 $aMilho
650 $aPeroxidase
650 $aZea Mays
653 $aGrowth
653 $aLeaf
653 $aMaize
653 $aZimograma
653 $aZymogram
700 1 $aMacADAM, J. W.
773 $tPhysiologia Plantarum, Copenhagen$gv. 104, n. 4, p. 556-562, 1998.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
03/03/2023 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
MACIEL, J. L. N.; KOVALESKI, M.; SILVA, A. N. da; BONATO, A. L. V.; COSTA, I. F. D. da. |
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Afiliação: |
JOAO LEODATO NUNES MACIEL, CNPT; MARCOS KOVALESKI, Bolsista DTI-C; ALIEZE NASCIMENTO DA SILVA, Universidade Federal de Santa Maria; ANA LIDIA VARIANI BONATO, CNPT; IVAN FRANCISCO DRESSLER DA COSTA, Universidade Federal de Santa Maria. |
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Título: |
Occurrence of Pyricularia oryzae Triticum in plants of the genus Urochloa in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Ciência Rural, v. 53, n. 4, 2023. |
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DOI: |
https://doi.org/10.1590/0103-8478cr20210839 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In this study Pyricularia spp., P. oryzae and the P. oryzae pathotype Triticum (PoT) were detected and identified in leaf segments of forage and invasive grasses located in or next to wheat fields. In 2018 and 2019, 66 samples of lesion leaf segments of Urochloa and other grasses were collected in Londrina (PR), Patos de Minas (MG), and Uberaba (MG). The detection and/or identification of the pathogens on the samples was conducted using moist chamber procedures and with the primers MoT3 and Pot2 by PCR. There were DNA amplification with the primer MoT3 (specific for PoT) for 13 (19.69%) of the samples, all of them from Urochloa. The finding that Urochloa hosts PoT at a relatively high rate raises concerns about the importance which these plants may have on the wheat blast cycle as an alternative host for the pathogen and/or source of inoculum for the disease. Resumo: Neste estudo Pyricularia spp., P. oryzae e o patótipo Triticum (PoT) de P. oryzae foram detectados e identificados em segmentos foliares de forrageiras e gramíneas invasoras de lavouras de trigo. Em 2018 e 2019, foram coletadas 66 amostras de segmentos foliares lesionados de Urochloa e outras gramíneas em Londrina (PR), Patos de Minas (MG) e Uberaba (MG). A detecção e/ou identificação dos patógenos nas amostras foi realizada por meio de procedimentos de câmara úmida e com os iniciadores MoT3 e Pot2 por PCR. Houve amplificações de DNA com o primer MoT3 (específico para PoT) em 13 (19,69%) das amostras, todas provenientes da Urochloa. O resultado de que Urochloa hospeda PoT em uma taxa relativamente alta levanta preocupações sobre a importância que essas plantas podem ter no ciclo de brusone do trigo como hospedeiro intermediário para o patógeno e / ou fonte de inóculo para a doença. MenosAbstract: In this study Pyricularia spp., P. oryzae and the P. oryzae pathotype Triticum (PoT) were detected and identified in leaf segments of forage and invasive grasses located in or next to wheat fields. In 2018 and 2019, 66 samples of lesion leaf segments of Urochloa and other grasses were collected in Londrina (PR), Patos de Minas (MG), and Uberaba (MG). The detection and/or identification of the pathogens on the samples was conducted using moist chamber procedures and with the primers MoT3 and Pot2 by PCR. There were DNA amplification with the primer MoT3 (specific for PoT) for 13 (19.69%) of the samples, all of them from Urochloa. The finding that Urochloa hosts PoT at a relatively high rate raises concerns about the importance which these plants may have on the wheat blast cycle as an alternative host for the pathogen and/or source of inoculum for the disease. Resumo: Neste estudo Pyricularia spp., P. oryzae e o patótipo Triticum (PoT) de P. oryzae foram detectados e identificados em segmentos foliares de forrageiras e gramíneas invasoras de lavouras de trigo. Em 2018 e 2019, foram coletadas 66 amostras de segmentos foliares lesionados de Urochloa e outras gramíneas em Londrina (PR), Patos de Minas (MG) e Uberaba (MG). A detecção e/ou identificação dos patógenos nas amostras foi realizada por meio de procedimentos de câmara úmida e com os iniciadores MoT3 e Pot2 por PCR. Houve amplificações de DNA com o primer MoT3 (específico para PoT) em 13 (19,69%) das amostras, ... Mostrar Tudo |
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Palavras-Chave: |
Brusone do trigo; Fonte de inóculo; Primer de PCR MoT3; Primer MoT3; Source of inoculum; Wheat blast. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152083/1/Occurrence-of-Pyricularia-oryzae-JMaciel.pdf
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Marc: |
LEADER 02560naa a2200253 a 4500
001 2152083
005 2023-03-24
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/0103-8478cr20210839$2DOI
100 1 $aMACIEL, J. L. N.
245 $aOccurrence of Pyricularia oryzae Triticum in plants of the genus Urochloa in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: In this study Pyricularia spp., P. oryzae and the P. oryzae pathotype Triticum (PoT) were detected and identified in leaf segments of forage and invasive grasses located in or next to wheat fields. In 2018 and 2019, 66 samples of lesion leaf segments of Urochloa and other grasses were collected in Londrina (PR), Patos de Minas (MG), and Uberaba (MG). The detection and/or identification of the pathogens on the samples was conducted using moist chamber procedures and with the primers MoT3 and Pot2 by PCR. There were DNA amplification with the primer MoT3 (specific for PoT) for 13 (19.69%) of the samples, all of them from Urochloa. The finding that Urochloa hosts PoT at a relatively high rate raises concerns about the importance which these plants may have on the wheat blast cycle as an alternative host for the pathogen and/or source of inoculum for the disease. Resumo: Neste estudo Pyricularia spp., P. oryzae e o patótipo Triticum (PoT) de P. oryzae foram detectados e identificados em segmentos foliares de forrageiras e gramíneas invasoras de lavouras de trigo. Em 2018 e 2019, foram coletadas 66 amostras de segmentos foliares lesionados de Urochloa e outras gramíneas em Londrina (PR), Patos de Minas (MG) e Uberaba (MG). A detecção e/ou identificação dos patógenos nas amostras foi realizada por meio de procedimentos de câmara úmida e com os iniciadores MoT3 e Pot2 por PCR. Houve amplificações de DNA com o primer MoT3 (específico para PoT) em 13 (19,69%) das amostras, todas provenientes da Urochloa. O resultado de que Urochloa hospeda PoT em uma taxa relativamente alta levanta preocupações sobre a importância que essas plantas podem ter no ciclo de brusone do trigo como hospedeiro intermediário para o patógeno e / ou fonte de inóculo para a doença.
653 $aBrusone do trigo
653 $aFonte de inóculo
653 $aPrimer de PCR MoT3
653 $aPrimer MoT3
653 $aSource of inoculum
653 $aWheat blast
700 1 $aKOVALESKI, M.
700 1 $aSILVA, A. N. da
700 1 $aBONATO, A. L. V.
700 1 $aCOSTA, I. F. D. da
773 $tCiência Rural$gv. 53, n. 4, 2023.
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