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| Registros recuperados : 107 | |
| 5. |  | DOMICIANO, G. P.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; LAVIOLA, B. G.; ALVES, A. A. Photosynthetic performance of contrasting Jatropha curcas genotypes during the flowering and fruiting stages. Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 1, p. 10-21, 2018.| Biblioteca(s): Embrapa Agroenergia; Embrapa Unidades Centrais. |
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| 10. | | KOBAYASHI, A. K.; RODRIGUEZ, M. A. D.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Generation of Fusarium f. sp. passiflorae strains expressing reporter genes. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 607.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | DITA, M. A.; KOBAYASHI, A. K.; WAALWIJK, C.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Genetic transformation of Fusarium oxysporum f. sp. cubense. Tropical Plant Pathology, v. 34, p. S177, ago. 2009. Suplemento. Ref. 606. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.| Biblioteca(s): Embrapa Meio-Norte. |
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| 15. | | GUIDUCCI, R. do C. N.; MOLINARI, H. B. C.; PACHECO, T. F.; KOBAYASHI, A. K. Avaliação de impacto ex ante da adoção do ativo tecnológico cana flex para produção de etanol de segunda geração. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 58., 2020, Foz do Iguaçu. Cooperativismo, inovação e sustentabilidade para o desenvolvimento rural: anais... Foz do Iguaçu: UNIOESTE, 2020. 58º SOBER. Grupo de Trabalho (GT): 8. Pesquisa, inovação e extensão rural.| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis as a model plant for functional genomic studies in C4 Crops. In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). p. 49-64| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | KOBAYASHI, A. K.; DITA, M. A.; TROCOLI, R. O.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. GFP and RFP transformation of Fusarium guttiforme (sin. Fusarium subglutinans f. sp. annanas). Tropical Plant Pathology, v. 34, p. S156, ago. 2009. Suplemento. Ref. 545. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.| Biblioteca(s): Embrapa Meio-Norte. |
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| 19. | | GURGEL, F. de L.; LAVIOLA, B. G.; SILVA, D. M. da; KOBAYASHI, A. K.; ROSADO, T. B. Phenology and biometry of physic nut in the Brazilian Savanna Fenologia e biometria de pinhão-manso no cerrado. Ciência e Agrotecnologia, Lavras, v. 35, n. 6, p. 1122-1131, nov./dez. 2011.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 107 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Milho e Sorgo. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
15/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUSA, C. A. F. de; PAIVA, D S. de; CASARI, R. A. das C. N.; MOLINARI, H. B. C.; OLIVEIRA, N. G. de; KOBAYASHI, A. K.; MAGALHAES, P. C.; GOMIDE, R. L.; SOUZA JUNIOR, M. T. |
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Afiliação: |
CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; DAYANE SILVA DE PAIVA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; HUGO BRUNO CORREA MOLINARI, CNPAE; NELSON GERALDO DE OLIVEIRA; ADILSON KENJI KOBAYASHI, CNPAE; PAULO CESAR MAGALHAES, CNPMS; REINALDO LUCIO GOMIDE, CNPMS; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
A procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Methods, v. 13, p. 1-17, 2017. |
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DOI: |
10.1186/s13007-017-0209-z |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time. MenosBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes... Mostrar Tudo |
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Palavras-Chave: |
Phenotyping; Water deficit; Zea mays L. |
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Thesaurus NAL: |
abiotic stress; corn; gas exchange; phenomics. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168295/1/Procedure-maize.pdf
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Marc: |
LEADER 02834naa a2200313 a 4500
001 2081609
005 2018-02-15
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1186/s13007-017-0209-z$2DOI
100 1 $aSOUSA, C. A. F. de
245 $aA procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves.$h[electronic resource]
260 $c2017
520 $aBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time.
650 $aabiotic stress
650 $acorn
650 $agas exchange
650 $aphenomics
653 $aPhenotyping
653 $aWater deficit
653 $aZea mays L
700 1 $aPAIVA, D S. de
700 1 $aCASARI, R. A. das C. N.
700 1 $aMOLINARI, H. B. C.
700 1 $aOLIVEIRA, N. G. de
700 1 $aKOBAYASHI, A. K.
700 1 $aMAGALHAES, P. C.
700 1 $aGOMIDE, R. L.
700 1 $aSOUZA JUNIOR, M. T.
773 $tPlant Methods$gv. 13, p. 1-17, 2017.
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Embrapa Milho e Sorgo (CNPMS) |
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