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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
Data corrente: |
05/06/2020 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
SARAH A. D. RODRIGUES, Universidade de Brasília; THAIS P. PONTELO, Universidade Federal de Lavras; NAYARA R. KUSSANO, Universidade de Brasília; TAYNAN S. KAWAMOTO, Universidade Federal de Uberlândia; LIGIANE O. LEME, Universidade Federal do Espírito Santo; FELIPPE M. C. CAIXETA, Universidade de Brasília; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; MAURICIO MACHAIM FRANCO, Cenargen; MARGOT ALVES NUNES DODE, Cenargen. |
Título: |
Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 72, article 106447, 2020. |
ISSN: |
0739-7240 |
DOI: |
https://doi.org/10.1016/j.domaniend.2020.106447 |
Idioma: |
Inglês |
Conteúdo: |
We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. MenosWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the... Mostrar Tudo |
Palavras-Chave: |
E2; Embryonic development; F2a; Maturation; Oócito; Oocyte competence. |
Thesagro: |
Bovinocultura; Cultura In Vitro; Embriogénese; Maturação; Ovulo; Prostaglandina; Reprodução Animal. |
Thesaurus Nal: |
Animal reproduction; Cattle; Embryogenesis; Gene expression; In vitro studies; Oocytes; Prostaglandins. |
Categoria do assunto: |
-- K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219006/1/cpafro-18481.pdf
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Marc: |
LEADER 02942naa a2200481 a 4500 001 2127907 005 2021-09-15 008 2020 bl uuuu u00u1 u #d 022 $a0739-7240 024 7 $ahttps://doi.org/10.1016/j.domaniend.2020.106447$2DOI 100 1 $aRODRIGUES, S. A. D. 245 $aEffects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes.$h[electronic resource] 260 $c2020 520 $aWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. 650 $aAnimal reproduction 650 $aCattle 650 $aEmbryogenesis 650 $aGene expression 650 $aIn vitro studies 650 $aOocytes 650 $aProstaglandins 650 $aBovinocultura 650 $aCultura In Vitro 650 $aEmbriogénese 650 $aMaturação 650 $aOvulo 650 $aProstaglandina 650 $aReprodução Animal 653 $aE2 653 $aEmbryonic development 653 $aF2a 653 $aMaturation 653 $aOócito 653 $aOocyte competence 700 1 $aPONTELO, T. P. 700 1 $aKUSSANO, N. R. 700 1 $aKAWAMOTO, T. S. 700 1 $aLEME, L. O. 700 1 $aCAIXETA, F. M. C. 700 1 $aPFEIFER, L. F. M. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tDomestic Animal Endocrinology$gv. 72, article 106447, 2020.
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Embrapa Rondônia (CPAF-RO) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
06/04/2004 |
Data da última atualização: |
27/07/2007 |
Autoria: |
KLINGELFUSS, L. H.; YORINORI, J. T.; DESTRO, D. |
Título: |
Identification methods for resistance in soybean to Fusarium solani f. sp. glycines, under greenhouse conditions. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
Páginas: |
p. 79. |
Série: |
(Embrapa Soja. Documentos, 228). |
Idioma: |
Inglês |
Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
Conteúdo: |
Sudden death syndrome (SDS) caused by Fusarium solani f. sp. glycines has became one of the most important diseases in soybean due to significant yield losses and control limitations. Control has been possible by means of resistant genes. This work aimed to identify efficient methods of inoculation to assess the genetic resistance of genotypes of soybean to SDS. Two experiments were arranged in a randomized block design (RBD). The former consisted of six treatments and 12 replicates and the latter, six treatments and 16 replicates. Each plot was constituted of a plant pot containing one plant of the cultivar FT Estrela, witch is highly susceptible to SDS and another plant of the cultivar Conquista, which is moderately resistant. The plants were assessed in relation to incidence and severity of SDS, on every three days, for fifteen days, starting from the fifteenth day of emergence. From the data obtained, the area under the disease progress curve (AUDPC) was estimated for the treatments. The inoculations with grains of sorghum and corn were the most effective in causing the appearance of the disease, in the first experiment. Inoculations with corn kernels were the most effective method in causing the disease, in the second experiment. However, only the inoculation with corn kernels permitted a separate reaction of genotypes in relation to SDS resistance, according to Tukey test (P<0,05), in both experiments. Thus, the inoculation with corn kernels can be recommended to reaction assessments of soybean genotypes to SDS under greenhouse conditions. MenosSudden death syndrome (SDS) caused by Fusarium solani f. sp. glycines has became one of the most important diseases in soybean due to significant yield losses and control limitations. Control has been possible by means of resistant genes. This work aimed to identify efficient methods of inoculation to assess the genetic resistance of genotypes of soybean to SDS. Two experiments were arranged in a randomized block design (RBD). The former consisted of six treatments and 12 replicates and the latter, six treatments and 16 replicates. Each plot was constituted of a plant pot containing one plant of the cultivar FT Estrela, witch is highly susceptible to SDS and another plant of the cultivar Conquista, which is moderately resistant. The plants were assessed in relation to incidence and severity of SDS, on every three days, for fifteen days, starting from the fifteenth day of emergence. From the data obtained, the area under the disease progress curve (AUDPC) was estimated for the treatments. The inoculations with grains of sorghum and corn were the most effective in causing the appearance of the disease, in the first experiment. Inoculations with corn kernels were the most effective method in causing the disease, in the second experiment. However, only the inoculation with corn kernels permitted a separate reaction of genotypes in relation to SDS resistance, according to Tukey test (P<0,05), in both experiments. Thus, the inoculation with corn kernels can be recommended to react... Mostrar Tudo |
Categoria do assunto: |
-- |
Marc: |
LEADER 02479naa a2200181 a 4500 001 1466755 005 2007-07-27 008 2004 bl uuuu u00u1 u #d 100 1 $aKLINGELFUSS, L. H. 245 $aIdentification methods for resistance in soybean to Fusarium solani f. sp. glycines, under greenhouse conditions. 260 $c2004 300 $ap. 79. 490 $a(Embrapa Soja. Documentos, 228). 500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. 520 $aSudden death syndrome (SDS) caused by Fusarium solani f. sp. glycines has became one of the most important diseases in soybean due to significant yield losses and control limitations. Control has been possible by means of resistant genes. This work aimed to identify efficient methods of inoculation to assess the genetic resistance of genotypes of soybean to SDS. Two experiments were arranged in a randomized block design (RBD). The former consisted of six treatments and 12 replicates and the latter, six treatments and 16 replicates. Each plot was constituted of a plant pot containing one plant of the cultivar FT Estrela, witch is highly susceptible to SDS and another plant of the cultivar Conquista, which is moderately resistant. The plants were assessed in relation to incidence and severity of SDS, on every three days, for fifteen days, starting from the fifteenth day of emergence. From the data obtained, the area under the disease progress curve (AUDPC) was estimated for the treatments. The inoculations with grains of sorghum and corn were the most effective in causing the appearance of the disease, in the first experiment. Inoculations with corn kernels were the most effective method in causing the disease, in the second experiment. However, only the inoculation with corn kernels permitted a separate reaction of genotypes in relation to SDS resistance, according to Tukey test (P<0,05), in both experiments. Thus, the inoculation with corn kernels can be recommended to reaction assessments of soybean genotypes to SDS under greenhouse conditions. 700 1 $aYORINORI, J. T. 700 1 $aDESTRO, D. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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