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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
30/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PILLAI, V. V.; SIQUEIRA, L. G. B.; DAS, M.; KEI, T. G.; TU, L. N.; HERREN, A. W.; PHINNEY, B. S.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. |
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Afiliação: |
VIJU VIJAYAN PILLAI, Cornell University, Ithaca, NY; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MOUBANI DAS, Cornell University, Ithaca, NY; TIFFANY G. KEI, Cornell University, Ithaca, NY; LAN N. TU, Cornell University, Ithaca, NY; ANTHONY W. HERREN, University of California; BRETT S. PHINNEY, University of California; SOON HON CHEONG, Cornell University, Ithaca, NY; PETER J. HANSEN, University of Florida, Gainesville, FL; VIMAL SELVARAJ, Cornell University, Ithaca, NY. |
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Título: |
Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biology Open, v. 8, n. 5, article bio037937, 2019. |
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DOI: |
https://doi.org/10.1242/bio.037937 |
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Idioma: |
Inglês |
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Conteúdo: |
Trophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation. MenosTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understandi... Mostrar Tudo |
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Thesagro: |
Placenta. |
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Thesaurus Nal: |
Blastocyst; Embryo implantation; Pregnancy; Stem cells; Trophoblast. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207915/1/BiolOpen-LGustavoSiq-Physiological-bio037937.full.pdf
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Marc: |
LEADER 02435naa a2200313 a 4500
001 2117829
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008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1242/bio.037937$2DOI
100 1 $aPILLAI, V. V.
245 $aPhysiological profile of undifferentiated bovine blastocyst-derived trophoblasts.$h[electronic resource]
260 $c2019
520 $aTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation.
650 $aBlastocyst
650 $aEmbryo implantation
650 $aPregnancy
650 $aStem cells
650 $aTrophoblast
650 $aPlacenta
700 1 $aSIQUEIRA, L. G. B.
700 1 $aDAS, M.
700 1 $aKEI, T. G.
700 1 $aTU, L. N.
700 1 $aHERREN, A. W.
700 1 $aPHINNEY, B. S.
700 1 $aCHEONG, S. H.
700 1 $aHANSEN, P. J.
700 1 $aSELVARAJ, V.
773 $tBiology Open$gv. 8, n. 5, article bio037937, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
18/03/2011 |
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Data da última atualização: |
19/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BENKO-ISEPPON, A. M.; KIDO, E. A.; PANDOLFI, V.; BARBOSA, P. K. A; BELARMINO, L. C.; MONTE, S. J. H. do; BRANDÃO, R. M. S. de; ARAUJO, A. de S.; CASTRO, J. A. F. de; SOARES-CAVALCANTI, N. da M.; SILVA, A. R. da; CALSA JUNIOR, T.; ROCHA, M. de M.; WINTER, P.; KAHL, G.; ROTTER, B.; HORRES, R.; MOLINA, C.; JUNGMANN, R.; AMORIM, L. L. B.; ONOFRE, A. V. C.; FERREIRA-NETO, J. R. C.; GRANJEIRO, T. B.; LIMA, A. S.; LOBO, M. D. P.; HOULLOU-KIDO, L. M.; CARVALHO, R. de; WANDERLEY-NOGUEIRA, A. C.; BARROS, P. dos S.; VIEIRA-MELLO, G. S.; BRASILEIRO-VIDAL, A. C.; BORTOLETI, K. C. de A.; PEDROSA-HARAND, A.; ANDRADE, P. P. de; ANDRADE, G. P. de; PIO-RIBEIRO, G.; SITTOLIN, I. M.; FREIRE FILHO, F. R. |
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Afiliação: |
ANA M. BENKO-ISEPPON, UFPE; EDERSON A. KIDO, UFPE; VALESCA PANDOLFI, UFPE; PEDRANNE K. A. BARBOSA, UFPE; LUIZ C. BELARMINO, UFPE; SEMIRAMIS J. H. DO MONTE, UFPI; RAFAEL M. S. DE S. BRANDÃO, UFPI; ANAREGINA DE S. ARAUJO, UFPI; JOSE A. F. DE CASTRO, UFPI; NINA DA M. SOARES-CAVALCANTI, UFPE; ADRIANO R. DA SILVA, UFPE; TERCILIO CALSA JUNIOR, UFPE; MAURISRAEL DE MOURA ROCHA, CPAMN; PETER WINTER, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; GUNTER KAHL, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; BJORN ROTTER, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; RALF HORRES, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; CARLOS MOLINA, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; RUTH JUNGMANN, JOHANN WOLÍGANG GOETHE UNIVERSITAT GENXPRO, FRANKFURT AM MAIN, GERMARY; LIDIANE L. B. AMORIM, UFPE; ALBERTO V. C. ONOFRE, UFPE; JOSE R. C. FERREIRA-NETO, UFPE; THALLES B. GRANJEIRO, UFC; ADRIANA S. LIMA, UFC; MARINA D. P. LOBO, UFC; LAUREEN M. HOULLOU-KIDO, CETENE; REGINALDO DE CARVALHO, UFPE; ANA C. WANDERLEY-NOGUEIRA, UFPE; PETRA DOS S. BARROS, UFPE; GABRIELA S. VIEIRA-MELLO, UFPE; ANA C. BRASILEIRO-VIDAL, UFPE; KYRIA C. DE A. BORTOLETI, UFPE; ANDREA PEDROSA-HARAND, UFPE; PAULO P. DE ANDRADE, UFPE; GENIRA P. DE ANDRADE, UFPE; GILVAN PIO-RIBEIRO, UFRPE; ILZA MARIA SITTOLIN, CPAMN; FRANCISCO RODRIGUES FREIRE FILHO, CPAMN. |
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Título: |
Brazilian cowpea transcriptome project: over 20 million expressed sequence tags to understand salinity and virus resistance. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010. Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. |
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Páginas: |
p. 97-98. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Feijão-caupi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30057/1/Brazilian0001.pdf
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Marc: |
LEADER 01714nam a2200565 a 4500
001 1881607
005 2023-12-19
008 2010 bl uuuu u00u1 u #d
100 1 $aBENKO-ISEPPON, A. M.
245 $aBrazilian cowpea transcriptome project$bover 20 million expressed sequence tags to understand salinity and virus resistance.
260 $aIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010. Fortaleza. Programa e resumos. Brasília, DF: SBBiotec$c2010
300 $ap. 97-98.
653 $aFeijão-caupi
700 1 $aKIDO, E. A.
700 1 $aPANDOLFI, V.
700 1 $aBARBOSA, P. K. A
700 1 $aBELARMINO, L. C.
700 1 $aMONTE, S. J. H. do
700 1 $aBRANDÃO, R. M. S. de
700 1 $aARAUJO, A. de S.
700 1 $aCASTRO, J. A. F. de
700 1 $aSOARES-CAVALCANTI, N. da M.
700 1 $aSILVA, A. R. da
700 1 $aCALSA JUNIOR, T.
700 1 $aROCHA, M. de M.
700 1 $aWINTER, P.
700 1 $aKAHL, G.
700 1 $aROTTER, B.
700 1 $aHORRES, R.
700 1 $aMOLINA, C.
700 1 $aJUNGMANN, R.
700 1 $aAMORIM, L. L. B.
700 1 $aONOFRE, A. V. C.
700 1 $aFERREIRA-NETO, J. R. C.
700 1 $aGRANJEIRO, T. B.
700 1 $aLIMA, A. S.
700 1 $aLOBO, M. D. P.
700 1 $aHOULLOU-KIDO, L. M.
700 1 $aCARVALHO, R. de
700 1 $aWANDERLEY-NOGUEIRA, A. C.
700 1 $aBARROS, P. dos S.
700 1 $aVIEIRA-MELLO, G. S.
700 1 $aBRASILEIRO-VIDAL, A. C.
700 1 $aBORTOLETI, K. C. de A.
700 1 $aPEDROSA-HARAND, A.
700 1 $aANDRADE, P. P. de
700 1 $aANDRADE, G. P. de
700 1 $aPIO-RIBEIRO, G.
700 1 $aSITTOLIN, I. M.
700 1 $aFREIRE FILHO, F. R.
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Embrapa Meio-Norte (CPAMN) |
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