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| Registros recuperados : 229 | |
| 161. |  | BRANDALISE, L.; SIMÃO, G. M. R.; SATO, J. P. H.; PIGOZZO, R.; NAGAE, R. Y.; CLAVIJO, M. J.; KICH, J. D.; TAKEUTI, K. L.; DEZEN, D. Aclimatação de leitoas de reposição negativas para Mycoplasma hyopneumoniae expostas naturalmente ao agente. In: SIMPÓSIO INTERNACIONAL DE SUINOCULTURA, 13., 2021, Online. Anais... Porto Alegre: UFRGS, 2021. SINSUI.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 162. | | PISSETTI, C.; KICH, J. D.; ALLEN, H. K.; NAVARRETE, C.; COSTA, E. de F.; MORÉS, N.; CARDOSO, M. R. de I. Antimicrobial resistance in commensal Escherichia coli and Enterococcus spp. isolated from pigs subjected to different antimicrobial administration protocols. Research in Veterinary Science, v. 137, p. 174?185, 2021.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 163. | | TUTIDA, Y. H.; SIQUEIRA, H. A.; MONTES, J. H.; HEBBEL, C.; NARDELLI, G.; PERIPOLLI, V.; MOREIRA, F.; GÜTHS, M. F.; IRGANG, R.; KICH, J. D.; BIANCHI, I. Alternativas ao uso de antibióticos via ração na produção de Suínos. In: SIMPÓSIO INTERNACIONAL DE SUINOCULTURA, 13., 2021, Online. Anais... Porto Alegre: UFRGS, 2021. SINSUI.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 164. | | PELLEGRINI, D. da C. P.; PAIM, D. dos S.; LIMA, G. J. M. M. de; KICH, J. D.; COLDEBELLA, A.; CARDOSO, M. R. de I. Inspeção de boas práticas de fabricação e enumeração de coliformes totais em fábricas de ração para suínos. Semina: Ciências Agrárias, Londrina, v. 34, n. 6, supl. 2, p. 3767-3776, 2013.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 165. | | KICH, J. D.; SOUZA, A. I. A.; MONTES, J.; MENEGUZZI, M.; COSTA, E. de F.; COLDEBELLA, A.; CORBELLINI, L. G.; CARDOSO, M. R. de I. Investigation of Listeria monocytogenes, Salmonella enterica and Yersinia enterocolitica in pig carcasses in southern Brazil. Pesquisa Veterinária Brasileira, v. 40, n. 10, p. 781-790, 2020.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 166. | | SILVA, L. E. da; DIAS, V.; FERRONATTO, A.; GUERRA, P.; BERNO, L.; TRICHES, N.; KICH, J. D.; CORBELLINI, L. G.; CARDOSO, M. Longitudinal dissemination of salmonella enterica clonal groups through the slaughter process of salmonella-positive pig batches. Journal of Food Protection, v. 75, n. 9, p. 1580?1588, 2012. Projeto: 04.10.06.001.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 167. | | TUTIDA, Y. H.; BORSTNEZ, K. K.; MONTES, J.; GÜTHS, M. F.; IRGANG, R.; MOREIRA, F.; PERIPOLLI, V.; MORÉS, N.; BIANCHI, I.; KICH, J. D. Estratégias para reduzir o uso de antibióticos na produção de suínos. In: SIMPÓSIO INTERNACIONAL DE SUINOCULTURA, 12, 2019, Porto Alegre. Avanços em sanidade, produção e reprodução de suínos IV: anais. Porto Alegre: UFRGS, 2019, p. 282-283.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 168. | | AMARAL, A. L. do; SILVA, V. S.; MORES, N.; KRAMER, B.; PRETTO, A. N.; RAMENZONI, M. L. F.; KICH, J. D.; COLDEBELLA, A. Fatores importantes para evitar a contaminação residual das instalações de creche de suínos por Salmonella ao alojamento. Concórdia: Embrapa Suínos e Aves, 2006. 3 p. (Embrapa Suínos e Aves. Comunicado Técnico, 445)| Biblioteca(s): Embrapa Suínos e Aves. |
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| 169. | | SILVA, V. S.; KRAMER, B.; AMARAL, A. L. do; MORÉS, N.; KICH, J. D.; BORDIN, L. C.; PRETTO, N. A.; COLDEBELLA, A. Fatores de risco associados à contaminação residual por enterobactérias em instalações de creche de suínos após vazio entre lotes. In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 13., 2007, Florianópolis. Anais... Concórdia: Embrapa Suínos e Aves, 2007. 1 CD-ROM. Projeto n. 03.04.35.100-02.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 170. | | KICH, J. D.; MORES, N.; VIDAL, C. E. S.; PIFFER, I. A.; BARIONI JUNIOR, W.; AMARAL, A. L. do; RAMMINGER, L.; CARDOSO, M. de I. Fatores de risco associados com a prevalência sorológica de salmonela em granjas comerciais de suínos do sul do Brasil. Concórdia: EMBRAPA-CNPSA, 2002 5 (EMBRAPA-CNPSA. Comunicado técnico, 309)| Biblioteca(s): Embrapa Suínos e Aves. |
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| 171. | | KICH, J. D.; MORES, N.; VIDAL, C. E. S.; PIFFER, I. A.; BARIONI JÚNIOR, W.; AMARAL, A. L. do; RAMMINGER, L.; CARDOSO, M. de I. Fatores de risco associaods com a prevalência sorológica de salmonela em granjas comerciais de suínos do Sul do Brasil. Concórdia: EMBRAPA-CNPSA, 2002 5 (EMBRAPA-CNPSA. Comunicado técnico, 309)| Biblioteca(s): Embrapa Suínos e Aves. |
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| 172. | | CAMARGO, J. de; MARQUES, M. G.; FACCIN, J. C. V.; JORGE NETO, P. N.; KICH, J. D.; SCARIOT, C. A.; ZANELLA, R. The feasibility of a swine semen storage system produced with bacteriostatic molecules to control bacterial proliferation. In: INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION, 9., 2022, Bento Gonçalves. Abstracts...Belo Horizonte: CBRA, 2022. Animal Reproduction, v. 19, n. 4, 2022.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 173. | | SATO, J. P. H.; PIGOZZO, R.; BRANDALISE, L.; LIMA, G. F. R.; CERIZOLLI, R.; KUCHIISHI, S. S.; KICH, J. D.; SIMÃO, G. M. R. Evaluation of the thermo-assisted drying and decontamination system and ozone gas for sanitation of livestock transport vehicles. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 25., 2018, Chongqing. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 25., 2018, Chongqing. Healthy pig , safe pork: proceedings: poster presentations. Chongqing: IPVS, 2018. p. 237. v. 1.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 174. | | COSTA, E. de F.; KICH, J. D.; MIELE, M.; MORÉS, N.; AMARAL, A. L. do; COLDEBELLA, A.; CARDOSO, M. R. de I.; CORBELLINI, L. G. Evaluation of two strategies for reducing the spread of Salmonella in commercial swine herds during the finishing phase and their incremental cost-effectiveness ratios. Semina: Ciências Agrárias, Londrina, v. 41, n. 2, p. 505-516.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 175. | | PALHARES, J. C. P.; GUIDONI, A. L.; KICH, J. D.; STEINMETZ, R. L. R.; MULINARI, M. R.; KLEIN, J. B.; COLDEBELLA, L.; PLIESKE, G.; SIGUA, G. C. Evaluation of adjustment environmental contract to pig production in Pinhal River sub-basin In: CENTURY WATERSHED TECHNOLOGY: IMPROVING WATER QUALITY AND ENVIRONMENT CONFERENCE, 21., 2010, Costa Rica. Proceedings... Michigan: ASABE, 2010. Projeto/Plano de Ação: 16.00.30004-00| Biblioteca(s): Embrapa Suínos e Aves. |
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| 176. | | DALLA COSTA, O. A.; LUDKE, J. V.; COLDEBELLA, A.; KICH, J. D.; COSTA, M. J. R. P da; FAUCITANO, L.; PELOSO, J. V.; ROZA, D. D. Efeito do manejo pré-abate sobre alguns parâmetros fisiológicos em fêmeas suínas pesadas Ciência Rural, v.39, n.3, p.852-858, 2009. Disponível em: . Acesso em: 10 fev. 2010 Projeto/Plano de Ação: 02.06.10.100-01| Biblioteca(s): Embrapa Suínos e Aves. |
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| 177. | | ARAUJO, F. R.; BIER, D.; VERBISCK, N. V.; RAMOS, C. L. do N.; RODRIGUES, D. dos P.; VALSONI, L. M.; KICH, J. D.; DUARTE, S. C. Detecção de Salmonella spp. a partir de carcaças de bovinos obtidas durante o processamento em abatedouros-frigoríficos em Mato Grosso do Sul: resultados preliminares Campo Grande, MS: Embrapa Gado de Corte, 2015. 6 p. (Embrapa Gado de Corte. Comunicado técnico, 131).| Biblioteca(s): Embrapa Gado de Corte; Embrapa Suínos e Aves. |
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| 178. | | CANTAO, M. E.; OLIVEIRA FILHO, J. X. de; KICH, J. D.; KLEIN, C. S.; ZANELLA, R.; MORES, M. A. Z.; REBELATTO, R.; MORES, N. Draft genome sequence of Pasteurella multocida strain 11246. In: INTERNATIONAL CONFERENCE OF THE AB3C; BRAZILIAN SYMPOSIUM ON BIOINFORMATICS, 2013, Recife. Abstract book... Recife: Brazilian Association for Bioinformatics and Computer Biology; Braziian Computer Society, 2013. Não paginado. X-meeting BSB 2013. Pôster.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 179. | | SERIBELLI, A. A.; FRAZÃO, M. R.; GONZALES, J. C.; CAO, G.; LEON, M. S.; KICH, J. D.; ALLARD, M. W.; FALCÃO, J. P. Draft Genome Sequences of 20 Salmonella enterica subsp. enterica Serovar Typhimurium Strains Isolated from Swine in Santa Catarina, Brazil. Genome Announcements, v. 6, issue 16, e00232-18, 2018.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 180. | | DALLA COSTA, O. A.; LUDKE, J. V.; COSTA, M. J. R. P. da; COLDEBELLA, A.; FAUCITANO, L.; PELOSO, J. V.; TRIQUES, N. J.; KICH, J. D. Determinação do ritmo ultradiano do cortisol na saliva de fêmeas suínas em peso de abate. Concórdia: Embrapa Suínos e Aves, 2007. 3 p. (Embrapa Suínos e Aves. Comunicado Técnico, 460). Projeto n. 02.06.10.100-02.| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 229 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
31/10/2019 |
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Data da última atualização: |
31/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
PAIM, D. S.; PISSETTI, C.; VIEIRA, T. R.; WERLANG, G. O.; COSTA, E. de F.; KICH, J. D.; CARDOSO, M. R. de I. |
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Afiliação: |
DANIEL SANTOS PAIM, UFRGS; CAROLINE PISSETTI, UFRGS; TATIANA REGINA VIEIRA, UFRGS; GABRIELA OROSCO WERLANG, UFRGS; EDUARDO DE FREITAS COSTA, UFRGS; JALUSA DEON KICH, CNPSA; MARISA RIBEIRO DE ITAPEMA CARDOSO, UFRGS. |
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Título: |
Enumeration, antimicrobial resistance and typing of salmonella enterica: profile of strains carried in the intestinal contents of pigs at slaughter in southern Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 47, ed. 1636, 2019. |
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DOI: |
10.22456/1679-9216.89668 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: Background: Despite a strong association between Salmonella isolation and slaughter hygiene, as measured by the Enterobacteriaceae levels on pre-chill carcass surfaces, a high variation in this association was observed between sampling days within the same slaughterhouse. It was hypothesised that in a scenario of high exposure on the farm, batches with a high prevalence of carrier pigs shedding a high number of Salmonella may enhance the risk of contamination on some slaughter days. Thus, the aim of this study was to assess the profile of Salmonella carried in the intestinal contents of slaughter pigs. Materials, Methods & Results: Ten pig batches slaughtered in a slaughterhouse were investigated for the presence of Salmonella. From each pig, the following samples were taken: i. blood collected at bleeding; ii. sponges rubbed on the carcass surface after bleeding and before chilling; iii. fragment of the ileocecal region of the intestine. Serum samples were subjected to a ELISA-Typhimurium test. Sponges were investigated for the presence of Salmonella and total aerobic mesophilic (TAM) and terobacteriaceae (EC) bacterial counts. Salmonella was enumerated in the intestinal contents. Selected Salmonella strains were subjected to an antimicrobial resistance disk diffusion test, macro-restriction with Xba-I (PFGE) and whole genome sequencing (WGS). From the 50 sampled pigs, 96% were positive in the ELISA-Typhimurium test and 64% were Salmonella-positive in the intestinal contents. The amount of Salmonella in the intestinal content samples was highly variable, and the mean log of fitted distributions of Salmonella in the batch ranged from -2.97 to 2.25 cfu.g-1. The slaughter process achieved a logarithmic reduction, ranging from 0.64 to 2.35 log cfu.cm-2 for TAM and from 0.55 to 2.57 log cfu.cm-2 for EC. Salmonella was isolated from 16% of the carcasses after bleeding; this frequency decreased to 8% at the pre-chill step. All positive pre-chill carcasses originated from pigs carrying Salmonella in the intestinal content and from batches with a high number of carrier pigs. Salmonella Typhimurium and its monophasic variant were the most frequent in the intestinal contents and carcasses. Resistance was detected against ampicillin (42.5%), tetracycline (42.5%), sulfonamide (40%), gentamicin (25%) and ciprofloxacin (12.5%). Regarding colistin, 85% of the tested strains were classified as non-susceptible. The monophasic variant S. Typhimurium strains subjected to PFGE and WGS presented different profiles; several antimicrobial resistance genes were identified and all belonged to ST-19. Discussion: In this study, almost all sampled pigs entering the slaughter line had been exposed to Salmonella on the farm and a high number were carrying Salmonella in their guts. While the three batches with Salmonella-positive carcasses at the pre-chill step presented TAM media that was not significantly different from the other batches, there was a higher number of positive pigs carrying Salmonella in their intestinal contents. Moreover, the batch with the highest number of positive carcasses also presented the highest Salmonella mean count in their intestinal contents. The profile of Salmonella carried in the intestinal content of slaughter pigs proved to be highly variable in terms of the frequency, number of bacteria, serovars, antimicrobial resistance, and genotypes. Results indicate that the day-to-day variability in the prevalence and number of Salmonella in the intestinal contents of slaughter batches is likely to influence the frequency of contaminated pre-chill carcasses. Salmonella Typhimurium isolated from the intestinal contents of slaughter pigs may belong to genotypes involved in human disease and may carry several antimicrobial resistance genes. These aspects should be taken into account when planning Salmonella control in swine. MenosABSTRACT: Background: Despite a strong association between Salmonella isolation and slaughter hygiene, as measured by the Enterobacteriaceae levels on pre-chill carcass surfaces, a high variation in this association was observed between sampling days within the same slaughterhouse. It was hypothesised that in a scenario of high exposure on the farm, batches with a high prevalence of carrier pigs shedding a high number of Salmonella may enhance the risk of contamination on some slaughter days. Thus, the aim of this study was to assess the profile of Salmonella carried in the intestinal contents of slaughter pigs. Materials, Methods & Results: Ten pig batches slaughtered in a slaughterhouse were investigated for the presence of Salmonella. From each pig, the following samples were taken: i. blood collected at bleeding; ii. sponges rubbed on the carcass surface after bleeding and before chilling; iii. fragment of the ileocecal region of the intestine. Serum samples were subjected to a ELISA-Typhimurium test. Sponges were investigated for the presence of Salmonella and total aerobic mesophilic (TAM) and terobacteriaceae (EC) bacterial counts. Salmonella was enumerated in the intestinal contents. Selected Salmonella strains were subjected to an antimicrobial resistance disk diffusion test, macro-restriction with Xba-I (PFGE) and whole genome sequencing (WGS). From the 50 sampled pigs, 96% were positive in the ELISA-Typhimurium test and 64% were Salmonella-positive in the intestin... Mostrar Tudo |
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Palavras-Chave: |
Enterobacteria; Faecal excretion; MCR-1 gene; Salmonella Typhimurium monophasic variant; Total aerobic mesophilic bacteria; Whole genome sequencing. |
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Thesagro: |
Salmonella; Suíno. |
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Thesaurus NAL: |
Salmonella enterica; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203969/1/final9102.pdf
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Marc: |
LEADER 04942naa a2200325 a 4500
001 2113682
005 2019-10-31
008 2019 bl uuuu u00u1 u #d
024 7 $a10.22456/1679-9216.89668$2DOI
100 1 $aPAIM, D. S.
245 $aEnumeration, antimicrobial resistance and typing of salmonella enterica$bprofile of strains carried in the intestinal contents of pigs at slaughter in southern Brazil.$h[electronic resource]
260 $c2019
520 $aABSTRACT: Background: Despite a strong association between Salmonella isolation and slaughter hygiene, as measured by the Enterobacteriaceae levels on pre-chill carcass surfaces, a high variation in this association was observed between sampling days within the same slaughterhouse. It was hypothesised that in a scenario of high exposure on the farm, batches with a high prevalence of carrier pigs shedding a high number of Salmonella may enhance the risk of contamination on some slaughter days. Thus, the aim of this study was to assess the profile of Salmonella carried in the intestinal contents of slaughter pigs. Materials, Methods & Results: Ten pig batches slaughtered in a slaughterhouse were investigated for the presence of Salmonella. From each pig, the following samples were taken: i. blood collected at bleeding; ii. sponges rubbed on the carcass surface after bleeding and before chilling; iii. fragment of the ileocecal region of the intestine. Serum samples were subjected to a ELISA-Typhimurium test. Sponges were investigated for the presence of Salmonella and total aerobic mesophilic (TAM) and terobacteriaceae (EC) bacterial counts. Salmonella was enumerated in the intestinal contents. Selected Salmonella strains were subjected to an antimicrobial resistance disk diffusion test, macro-restriction with Xba-I (PFGE) and whole genome sequencing (WGS). From the 50 sampled pigs, 96% were positive in the ELISA-Typhimurium test and 64% were Salmonella-positive in the intestinal contents. The amount of Salmonella in the intestinal content samples was highly variable, and the mean log of fitted distributions of Salmonella in the batch ranged from -2.97 to 2.25 cfu.g-1. The slaughter process achieved a logarithmic reduction, ranging from 0.64 to 2.35 log cfu.cm-2 for TAM and from 0.55 to 2.57 log cfu.cm-2 for EC. Salmonella was isolated from 16% of the carcasses after bleeding; this frequency decreased to 8% at the pre-chill step. All positive pre-chill carcasses originated from pigs carrying Salmonella in the intestinal content and from batches with a high number of carrier pigs. Salmonella Typhimurium and its monophasic variant were the most frequent in the intestinal contents and carcasses. Resistance was detected against ampicillin (42.5%), tetracycline (42.5%), sulfonamide (40%), gentamicin (25%) and ciprofloxacin (12.5%). Regarding colistin, 85% of the tested strains were classified as non-susceptible. The monophasic variant S. Typhimurium strains subjected to PFGE and WGS presented different profiles; several antimicrobial resistance genes were identified and all belonged to ST-19. Discussion: In this study, almost all sampled pigs entering the slaughter line had been exposed to Salmonella on the farm and a high number were carrying Salmonella in their guts. While the three batches with Salmonella-positive carcasses at the pre-chill step presented TAM media that was not significantly different from the other batches, there was a higher number of positive pigs carrying Salmonella in their intestinal contents. Moreover, the batch with the highest number of positive carcasses also presented the highest Salmonella mean count in their intestinal contents. The profile of Salmonella carried in the intestinal content of slaughter pigs proved to be highly variable in terms of the frequency, number of bacteria, serovars, antimicrobial resistance, and genotypes. Results indicate that the day-to-day variability in the prevalence and number of Salmonella in the intestinal contents of slaughter batches is likely to influence the frequency of contaminated pre-chill carcasses. Salmonella Typhimurium isolated from the intestinal contents of slaughter pigs may belong to genotypes involved in human disease and may carry several antimicrobial resistance genes. These aspects should be taken into account when planning Salmonella control in swine.
650 $aSalmonella enterica
650 $aSwine
650 $aSalmonella
650 $aSuíno
653 $aEnterobacteria
653 $aFaecal excretion
653 $aMCR-1 gene
653 $aSalmonella Typhimurium monophasic variant
653 $aTotal aerobic mesophilic bacteria
653 $aWhole genome sequencing
700 1 $aPISSETTI, C.
700 1 $aVIEIRA, T. R.
700 1 $aWERLANG, G. O.
700 1 $aCOSTA, E. de F.
700 1 $aKICH, J. D.
700 1 $aCARDOSO, M. R. de I.
773 $tActa Scientiae Veterinariae$gv. 47, ed. 1636, 2019.
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Embrapa Suínos e Aves (CNPSA) |
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