Data/hora: 17/06/2024 19:11:39

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 22/11/2019
Data da última atualização: | 10/03/2020
Autoria: | VIANA, C.; SERENO, M. J.; BERSOT, L. dos S.; KICH, J. D.; NERO, L. A.
Afiliação: | CIBELI VIANA, UFV; MALLU JAGNOW SERENO, UFPR; LUCIANO DOS SANTOS BERSOT, UFPR; JALUSA DEON KICH, CNPSA; LUIS AUGUSTO NERO, UFV.
Título: | Comparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain.
Ano de publicação: | 2020
Fonte/Imprenta: | Foodborne Pathogens and Disease, v. 17, n. 3, p. 194-201, 2020.
DOI: | 10.1089/fpd.2019.2712
Idioma: | Inglês
Conteúdo: | Abstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n&#8201;=&#8201;100); environment (barn floor, n&#8201;=&#8201;10, and lairage floor, n&#8201;=&#8201;10); meat juice (n&#8201;=&#8201;100, obtained after defrosting of diaphragm); tonsils (n&#8201;=&#8201;100); mesenteric lymph nodes (MLNs) (n&#8201;=&#8201;100); and carcasses after bleeding (n&#8201;=&#8201;100), after singeing (n&#8201;=&#8201;100), after evisceration (n&#8201;=&#8201;100), and after final rinsing (n&#8201;=&#8201;100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r&#8201;=&#8201;0.93, p&#8201;<&#8201;0.001), and a substantial agreement for Salmonella detection (K&#8201;=&#8201;0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K&#8201;=&#8201;0.49 and 0.50, respectively) and tonsils (K&#8201;=&#8201;0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering.
Thesagro: | Bacteriologia; Cadeia Produtiva; Carne; Elisa; Enzima; Salmonella; Suíno; Técnica Imunoenzimática.
Thesaurus Nal: | Bacteriology; Disease surveillance; Enzyme-linked immunosorbent assay; Isolation techniques; Pork.
Categoria do assunto: | --
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (21735-1) - UPC - AP - DD -  -  -  - DIS