Data/hora: 18/06/2024 00:52:56

Biblioteca(s): | Embrapa Suínos e Aves; Embrapa Unidades Centrais.
Data corrente: | 02/02/2018
Data da última atualização: | 20/06/2018
Autoria: | PISSETTI, C.; WERLANG, G. O.; KICH, J. D.; CARDOSO, M.
Afiliação: | Caroline Pissetti, Departamento de Medicina Veterinária Preventiva/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Gabriela Orosco Werlang, Departamento de Medicina Veterinária Preventiva/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; JALUSA DEON KICH, CNPSA; Marisa Cardoso, Departamento de Medicina Veterinária Preventiva/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS.
Título: | Genotyping and antimicrobial resistance in Escherichia coli from pig carcasses.
Ano de publicação: | 2017
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 11, p. 1253-1260, novembro. 2017.
Idioma: | Inglês
Notas: | Título em português: Genotipagem e resistência antimicrobiana de Escherichia coli em carcaças suínas.
Conteúdo: | The increasing antimicrobial resistance observed worldwide in bacteria isolated from human and animals is a matter of extreme concern and has led to the monitoring of antimicrobial resistance in pathogenic and commensal bacteria. The aim of this study was to evaluate the antimicrobial resistance profile of Escherichia coli isolated from pig carcasses and to assess the occurrence of relevant resistance genes. A total of 319 E. coli isolates were tested for antimicrobial susceptibility against different antimicrobial agents. Moreover, the presence of extended-spectrum ?-lactamase (ESBL) and inducible ampC-?-lactamase producers was investigated. Eighteen multi-resistant strains were chosen for resistance gene detection and PFGE characterization. The study showed that resistance to antimicrobials is widespread in E. coli isolated from pig carcasses, since 86.2% of the strains were resistant to at least one antimicrobial and 71.5% displayed multi-resistance profiles. No ampC-producing isolates were detected and only one ESBL-producing E. coli was identified. Genes strA (n=15), floR (n=14), aac(3)IVa (n=13), tetB (n=13), sul2 (n=12), tetA (n=11), aph(3) Ia (n=8) and sul3 (n=5) were detected by PCR. PFGE analysis of these multi-resistant E. coli strains showed less than 80% similarity among them. We conclude that antimicrobial multi-resistant E. coli strains are common on pig carcasses and present highly diverse genotypes and resistance phenotypes and genotypes.
Palavras-Chave: | Resistance genes.
Thesagro: | Escherichia Coli.
Thesaurus Nal: | Antibiotic resistance; Pig carcasses; Pork; Pulsed-field gel electrophoresis.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/172089/1/Genotyping-and-antimicrobial-resistence.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)

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AI-SEDE - (62185-1) - UPE - AP - DD -  -  -  - DIS
CNPSA - (21307-1) - UPC - AP - DD -  -  -  - DIS