Data/hora: 20/06/2024 03:17:18

Biblioteca(s): | Embrapa Meio Ambiente.
Tipo da produção científica: | Nota Técnica/Nota Científica
Data corrente: | 04/01/2021
Data da última atualização: | 04/01/2021
Autoria: | BATISTA, I. C. A.; BOARI, A. de J.; KAUFFMANN, C. M.; NECHET, K. de L.
Afiliação: | IZABEL CRISTINA ALVES BATISTA, UFV; ALESSANDRA DE JESUS BOARI, CPATU; CATERYNNE MELO KAUFFMANN, UFRA; KATIA DE LIMA NECHET, CNPMA.
Título: | Colletotrichum plurivorum causes anthracnose on okra in Brazil.
Ano de publicação: | 2020
Fonte/Imprenta: | Journal of Plant Pathology, v. 102, n. 4, article 1331, 2020
DOI: | https://doi.org/10.1007/s42161-020-00615-1
Idioma: | Inglês
Conteúdo: | Abstract: Okra (Abelmoschus esculentus) is a tropical vegetable cultivated widely in the Amazon region. In August 2016, typical anthracnose symptoms were observed on the leaves of okra in Altamira, Pará, Brazil with 60% disease incidence. Symptomatic leaf tissue were surface sterilized, rinsed with sterile water, and plated on potato dextrose agar. Colonies had greyish white aerial mycelium in 7-day-old cultures at 25±2 °C. Conidiophores were smooth-walled, septate. Conidiogenous cells were hyaline, straight, and 19×4 m. Appressoria were single, elliptical, and 8.5×6.5 m. The conidia were aseptate, cylindrical, with round apex and base, and 13×5 m. The isolated fungus was identified as Colletotrichum sp. The isolate was deposited in Culture Collection of Microorganisms (CMAA), Jaguariúna, Brazil (CMAA 1735). The isolate was further identified by sequencing the actin (ACT), chitin synthase (CHS1), beta-tubulin (TUB) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes (Damm et al. 2019). The sequences were deposited in GenBank (accession Nos. MK495949, MK495952, MK495951 and MK495950) and revealed over 98% identity with Colletotrichum plurivorum (MG747766, MG600847, MG600984 and MK862223). Reference sequences of C. orchidearum and C. magnum species complexes were used for the alignment and the phylogenetic analyses conducted using Bayesian method clustered the isolate in the clade corresponding to Colletotrichum plurivorum. The pathogenicity of the isolate was confirmed by spraying a suspension of conidia (1×106 mL1) on okra cv. Santa Cruz 47 with 46 leaves and the control plants were sprayed with distilled water. The plants were maintained in a dew chamber for 24 h under greenhouse at 27 °C with a 12-h-light/dark photoperiod. After 5 days, dark brown necrotic lesions were observed on the leaves of okra plants whereas control plants were all symptomless. The fungus was re-isolated, and showed the same morphological characteristics as the inoculated strain, fulfilling Kochs postulates. C. plurivorum has been reported to infect papaya (Sun et al. 2019) and cassava (Liu et al. 2019). To our knowledge, this is the first report of Colletotrichum plurivorum causing anthracnose on okra in Brazil.
Palavras-Chave: | Ascomycetes; Fungal pathogen; Vegetable.
Thesagro: | Abelmoschus Esculentus; Antracnose; Doença de Planta; Quiabo.
Thesaurus Nal: | Anthracnose; Fungal diseases of plants; Okra.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16903-1) - UPC - AP - DD -  -  -  - DIS