Data/hora: 21/09/2024 22:29:56

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	14/02/2017	
Data da última atualização: 	30/01/2023	
Autoria: 	BARBOSA, J. O.; ROSSONI, R. D.; VILELA, S. F. G.; ALVARENGA, J. A. de; VELLOSO, M. dos S.; PRATA, M. C. de A.; JORGE, A. O. C.; JUNQUEIRA, J. C.	
Afiliação: 	Júnia Oliveira Barbosa, UNESP; Rodnei Dennis Rossoni, UNESP; Simone Furgeri Godinho Vilela, UNESP; Janaína Araújo de Alvarenga, UNESP; Marisol dos Santos Velloso, UNESP; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Antonio Olavo Cardoso Jorge, UNESP; Juliana Campos Junqueira, UNESP.	
Título: 	Streptococcus mutans can modulate biofilm formation and attenuate the virulence of Candida albicans.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Plos One, v. 11, n. 3, e0150457, 2016.	
Idioma: 	Inglês	
Conteúdo: 	Abstract Streptococcus mutans and Candida albicans are found together in the oral biofilms on dental surfaces, but little is known about the ecological interactions between these species. Here, we studied the effects of S. mutans UA159 on the growth and pathogencity of C. albicans. Initially, the effects of S. mutans on the biofilm formation and morphogenesis of C. albicans were tested in vitro. Next, we investigate the influence of S. mutans on pathogenicity of C. albicans using in vivo host models, in which the experimental candidiasis was induced in G. mellonella larvae and analyzed by survival curves, C. albicans count in hemolymph, and quantification of hyphae in the host tissues. In all the tests, we evaluated the direct effects of S. mutans cells, as well as the indirect effects of the subproducts secreted by this microorganism using a bacterial culture filtrate. The in vitro analysis showed that S. mutans cells favored biofilm formation by C. albicans. However, a reduction in biofilm viable cells and inhibition of hyphal growth was observed when C. albicans was in contact with the S. mutans culture filtrate. In the in vivo study, injection of S. mutans cells or S. mutans culture filtrate into G. mellonella larvae infected with C. albicans increased the survival of these animals. Furthermore, a reduction in hyphal formation was observed in larval tissues when C. albicans was associated with S. mutans culture filtrate. These findings suggest that S. mutans can secrete subproducts capable to inhibit the biofilm formation, morphogenesis and pathogenicity of C. albicans, attenuating the experimental candidiasis in G. mellonella model.	
Palavras-Chave: 	Biofilms; Biosupression; Patology.	
Thesaurus Nal: 	microbiology.	
Categoria do assunto: 	H Saúde e Patologia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/155632/1/Cnpgl-2016-PlosOne-Streptococcus.PDF	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (23286-1) - UPC - AP - DD -  -  -  - DIS